Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00290 and RR42_RS00295 are separated by 103 nucleotides RR42_RS00295 and RR42_RS00300 are separated by 64 nucleotides
RR42_RS00290: RR42_RS00290 - sialidase, at 83,329 to 84,483
_RS00290
RR42_RS00295: RR42_RS00295 - DEAD/DEAH box helicase, at 84,587 to 86,008
_RS00295
RR42_RS00300: RR42_RS00300 - kinase, at 86,073 to 86,921
_RS00300
Position (kb) 84
85
86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 83.986 kb on + strand, within RR42_RS00290 at 83.986 kb on + strand, within RR42_RS00290 at 83.987 kb on - strand, within RR42_RS00290 at 83.987 kb on - strand, within RR42_RS00290 at 83.987 kb on - strand, within RR42_RS00290 at 84.322 kb on + strand, within RR42_RS00290 at 84.334 kb on + strand, within RR42_RS00290 at 84.334 kb on + strand, within RR42_RS00290 at 84.335 kb on - strand, within RR42_RS00290 at 84.463 kb on + strand at 84.463 kb on + strand at 84.464 kb on - strand at 84.505 kb on + strand at 84.506 kb on - strand at 84.576 kb on + strand at 84.997 kb on + strand, within RR42_RS00295 at 84.997 kb on + strand, within RR42_RS00295 at 84.998 kb on - strand, within RR42_RS00295 at 85.130 kb on + strand, within RR42_RS00295 at 85.130 kb on + strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.651 kb on + strand, within RR42_RS00295 at 85.691 kb on - strand, within RR42_RS00295 at 85.859 kb on + strand, within RR42_RS00295 at 85.907 kb on + strand at 85.907 kb on + strand at 85.907 kb on + strand at 85.908 kb on - strand at 86.166 kb on + strand, within RR42_RS00300 at 86.166 kb on + strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.746 kb on - strand, within RR42_RS00300 at 86.769 kb on + strand, within RR42_RS00300 at 86.769 kb on + strand, within RR42_RS00300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 83,986 + RR42_RS00290 0.57 -0.3 83,986 + RR42_RS00290 0.57 +0.4 83,987 - RR42_RS00290 0.57 +1.3 83,987 - RR42_RS00290 0.57 +0.4 83,987 - RR42_RS00290 0.57 +0.5 84,322 + RR42_RS00290 0.86 -1.0 84,334 + RR42_RS00290 0.87 +0.5 84,334 + RR42_RS00290 0.87 -0.3 84,335 - RR42_RS00290 0.87 +0.9 84,463 + +0.4 84,463 + +0.8 84,464 - +0.5 84,505 + -0.7 84,506 - -1.3 84,576 + -0.4 84,997 + RR42_RS00295 0.29 -0.6 84,997 + RR42_RS00295 0.29 -0.8 84,998 - RR42_RS00295 0.29 -1.3 85,130 + RR42_RS00295 0.38 -0.5 85,130 + RR42_RS00295 0.38 +0.1 85,131 - RR42_RS00295 0.38 -0.5 85,131 - RR42_RS00295 0.38 +0.1 85,131 - RR42_RS00295 0.38 -0.7 85,598 + RR42_RS00295 0.71 -3.5 85,598 + RR42_RS00295 0.71 -1.7 85,598 + RR42_RS00295 0.71 -0.3 85,651 + RR42_RS00295 0.75 +0.3 85,691 - RR42_RS00295 0.78 -1.4 85,859 + RR42_RS00295 0.89 -3.3 85,907 + -1.4 85,907 + -0.9 85,907 + -2.0 85,908 - -0.7 86,166 + RR42_RS00300 0.11 +0.8 86,166 + RR42_RS00300 0.11 -0.2 86,167 - RR42_RS00300 0.11 +0.9 86,167 - RR42_RS00300 0.11 -0.4 86,167 - RR42_RS00300 0.11 -0.7 86,746 - RR42_RS00300 0.79 -2.2 86,769 + RR42_RS00300 0.82 -1.0 86,769 + RR42_RS00300 0.82 -0.9
Or see this region's nucleotide sequence