Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01075 and RR42_RS01080 are separated by 259 nucleotides RR42_RS01080 and RR42_RS01085 are separated by 23 nucleotides RR42_RS01085 and RR42_RS01090 overlap by 4 nucleotides
RR42_RS01075: RR42_RS01075 - hypothetical protein, at 252,492 to 253,073
_RS01075
RR42_RS01080: RR42_RS01080 - hypothetical protein, at 253,333 to 253,629
_RS01080
RR42_RS01085: RR42_RS01085 - chemotaxis protein CheY, at 253,653 to 254,276
_RS01085
RR42_RS01090: RR42_RS01090 - histidine kinase, at 254,273 to 255,676
_RS01090
Position (kb) 253
254 Strain fitness (log2 ratio) -2
-1
0
1
2 at 252.393 kb on - strand at 252.393 kb on - strand at 252.399 kb on + strand at 252.399 kb on + strand at 252.400 kb on - strand at 252.403 kb on + strand at 252.403 kb on + strand at 252.403 kb on + strand at 252.403 kb on + strand at 252.403 kb on + strand at 252.403 kb on + strand at 252.403 kb on + strand at 252.403 kb on + strand at 252.403 kb on + strand at 252.404 kb on - strand at 252.404 kb on - strand at 252.404 kb on - strand at 252.404 kb on - strand at 252.404 kb on - strand at 252.404 kb on - strand at 252.404 kb on - strand at 252.404 kb on - strand at 252.594 kb on + strand, within RR42_RS01075 at 252.595 kb on - strand, within RR42_RS01075 at 252.627 kb on + strand, within RR42_RS01075 at 252.627 kb on + strand, within RR42_RS01075 at 252.628 kb on - strand, within RR42_RS01075 at 252.628 kb on - strand, within RR42_RS01075 at 252.628 kb on - strand, within RR42_RS01075 at 252.628 kb on - strand, within RR42_RS01075 at 252.795 kb on + strand, within RR42_RS01075 at 252.796 kb on - strand, within RR42_RS01075 at 252.853 kb on + strand, within RR42_RS01075 at 252.853 kb on + strand, within RR42_RS01075 at 252.854 kb on - strand, within RR42_RS01075 at 252.985 kb on - strand, within RR42_RS01075 at 252.985 kb on - strand, within RR42_RS01075 at 252.985 kb on - strand, within RR42_RS01075 at 253.034 kb on + strand at 253.034 kb on + strand at 253.034 kb on + strand at 253.035 kb on - strand at 253.035 kb on - strand at 253.062 kb on + strand at 253.071 kb on + strand at 253.071 kb on + strand at 253.071 kb on + strand at 253.071 kb on + strand at 253.071 kb on + strand at 253.072 kb on - strand at 253.072 kb on - strand at 253.158 kb on + strand at 253.159 kb on - strand at 253.172 kb on - strand at 253.205 kb on + strand at 253.205 kb on + strand at 253.206 kb on - strand at 253.206 kb on - strand at 253.206 kb on - strand at 253.216 kb on - strand at 253.346 kb on + strand at 253.346 kb on + strand at 253.346 kb on + strand at 253.346 kb on + strand at 253.347 kb on - strand at 253.347 kb on - strand at 253.347 kb on - strand at 253.425 kb on - strand, within RR42_RS01080 at 253.609 kb on + strand at 253.609 kb on + strand at 253.610 kb on - strand at 253.610 kb on - strand at 253.610 kb on - strand at 253.610 kb on - strand at 253.610 kb on - strand at 253.637 kb on + strand at 253.638 kb on - strand at 253.654 kb on + strand at 253.655 kb on - strand at 253.706 kb on + strand at 