Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS12295 and LRK54_RS12300 are separated by 15 nucleotides LRK54_RS12300 and LRK54_RS12305 are separated by 69 nucleotides LRK54_RS12305 and LRK54_RS12310 overlap by 4 nucleotides
LRK54_RS12295: LRK54_RS12295 - symmetrical bis(5'-nucleosyl)-tetraphosphatase, at 2,735,704 to 2,736,555
_RS12295
LRK54_RS12300: LRK54_RS12300 - Co2+/Mg2+ efflux protein ApaG, at 2,736,571 to 2,736,954
_RS12300
LRK54_RS12305: LRK54_RS12305 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA, at 2,737,024 to 2,737,812
_RS12305
LRK54_RS12310: LRK54_RS12310 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 2,737,809 to 2,738,858
_RS12310
Position (kb)
2737
2738 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2736.050 kb on - strand, within LRK54_RS12295 at 2736.283 kb on + strand, within LRK54_RS12295 at 2736.283 kb on + strand, within LRK54_RS12295 at 2736.283 kb on + strand, within LRK54_RS12295 at 2736.283 kb on + strand, within LRK54_RS12295 at 2736.283 kb on + strand, within LRK54_RS12295 at 2736.283 kb on + strand, within LRK54_RS12295 at 2736.283 kb on + strand, within LRK54_RS12295 at 2736.283 kb on + strand, within LRK54_RS12295 at 2736.284 kb on - strand, within LRK54_RS12295 at 2736.284 kb on - strand, within LRK54_RS12295 at 2736.284 kb on - strand, within LRK54_RS12295 at 2736.284 kb on - strand, within LRK54_RS12295 at 2736.545 kb on + strand at 2736.545 kb on + strand at 2736.545 kb on + strand at 2736.546 kb on - strand at 2736.546 kb on - strand at 2736.549 kb on + strand at 2736.549 kb on + strand at 2736.549 kb on + strand at 2736.549 kb on + strand at 2736.549 kb on + strand at 2736.549 kb on + strand at 2736.549 kb on + strand at 2736.549 kb on + strand at 2736.550 kb on - strand at 2736.550 kb on - strand at 2736.550 kb on - strand at 2736.550 kb on - strand at 2736.550 kb on - strand at 2736.550 kb on - strand at 2736.583 kb on - strand at 2736.583 kb on - strand at 2736.653 kb on + strand, within LRK54_RS12300 at 2736.653 kb on + strand, within LRK54_RS12300 at 2736.654 kb on - strand, within LRK54_RS12300 at 2736.654 kb on - strand, within LRK54_RS12300 at 2736.654 kb on - strand, within LRK54_RS12300 at 2736.654 kb on - strand, within LRK54_RS12300 at 2736.654 kb on - strand, within LRK54_RS12300 at 2736.654 kb on - strand, within LRK54_RS12300 at 2736.654 kb on - strand, within LRK54_RS12300 at 2736.704 kb on + strand, within LRK54_RS12300 at 2736.704 kb on + strand, within LRK54_RS12300 at 2736.704 kb on + strand, within LRK54_RS12300 at 2736.704 kb on + strand, within LRK54_RS12300 at 2736.704 kb on + strand, within LRK54_RS12300 at 2736.705 kb on - strand, within LRK54_RS12300 at 2736.705 kb on - strand, within LRK54_RS12300 at 2736.705 kb on - strand, within LRK54_RS12300 at 2736.705 kb on - strand, within LRK54_RS12300 at 2736.705 kb on - strand, within LRK54_RS12300 at 2736.850 kb on - strand, within LRK54_RS12300 at 2736.858 kb on - strand, within LRK54_RS12300 at 2736.858 kb on - strand, within LRK54_RS12300 at 2736.869 kb on + strand, within LRK54_RS12300 at 2736.869 kb on + strand, within LRK54_RS12300 at 2736.869 kb on + strand, within LRK54_RS12300 at 2736.870 kb on - strand, within LRK54_RS12300 at 2736.870 kb on - strand, within