Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00725 and RR42_RS00730 are separated by 69 nucleotides RR42_RS00730 and RR42_RS00735 are separated by 265 nucleotides
RR42_RS00725: RR42_RS00725 - hypothetical protein, at 183,138 to 184,046
_RS00725
RR42_RS00730: RR42_RS00730 - enoyl-CoA hydratase, at 184,116 to 184,934
_RS00730
RR42_RS00735: RR42_RS00735 - fumarylacetoacetate hydrolase, at 185,200 to 186,183
_RS00735
Position (kb)
184
185 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 183.159 kb on - strand at 183.450 kb on - strand, within RR42_RS00725 at 183.657 kb on - strand, within RR42_RS00725 at 183.657 kb on - strand, within RR42_RS00725 at 183.706 kb on + strand, within RR42_RS00725 at 183.706 kb on + strand, within RR42_RS00725 at 183.707 kb on - strand, within RR42_RS00725 at 184.108 kb on + strand at 184.108 kb on + strand at 184.109 kb on - strand at 184.109 kb on - strand at 184.109 kb on - strand at 184.109 kb on - strand at 184.155 kb on + strand at 184.156 kb on - strand at 184.244 kb on + strand, within RR42_RS00730 at 184.245 kb on - strand, within RR42_RS00730 at 184.255 kb on + strand, within RR42_RS00730 at 184.255 kb on + strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.256 kb on - strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.408 kb on + strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.409 kb on - strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.411 kb on + strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.412 kb on - strand, within RR42_RS00730 at 184.487 kb on + strand, within RR42_RS00730 at 184.487 kb on + strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.530 kb on - strand, within RR42_RS00730 at 184.618 kb on + strand, within RR42_RS00730 at 184.618 kb on + strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.619 kb on - strand, within RR42_RS00730 at 184.799 kb on + strand, within RR42_RS00730 at 184.799 kb on + strand, within RR42_RS00730 at 184.800 kb on - strand, within RR42_RS00730 at 185.360 kb on + strand, within RR42_RS00735 at 185.440 kb on - strand, within RR42_RS00735 at 185.440 kb on - strand, within RR42_RS00735 at 185.585 kb on - strand, within RR42_RS00735 at 185.585 kb on - strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.803 kb on - strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 183,159 - -1.4 183,450 - RR42_RS00725 0.34 -0.2 183,657 - RR42_RS00725 0.57 -0.4 183,657 - RR42_RS00725 0.57 +0.4 183,706 + RR42_RS00725 0.62 +0.2 183,706 + RR42_RS00725 0.62 -0.4 183,707 - RR42_RS00725 0.63 -2.3 184,108 + +1.3 184,108 + -0.3 184,109 - +0.5 184,109 - -0.9 184,109 - -3.1 184,109 - +0.8 184,155 + -1.9 184,156 - -0.1 184,244 + RR42_RS00730 0.16 +0.2 184,245 - RR42_RS00730 0.16 -1.3 184,255 + RR42_RS00730 0.17 -0.3 184,255 + RR42_RS00730 0.17 -0.3 184,256 - RR42_RS00730 0.17 +0.1 184,256 - RR42_RS00730 0.17 -1.7 184,256 - RR42_RS00730 0.17 -0.6 184,408 + RR42_RS00730 0.36 -0.3 184,408 + RR42_RS00730 0.36 -0.3 184,408 + RR42_RS00730 0.36 -0.1 184,409 - RR42_RS00730 0.36 +0.7 184,409 - RR42_RS00730 0.36 +0.1 184,409 - RR42_RS00730 0.36 +0.2 184,409 - RR42_RS00730 0.36 -0.2 184,411 + RR42_RS00730 0.36 -0.5 184,411 + RR42_RS00730 0.36 -0.6 184,411 + RR42_RS00730 0.36 -0.0 184,411 + RR42_RS00730 0.36 -0.0 184,411 + RR42_RS00730 0.36 +0.9 184,411 + RR42_RS00730 0.36 +0.2 184,411 + RR42_RS00730 0.36 +0.9 184,411 + RR42_RS00730 0.36 -0.4 184,412 - RR42_RS00730 0.36 -0.7 184,412 - RR42_RS00730 0.36 -0.4 184,412 - RR42_RS00730 0.36 -1.2 184,487 + RR42_RS00730 0.45 +0.3 184,487 + RR42_RS00730 0.45 -0.9 184,530 - RR42_RS00730 0.51 +0.5 184,530 - RR42_RS00730 0.51 -0.2 184,530 - RR42_RS00730 0.51 -2.0 184,618 + RR42_RS00730 0.61 -1.2 184,618 + RR42_RS00730 0.61 -1.3 184,619 - RR42_RS00730 0.61 +0.6 184,619 - RR42_RS00730 0.61 +0.1 184,619 - RR42_RS00730 0.61 -1.3 184,799 + RR42_RS00730 0.83 -0.5 184,799 + RR42_RS00730 0.83 +0.9 184,800 - RR42_RS00730 0.84 -0.0 185,360 + RR42_RS00735 0.16 -0.4 185,440 - RR42_RS00735 0.24 +0.5 185,440 - RR42_RS00735 0.24 +0.4 185,585 - RR42_RS00735 0.39 -0.6 185,585 - RR42_RS00735 0.39 -0.0 185,668 + RR42_RS00735 0.48 -0.0 185,668 + RR42_RS00735 0.48 -0.0 185,668 + RR42_RS00735 0.48 -1.2 185,668 + RR42_RS00735 0.48 -0.2 185,668 + RR42_RS00735 0.48 -0.3 185,669 - RR42_RS00735 0.48 -0.2 185,669 - RR42_RS00735 0.48 +0.1 185,669 - RR42_RS00735 0.48 +0.3 185,669 - RR42_RS00735 0.48 +0.7 185,803 - RR42_RS00735 0.61 +0.4 185,843 + RR42_RS00735 0.65 +0.8 185,843 + RR42_RS00735 0.65 +0.5 185,843 + RR42_RS00735 0.65 +0.6 185,844 - RR42_RS00735 0.65 -0.9 185,844 - RR42_RS00735 0.65 +0.8 185,844 - RR42_RS00735 0.65 -0.7 185,844 - RR42_RS00735 0.65 -0.3
Or see this region's nucleotide sequence