Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00740 and RR42_RS00745 are separated by 340 nucleotides RR42_RS00745 and RR42_RS00750 are separated by 58 nucleotides
RR42_RS00740: RR42_RS00740 - ABC transporter substrate-binding protein, at 186,250 to 187,206
_RS00740
RR42_RS00745: RR42_RS00745 - IclR family transcriptional regulator, at 187,547 to 188,383
_RS00745
RR42_RS00750: RR42_RS00750 - hypothetical protein, at 188,442 to 189,422
_RS00750
Position (kb) 187
188
189 Strain fitness (log2 ratio) -2
-1
0
1 at 186.672 kb on - strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.897 kb on - strand, within RR42_RS00740 at 186.897 kb on - strand, within RR42_RS00740 at 186.911 kb on + strand, within RR42_RS00740 at 186.962 kb on + strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 187.202 kb on - strand at 187.286 kb on - strand at 187.358 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.444 kb on + strand at 187.445 kb on - strand at 187.445 kb on - strand at 187.446 kb on + strand at 187.450 kb on - strand at 187.450 kb on - strand at 187.498 kb on - strand at 187.498 kb on - strand at 187.507 kb on + strand at 187.507 kb on + strand at 187.508 kb on - strand at 187.508 kb on - strand at 187.706 kb on + strand, within RR42_RS00745 at 187.764 kb on - strand, within RR42_RS00745 at 188.015 kb on + strand, within RR42_RS00745 at 188.016 kb on - strand, within RR42_RS00745 at 188.016 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.111 kb on + strand at 188.111 kb on + strand, within RR42_RS00745 at 188.112 kb on - strand, within RR42_RS00745 at 188.112 kb on - strand, within RR42_RS00745 at 188.259 kb on + strand, within RR42_RS00745 at 188.259 kb on + strand, within RR42_RS00745 at 188.260 kb on - strand, within RR42_RS00745 at 188.336 kb on + strand at 188.336 kb on + strand at 188.468 kb on + strand at 188.468 kb on + strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.883 kb on + strand, within RR42_RS00750 at 189.145 kb on - strand, within RR42_RS00750 at 189.145 kb on - strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.239 kb on + strand, within RR42_RS00750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 186,672 - RR42_RS00740 0.44 +1.1 186,788 + RR42_RS00740 0.56 +0.8 186,788 + RR42_RS00740 0.56 -1.0 186,788 + RR42_RS00740 0.56 +0.2 186,788 + RR42_RS00740 0.56 +0.8 186,789 - RR42_RS00740 0.56 -0.1 186,789 - RR42_RS00740 0.56 +0.2 186,789 - RR42_RS00740 0.56 +0.1 186,897 - RR42_RS00740 0.68 -0.0 186,897 - RR42_RS00740 0.68 -0.3 186,911 + RR42_RS00740 0.69 +1.1 186,962 + RR42_RS00740 0.74 +0.9 186,963 - RR42_RS00740 0.75 -0.3 186,963 - RR42_RS00740 0.75 -1.4 186,963 - RR42_RS00740 0.75 +0.7 187,202 - -0.1 187,286 - -0.3 187,358 + +0.2 187,378 + +0.3 187,378 + -1.1 187,378 + -0.3 187,378 + -0.4 187,378 + -1.6 187,378 + -0.1 187,378 + +1.3 187,378 + +1.3 187,379 - +1.3 187,379 - -0.0 187,379 - -0.7 187,379 - +0.8 187,380 + -0.1 187,380 + +0.0 187,380 + +0.4 187,380 + +0.6 187,380 + -0.3 187,380 + -0.4 187,380 + +0.1 187,380 + -0.4 187,380 + -0.2 187,380 + -0.3 187,380 + +0.4 187,380 + -0.7 187,381 - -1.0 187,381 - -1.5 187,381 - +0.6 187,381 - -0.0 187,381 - -0.0 187,381 - -0.2 187,381 - +0.3 187,381 - -0.6 187,381 - +1.3 187,444 + -0.3 187,445 - -1.0 187,445 - -0.5 187,446 + +0.2 187,450 - -0.0 187,450 - +0.3 187,498 - +0.6 187,498 - -0.4 187,507 + +1.3 187,507 + -0.3 187,508 - +0.4 187,508 - -0.5 187,706 + RR42_RS00745 0.19 -1.0 187,764 - RR42_RS00745 0.26 -0.3 188,015 + RR42_RS00745 0.56 +0.7 188,016 - RR42_RS00745 0.56 -0.7 188,016 - RR42_RS00745 0.56 +0.7 188,081 - RR42_RS00745 0.64 +0.7 188,081 - RR42_RS00745 0.64 -0.7 188,081 - RR42_RS00745 0.64 -0.2 188,081 - RR42_RS00745 0.64 -0.7 188,111 + -0.3 188,111 + RR42_RS00745 0.67 -1.2 188,112 - RR42_RS00745 0.68 -1.2 188,112 - RR42_RS00745 0.68 -0.1 188,259 + RR42_RS00745 0.85 -0.0 188,259 + RR42_RS00745 0.85 -1.0 188,260 - RR42_RS00745 0.85 -0.8 188,336 + +0.1 188,336 + +0.0 188,468 + +0.1 188,468 + +0.2 188,469 - +0.9 188,469 - +0.5 188,883 + RR42_RS00750 0.45 -0.4 189,145 - RR42_RS00750 0.72 -1.0 189,145 - RR42_RS00750 0.72 +1.7 189,216 + RR42_RS00750 0.79 +0.7 189,216 + RR42_RS00750 0.79 +0.5 189,216 + RR42_RS00750 0.79 -1.3 189,216 + RR42_RS00750 0.79 -0.6 189,216 + RR42_RS00750 0.79 -0.8 189,216 + RR42_RS00750 0.79 -0.7 189,216 + RR42_RS00750 0.79 +0.3 189,216 + RR42_RS00750 0.79 -0.7 189,216 + RR42_RS00750 0.79 -2.7 189,217 - RR42_RS00750 0.79 +0.7 189,217 - RR42_RS00750 0.79 -0.5 189,217 - RR42_RS00750 0.79 -0.6 189,239 + RR42_RS00750 0.81 -1.5
Or see this region's nucleotide sequence