Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01350 and RR42_RS01355 overlap by 1 nucleotides RR42_RS01355 and RR42_RS01360 are separated by 48 nucleotides RR42_RS01360 and RR42_RS01365 are separated by 4 nucleotides RR42_RS01365 and RR42_RS01370 are separated by 93 nucleotides
RR42_RS01350: RR42_RS01350 - biotin carboxylase, at 307,764 to 309,137
_RS01350
RR42_RS01355: RR42_RS01355 - biotin attachment protein, at 309,137 to 309,349
_RS01355
RR42_RS01360: RR42_RS01360 - phenylacetic acid degradation protein, at 309,398 to 309,826
_RS01360
RR42_RS01365: RR42_RS01365 - hypothetical protein, at 309,831 to 310,271
_RS01365
RR42_RS01370: RR42_RS01370 - phosphomethylpyrimidine kinase, at 310,365 to 311,195
_RS01370
Position (kb) 309
310
311 Strain fitness (log2 ratio) -2
-1
0
1
2 at 308.896 kb on - strand, within RR42_RS01350 at 308.896 kb on - strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 309.008 kb on + strand at 309.120 kb on - strand at 309.159 kb on + strand, within RR42_RS01355 at 309.159 kb on + strand, within RR42_RS01355 at 309.269 kb on + strand, within RR42_RS01355 at 309.270 kb on - strand, within RR42_RS01355 at 309.348 kb on - strand at 309.434 kb on + strand at 309.649 kb on + strand, within RR42_RS01360 at 309.649 kb on + strand, within RR42_RS01360 at 309.650 kb on - strand, within RR42_RS01360 at 309.650 kb on - strand, within RR42_RS01360 at 309.892 kb on + strand, within RR42_RS01365 at 310.063 kb on + strand, within RR42_RS01365 at 310.194 kb on + strand, within RR42_RS01365 at 310.195 kb on - strand, within RR42_RS01365 at 310.203 kb on + strand, within RR42_RS01365 at 310.204 kb on - strand, within RR42_RS01365 at 310.219 kb on + strand, within RR42_RS01365 at 310.338 kb on + strand at 310.339 kb on - strand at 310.339 kb on - strand at 310.343 kb on - strand at 310.351 kb on - strand at 310.355 kb on - strand at 310.362 kb on + strand at 310.363 kb on - strand at 310.363 kb on - strand at 310.367 kb on - strand at 311.259 kb on + strand at 311.259 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 308,896 - RR42_RS01350 0.82 -1.4 308,896 - RR42_RS01350 0.82 +0.5 308,898 + RR42_RS01350 0.83 -0.7 308,898 + RR42_RS01350 0.83 +0.3 308,898 + RR42_RS01350 0.83 +0.4 309,008 + +0.2 309,120 - +0.8 309,159 + RR42_RS01355 0.10 +0.4 309,159 + RR42_RS01355 0.10 +0.7 309,269 + RR42_RS01355 0.62 +0.7 309,270 - RR42_RS01355 0.62 +0.4 309,348 - -0.4 309,434 + -0.6 309,649 + RR42_RS01360 0.59 +1.1 309,649 + RR42_RS01360 0.59 -0.2 309,650 - RR42_RS01360 0.59 +0.0 309,650 - RR42_RS01360 0.59 -0.2 309,892 + RR42_RS01365 0.14 +0.8 310,063 + RR42_RS01365 0.53 -0.0 310,194 + RR42_RS01365 0.82 +0.2 310,195 - RR42_RS01365 0.83 -1.2 310,203 + RR42_RS01365 0.84 +0.3 310,204 - RR42_RS01365 0.85 -0.7 310,219 + RR42_RS01365 0.88 -2.6 310,338 + -0.0 310,339 - +1.1 310,339 - +0.7 310,343 - +0.6 310,351 - -0.5 310,355 - +0.3 310,362 + +0.0 310,363 - +1.0 310,363 - +1.8 310,367 - +0.7 311,259 + -0.5 311,259 + +0.4
Or see this region's nucleotide sequence