Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01240 and RR42_RS01245 are separated by 52 nucleotides RR42_RS01245 and RR42_RS01250 are separated by 48 nucleotides
RR42_RS01240: RR42_RS01240 - membrane protein, at 280,383 to 281,735
_RS01240
RR42_RS01245: RR42_RS01245 - polyphosphate kinase, at 281,788 to 282,597
_RS01245
RR42_RS01250: RR42_RS01250 - diaminopimelate epimerase, at 282,646 to 283,512
_RS01250
Position (kb) 281
282
283 Strain fitness (log2 ratio) -1
0
1
2 at 280.788 kb on + strand, within RR42_RS01240 at 280.789 kb on - strand, within RR42_RS01240 at 280.789 kb on - strand, within RR42_RS01240 at 280.827 kb on + strand, within RR42_RS01240 at 280.992 kb on + strand, within RR42_RS01240 at 280.992 kb on + strand, within RR42_RS01240 at 280.995 kb on + strand, within RR42_RS01240 at 280.995 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.119 kb on - strand, within RR42_RS01240 at 281.218 kb on - strand, within RR42_RS01240 at 281.376 kb on + strand, within RR42_RS01240 at 281.377 kb on - strand, within RR42_RS01240 at 281.387 kb on + strand, within RR42_RS01240 at 281.406 kb on + strand, within RR42_RS01240 at 281.407 kb on - strand, within RR42_RS01240 at 281.407 kb on - strand, within RR42_RS01240 at 281.407 kb on - strand, within RR42_RS01240 at 281.407 kb on - strand, within RR42_RS01240 at 281.508 kb on + strand, within RR42_RS01240 at 281.509 kb on - strand, within RR42_RS01240 at 281.509 kb on - strand, within RR42_RS01240 at 281.832 kb on - strand at 281.918 kb on + strand, within RR42_RS01245 at 281.918 kb on + strand, within RR42_RS01245 at 281.918 kb on + strand, within RR42_RS01245 at 281.919 kb on - strand, within RR42_RS01245 at 281.919 kb on - strand, within RR42_RS01245 at 281.961 kb on + strand, within RR42_RS01245 at 281.964 kb on - strand, within RR42_RS01245 at 281.964 kb on - strand, within RR42_RS01245 at 282.145 kb on + strand, within RR42_RS01245 at 282.156 kb on - strand, within RR42_RS01245 at 282.284 kb on + strand, within RR42_RS01245 at 282.440 kb on + strand, within RR42_RS01245 at 282.440 kb on + strand, within RR42_RS01245 at 282.642 kb on - strand at 283.523 kb on + strand at 283.523 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 280,788 + RR42_RS01240 0.30 +1.9 280,789 - RR42_RS01240 0.30 +2.3 280,789 - RR42_RS01240 0.30 +0.9 280,827 + RR42_RS01240 0.33 -0.2 280,992 + RR42_RS01240 0.45 -0.6 280,992 + RR42_RS01240 0.45 +1.3 280,995 + RR42_RS01240 0.45 +0.3 280,995 + RR42_RS01240 0.45 +0.3 281,000 + RR42_RS01240 0.46 -0.3 281,000 + RR42_RS01240 0.46 +0.0 281,000 + RR42_RS01240 0.46 +0.3 281,000 + RR42_RS01240 0.46 +0.2 281,000 + RR42_RS01240 0.46 +0.1 281,119 - RR42_RS01240 0.54 -0.1 281,218 - RR42_RS01240 0.62 +0.4 281,376 + RR42_RS01240 0.73 +0.2 281,377 - RR42_RS01240 0.73 -0.4 281,387 + RR42_RS01240 0.74 -0.6 281,406 + RR42_RS01240 0.76 -0.1 281,407 - RR42_RS01240 0.76 -0.7 281,407 - RR42_RS01240 0.76 +0.3 281,407 - RR42_RS01240 0.76 -1.0 281,407 - RR42_RS01240 0.76 -0.1 281,508 + RR42_RS01240 0.83 +0.7 281,509 - RR42_RS01240 0.83 +1.1 281,509 - RR42_RS01240 0.83 +0.0 281,832 - +0.6 281,918 + RR42_RS01245 0.16 -0.8 281,918 + RR42_RS01245 0.16 +0.3 281,918 + RR42_RS01245 0.16 -0.2 281,919 - RR42_RS01245 0.16 +0.2 281,919 - RR42_RS01245 0.16 -1.1 281,961 + RR42_RS01245 0.21 +0.4 281,964 - RR42_RS01245 0.22 +0.2 281,964 - RR42_RS01245 0.22 +0.3 282,145 + RR42_RS01245 0.44 +0.1 282,156 - RR42_RS01245 0.45 +0.2 282,284 + RR42_RS01245 0.61 +1.0 282,440 + RR42_RS01245 0.80 +1.3 282,440 + RR42_RS01245 0.80 -0.6 282,642 - +0.5 283,523 + -0.1 283,523 + +0.3
Or see this region's nucleotide sequence