Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00755 and RR42_RS00760 are separated by 251 nucleotides RR42_RS00760 and RR42_RS00765 are separated by 149 nucleotides RR42_RS00765 and RR42_RS00770 are separated by 177 nucleotides
RR42_RS00755: RR42_RS00755 - 3-oxoadipate enol-lactonase, at 189,546 to 190,388
_RS00755
RR42_RS00760: RR42_RS00760 - hypothetical protein, at 190,640 to 190,897
_RS00760
RR42_RS00765: RR42_RS00765 - sulfurtransferase, at 191,047 to 191,439
_RS00765
RR42_RS00770: RR42_RS00770 - 5-methyltetrahydrofolate--homocysteine methyltransferase, at 191,617 to 192,684
_RS00770
Position (kb) 190
191 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 189.727 kb on - strand, within RR42_RS00755 at 189.772 kb on - strand, within RR42_RS00755 at 189.772 kb on - strand, within RR42_RS00755 at 190.184 kb on + strand, within RR42_RS00755 at 190.184 kb on + strand, within RR42_RS00755 at 190.305 kb on - strand at 190.381 kb on + strand at 190.381 kb on + strand at 190.381 kb on + strand at 190.381 kb on + strand at 190.382 kb on - strand at 190.382 kb on - strand at 190.382 kb on - strand at 190.475 kb on + strand at 190.475 kb on + strand at 190.475 kb on + strand at 190.475 kb on + strand at 190.475 kb on + strand at 190.476 kb on - strand at 190.490 kb on + strand at 190.634 kb on + strand at 190.635 kb on - strand at 190.635 kb on - strand at 190.653 kb on - strand at 190.653 kb on - strand at 190.725 kb on - strand, within RR42_RS00760 at 190.864 kb on + strand, within RR42_RS00760 at 190.864 kb on + strand, within RR42_RS00760 at 190.865 kb on - strand, within RR42_RS00760 at 190.865 kb on - strand, within RR42_RS00760 at 190.895 kb on + strand at 190.896 kb on - strand at 190.896 kb on - strand at 190.964 kb on - strand at 190.998 kb on + strand at 190.998 kb on + strand at 191.051 kb on + strand at 191.051 kb on + strand at 191.052 kb on - strand at 191.052 kb on - strand at 191.052 kb on - strand at 191.094 kb on + strand, within RR42_RS00765 at 191.094 kb on + strand, within RR42_RS00765 at 191.094 kb on + strand, within RR42_RS00765 at 191.094 kb on + strand, within RR42_RS00765 at 191.095 kb on - strand, within RR42_RS00765 at 191.095 kb on - strand, within RR42_RS00765 at 191.147 kb on + strand, within RR42_RS00765 at 191.148 kb on - strand, within RR42_RS00765 at 191.351 kb on + strand, within RR42_RS00765 at 191.351 kb on + strand, within RR42_RS00765 at 191.352 kb on - strand, within RR42_RS00765 at 191.352 kb on - strand, within RR42_RS00765 at 191.352 kb on - strand, within RR42_RS00765 at 191.374 kb on + strand, within RR42_RS00765 at 191.487 kb on + strand at 191.573 kb on + strand at 191.573 kb on + strand at 191.573 kb on + strand at 191.574 kb on - strand at 191.574 kb on - strand at 191.662 kb on + strand at 191.663 kb on - strand at 191.746 kb on + strand, within RR42_RS00770 at 191.746 kb on + strand, within RR42_RS00770 at 191.747 kb on - strand, within RR42_RS00770 at 191.747 kb on - strand, within RR42_RS00770 at 191.747 kb on - strand, within RR42_RS00770 at 191.832 kb on - strand, within RR42_RS00770 at 191.869 kb on + strand, within RR42_RS00770 at 191.870 kb on - strand, within RR42_RS00770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 189,727 - RR42_RS00755 0.21 +0.0 189,772 - RR42_RS00755 0.27 -0.0 189,772 - RR42_RS00755 0.27 -0.3 190,184 + RR42_RS00755 0.76 +0.2 190,184 + RR42_RS00755 0.76 -0.7 190,305 - +0.2 190,381 + +0.6 190,381 + +2.3 190,381 + -0.2 190,381 + +0.6 190,382 - -1.2 190,382 - +0.7 190,382 - +0.4 190,475 + -0.7 190,475 + -0.9 190,475 + +0.2 190,475 + +2.3 190,475 + +1.4 190,476 - +0.0 190,490 + -1.3 190,634 + +0.5 190,635 - -0.4 190,635 - +1.1 190,653 - -0.1 190,653 - -0.0 190,725 - RR42_RS00760 0.33 -0.7 190,864 + RR42_RS00760 0.87 -2.4 190,864 + RR42_RS00760 0.87 -0.5 190,865 - RR42_RS00760 0.87 -1.0 190,865 - RR42_RS00760 0.87 +2.9 190,895 + +0.6 190,896 - +0.5 190,896 - +0.2 190,964 - +1.3 190,998 + +0.5 190,998 + +0.1 191,051 + +0.3 191,051 + +0.8 191,052 - -0.3 191,052 - +0.1 191,052 - -1.7 191,094 + RR42_RS00765 0.12 +0.1 191,094 + RR42_RS00765 0.12 -1.5 191,094 + RR42_RS00765 0.12 -0.4 191,094 + RR42_RS00765 0.12 +0.3 191,095 - RR42_RS00765 0.12 -0.3 191,095 - RR42_RS00765 0.12 +0.3 191,147 + RR42_RS00765 0.25 -0.1 191,148 - RR42_RS00765 0.26 +1.4 191,351 + RR42_RS00765 0.77 -1.7 191,351 + RR42_RS00765 0.77 -0.3 191,352 - RR42_RS00765 0.78 -0.4 191,352 - RR42_RS00765 0.78 +0.6 191,352 - RR42_RS00765 0.78 -0.8 191,374 + RR42_RS00765 0.83 -0.9 191,487 + +0.5 191,573 + -0.0 191,573 + +1.7 191,573 + -0.5 191,574 - -0.4 191,574 - +0.6 191,662 + +0.0 191,663 - -0.7 191,746 + RR42_RS00770 0.12 +0.1 191,746 + RR42_RS00770 0.12 -0.0 191,747 - RR42_RS00770 0.12 +0.1 191,747 - RR42_RS00770 0.12 +0.0 191,747 - RR42_RS00770 0.12 +1.1 191,832 - RR42_RS00770 0.20 -0.6 191,869 + RR42_RS00770 0.24 -0.9 191,870 - RR42_RS00770 0.24 +1.3
Or see this region's nucleotide sequence