Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00730 and RR42_RS00735 are separated by 265 nucleotides RR42_RS00735 and RR42_RS00740 are separated by 66 nucleotides
RR42_RS00730: RR42_RS00730 - enoyl-CoA hydratase, at 184,116 to 184,934
_RS00730
RR42_RS00735: RR42_RS00735 - fumarylacetoacetate hydrolase, at 185,200 to 186,183
_RS00735
RR42_RS00740: RR42_RS00740 - ABC transporter substrate-binding protein, at 186,250 to 187,206
_RS00740
Position (kb)
185
186
187 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 184.244 kb on + strand, within RR42_RS00730 at 184.245 kb on - strand, within RR42_RS00730 at 184.255 kb on + strand, within RR42_RS00730 at 184.255 kb on + strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.487 kb on + strand, within RR42_RS00730 at 184.487 kb on + strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.618 kb on + strand, within RR42_RS00730 at 184.618 kb on + strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.799 kb on + strand, within RR42_RS00730 at 184.799 kb on + strand, within RR42_RS00730 at 184.800 kb on - strand, within RR42_RS00730 at 185.360 kb on + strand, within RR42_RS00735 at 185.440 kb on - strand, within RR42_RS00735 at 185.440 kb on - strand, within RR42_RS00735 at 185.585 kb on - strand, within RR42_RS00735 at 185.585 kb on - strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.803 kb on - strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 186.026 kb on + strand, within RR42_RS00735 at 186.027 kb on - strand, within RR42_RS00735 at 186.027 kb on - strand, within RR42_RS00735 at 186.027 kb on - strand, within RR42_RS00735 at 186.104 kb on + strand at 186.104 kb on + strand at 186.105 kb on - strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.186 kb on - strand at 186.186 kb on - strand at 186.186 kb on - strand at 186.186 kb on - strand at 186.186 kb on - strand at 186.197 kb on + strand at 186.252 kb on - strand at 186.476 kb on + strand, within RR42_RS00740 at 186.477 kb on - strand, within RR42_RS00740 at 186.477 kb on - strand, within RR42_RS00740 at 186.477 kb on - strand, within RR42_RS00740 at 186.672 kb on - strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.897 kb on - strand, within RR42_RS00740 at 186.897 kb on - strand, within RR42_RS00740 at 186.911 kb on + strand, within RR42_RS00740 at 186.962 kb on + strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 184,244 + RR42_RS00730 0.16 +0.2 184,245 - RR42_RS00730 0.16 -1.3 184,255 + RR42_RS00730 0.17 -0.3 184,255 + RR42_RS00730 0.17 -0.3 184,256 - RR42_RS00730 0.17 +0.1 184,256 - RR42_RS00730 0.17 -1.7 184,256 - RR42_RS00730 0.17 -0.6 184,408 + RR42_RS00730 0.36 -0.3 184,408 + RR42_RS00730 0.36 -0.3 184,408 + RR42_RS00730 0.36 -0.1 184,409 - RR42_RS00730 0.36 +0.7 184,409 - RR42_RS00730 0.36 +0.1 184,409 - RR42_RS00730 0.36 +0.2 184,409 - RR42_RS00730 0.36 -0.2 184,411 + RR42_RS00730 0.36 -0.5 184,411 + RR42_RS00730 0.36 -0.6 184,411 + RR42_RS00730 0.36 -0.0 184,411 + RR42_RS00730 0.36 -0.0 184,411 + RR42_RS00730 0.36 +0.9 184,411 + RR42_RS00730 0.36 +0.2 184,411 + RR42_RS00730 0.36 +0.9 184,411 + RR42_RS00730 0.36 -0.4 184,412 - RR42_RS00730 0.36 -0.7 184,412 - RR42_RS00730 0.36 -0.4 184,412 - RR42_RS00730 0.36 -1.2 184,487 + RR42_RS00730 0.45 +0.3 184,487 + RR42_RS00730 0.45 -0.9 184,530 - RR42_RS00730 0.51 +0.5 184,530 - RR42_RS00730 0.51 -0.2 184,530 - RR42_RS00730 0.51 -2.0 184,618 + RR42_RS00730 0.61 -1.2 184,618 + RR42_RS00730 0.61 -1.3 184,619 - RR42_RS00730 0.61 +0.6 184,619 - RR42_RS00730 0.61 +0.1 184,619 - RR42_RS00730 0.61 -1.3 184,799 + RR42_RS00730 0.83 -0.5 184,799 + RR42_RS00730 0.83 +0.9 184,800 - RR42_RS00730 0.84 -0.0 185,360 + RR42_RS00735 0.16 -0.4 185,440 - RR42_RS00735 0.24 +0.5 185,440 - RR42_RS00735 0.24 +0.4 185,585 - RR42_RS00735 0.39 -0.6 185,585 - RR42_RS00735 0.39 -0.0 185,668 + RR42_RS00735 0.48 -0.0 185,668 + RR42_RS00735 0.48 -0.0 185,668 + RR42_RS00735 0.48 -1.2 185,668 + RR42_RS00735 0.48 -0.2 185,668 + RR42_RS00735 0.48 -0.3 185,669 - RR42_RS00735 0.48 -0.2 185,669 - RR42_RS00735 0.48 +0.1 185,669 - RR42_RS00735 0.48 +0.3 185,669 - RR42_RS00735 0.48 +0.7 185,803 - RR42_RS00735 0.61 +0.4 185,843 + RR42_RS00735 0.65 +0.8 185,843 + RR42_RS00735 0.65 +0.5 185,843 + RR42_RS00735 0.65 +0.6 185,844 - RR42_RS00735 0.65 -0.9 185,844 - RR42_RS00735 0.65 +0.8 185,844 - RR42_RS00735 0.65 -0.7 185,844 - RR42_RS00735 0.65 -0.3 186,026 + RR42_RS00735 0.84 +0.1 186,027 - RR42_RS00735 0.84 -0.3 186,027 - RR42_RS00735 0.84 +0.6 186,027 - RR42_RS00735 0.84 -0.7 186,104 + +0.2 186,104 + +0.3 186,105 - -0.2 186,185 + +0.6 186,185 + -1.9 186,185 + +1.8 186,185 + -1.3 186,185 + +0.4 186,185 + -0.2 186,186 - -0.1 186,186 - +0.1 186,186 - +0.1 186,186 - +0.6 186,186 - +0.7 186,197 + -0.1 186,252 - +0.2 186,476 + RR42_RS00740 0.24 +1.1 186,477 - RR42_RS00740 0.24 -1.3 186,477 - RR42_RS00740 0.24 +0.9 186,477 - RR42_RS00740 0.24 +0.9 186,672 - RR42_RS00740 0.44 +1.1 186,788 + RR42_RS00740 0.56 +0.8 186,788 + RR42_RS00740 0.56 -1.0 186,788 + RR42_RS00740 0.56 +0.2 186,788 + RR42_RS00740 0.56 +0.8 186,789 - RR42_RS00740 0.56 -0.1 186,789 - RR42_RS00740 0.56 +0.2 186,789 - RR42_RS00740 0.56 +0.1 186,897 - RR42_RS00740 0.68 -0.0 186,897 - RR42_RS00740 0.68 -0.3 186,911 + RR42_RS00740 0.69 +1.1 186,962 + RR42_RS00740 0.74 +0.9 186,963 - RR42_RS00740 0.75 -0.3 186,963 - RR42_RS00740 0.75 -1.4 186,963 - RR42_RS00740 0.75 +0.7
Or see this region's nucleotide sequence