253.706 kb on + strand at 253.706 kb on + strand at 253.706 kb on + strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.733 kb on + strand, within RR42_RS01085 at 253.733 kb on + strand, within RR42_RS01085 at 253.733 kb on + strand, within RR42_RS01085 at 253.733 kb on + strand, within RR42_RS01085 at 253.734 kb on - strand, within RR42_RS01085 at 253.894 kb on + strand, within RR42_RS01085 at 253.895 kb on - strand, within RR42_RS01085 at 253.895 kb on - strand, within RR42_RS01085 at 253.945 kb on + strand, within RR42_RS01085 at 253.945 kb on + strand, within RR42_RS01085 at 254.077 kb on + strand, within RR42_RS01085 at 254.077 kb on + strand, within RR42_RS01085 at 254.078 kb on - strand, within RR42_RS01085 at 254.433 kb on + strand, within RR42_RS01090 at 254.434 kb on - strand, within RR42_RS01090
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 252,393 - +2.3 252,393 - -1.0 252,399 + +0.2 252,399 + +1.4 252,400 - -0.5 252,403 + -0.5 252,403 + -0.1 252,403 + +1.0 252,403 + +0.3 252,403 + +1.0 252,403 + -0.3 252,403 + +0.3 252,403 + +0.2 252,403 + -1.0 252,404 - +0.3 252,404 - -0.9 252,404 - -0.7 252,404 - -0.2 252,404 - -1.6 252,404 - -0.9 252,404 - +0.6 252,404 - -0.6 252,594 + RR42_RS01075 0.18 +0.2 252,595 - RR42_RS01075 0.18 +2.1 252,627 + RR42_RS01075 0.23 -0.3 252,627 + RR42_RS01075 0.23 +0.3 252,628 - RR42_RS01075 0.23 +1.1 252,628 - RR42_RS01075 0.23 -0.2 252,628 - RR42_RS01075 0.23 +1.0 252,628 - RR42_RS01075 0.23 +2.5 252,795 + RR42_RS01075 0.52 -1.3 252,796 - RR42_RS01075 0.52 -0.6 252,853 + RR42_RS01075 0.62 +1.3 252,853 + RR42_RS01075 0.62 -0.1 252,854 - RR42_RS01075 0.62 +1.9 252,985 - RR42_RS01075 0.85 -0.1 252,985 - RR42_RS01075 0.85 +1.3 252,985 - RR42_RS01075 0.85 -0.3 253,034 + +0.2 253,034 + +1.2 253,034 + -0.7 253,035 - +1.2 253,035 - +1.8 253,062 + +0.0 253,071 + -0.2 253,071 + +1.1 253,071 + +2.0 253,071 + +0.3 253,071 + -0.2 253,072 - -0.2 253,072 - -0.4 253,158 + +1.4 253,159 - +0.5 253,172 - -0.6 253,205 + +0.8 253,205 + +0.9 253,206 - +0.7 253,206 - +0.1 253,206 - -0.3 253,216 - -0.9 253,346 + -0.7 253,346 + +0.7 253,346 + +0.4 253,346 + +0.2 253,347 - -0.0 253,347 - -0.4 253,347 - -0.1 253,425 - RR42_RS01080 0.31 +0.7 253,609 + -1.4 253,609 + +1.6 253,610 - -0.0 253,610 - -0.0 253,610 - -0.2 253,610 - +0.1 253,610 - -0.7 253,637 + +0.8 253,638 - +0.5 253,654 + -0.9 253,655 - +0.8 253,706 + -1.5 253,706 + -0.5 253,706 + +0.9 253,706 + -1.5 253,707 - -1.5 253,707 - -2.0 253,707 - +0.3 253,707 - -2.5 253,707 - -1.5 253,707 - -0.7 253,733 + RR42_RS01085 0.13 -1.0 253,733 + RR42_RS01085 0.13 -1.2 253,733 + RR42_RS01085 0.13 -0.7 253,733 + RR42_RS01085 0.13 -1.3 253,734 - RR42_RS01085 0.13 +0.7 253,894 + RR42_RS01085 0.39 -0.6 253,895 - RR42_RS01085 0.39 +1.8 253,895 - RR42_RS01085 0.39 +0.6 253,945 + RR42_RS01085 0.47 -2.2 253,945 + RR42_RS01085 0.47 -2.3 254,077 + RR42_RS01085 0.68 -1.1 254,077 + RR42_RS01085 0.68 -1.1 254,078 - RR42_RS01085 0.68 -1.6 254,433 + RR42_RS01090 0.11 +0.3 254,434 - RR42_RS01090 0.11 -2.6
Or see this region's nucleotide sequence