LRK54_RS12300 at 2736.870 kb on - strand, within LRK54_RS12300 at 2736.870 kb on - strand, within LRK54_RS12300 at 2736.935 kb on + strand at 2736.935 kb on + strand at 2736.935 kb on + strand at 2736.935 kb on + strand at 2736.936 kb on - strand at 2736.936 kb on - strand at 2736.936 kb on - strand at 2736.936 kb on - strand at 2736.936 kb on - strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.965 kb on + strand at 2736.966 kb on - strand at 2736.966 kb on - strand at 2736.966 kb on - strand at 2736.966 kb on - strand at 2736.966 kb on - strand at 2736.966 kb on - strand at 2736.966 kb on - strand at 2736.966 kb on - strand at 2736.966 kb on - strand at 2737.037 kb on + strand at 2737.037 kb on + strand at 2737.038 kb on - strand at 2737.038 kb on - strand at 2737.038 kb on - strand at 2737.038 kb on - strand at 2737.358 kb on + strand, within LRK54_RS12305 at 2737.358 kb on + strand, within LRK54_RS12305 at 2737.358 kb on + strand, within LRK54_RS12305 at 2737.358 kb on + strand, within LRK54_RS12305 at 2737.358 kb on + strand, within LRK54_RS12305 at 2737.358 kb on + strand, within LRK54_RS12305 at 2737.359 kb on - strand, within LRK54_RS12305 at 2737.359 kb on - strand, within LRK54_RS12305 at 2737.359 kb on - strand, within LRK54_RS12305 at 2737.359 kb on - strand, within LRK54_RS12305 at 2737.359 kb on - strand, within LRK54_RS12305 at 2737.359 kb on - strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.478 kb on + strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.479 kb on - strand, within LRK54_RS12305 at 2737.481 kb on + strand, within LRK54_RS12305 at 2737.481 kb on + strand, within LRK54_RS12305 at 2737.481 kb on + strand, within LRK54_RS12305 at 2737.481 kb on + strand, within LRK54_RS12305 at 2737.481 kb on + strand, within LRK54_RS12305 at 2737.481 kb on + strand, within LRK54_RS12305 at 2737.481 kb on + strand, within LRK54_RS12305 at 2737.481 kb on + strand, within LRK54_RS12305 at 2737.482 kb on - strand, within LRK54_RS12305 at 2737.482 kb on - strand, within LRK54_RS12305 at 2737.482 kb on - strand, within LRK54_RS12305 at 2737.482 kb on - strand, within LRK54_RS12305 at 2737.482 kb on - strand, within LRK54_RS12305 at 2737.482 kb on - strand, within LRK54_RS12305 at 2737.757 kb on + strand at 2737.757 kb on + strand at 2737.757 kb on + strand at 2737.757 kb on + strand at 2737.757 kb on + strand at 2737.757 kb on + strand at 2737.758 kb on - strand at 2737.758 kb on - strand at 2737.758 kb on - strand at 2737.758 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 2,736,050 - LRK54_RS12295 0.41 -1.1 2,736,283 + LRK54_RS12295 0.68 +0.1 2,736,283 + LRK54_RS12295 0.68 -1.2 2,736,283 + LRK54_RS12295 0.68 -1.0 2,736,283 + LRK54_RS12295 0.68 -0.8 2,736,283 + LRK54_RS12295 0.68 -1.3 2,736,283 + LRK54_RS12295 0.68 -0.1 2,736,283 + LRK54_RS12295 0.68 -1.0 2,736,283 + LRK54_RS12295 0.68 -0.1 2,736,284 - LRK54_RS12295 0.68 -2.3 2,736,284 - LRK54_RS12295 0.68 -0.5 2,736,284 - LRK54_RS12295 0.68 -2.1 2,736,284 - LRK54_RS12295 0.68 -1.6 2,736,545 + -0.8 2,736,545 + +1.1 2,736,545 + -0.6 2,736,546 - -0.3 2,736,546 - -1.6 2,736,549 + +1.1 2,736,549 + -0.5 2,736,549 + -1.0 2,736,549 + -1.2 2,736,549 + -0.3 2,736,549 + -0.3 2,736,549 + -2.4 2,736,549 + -1.3 2,736,550 - -0.3 2,736,550 - +0.1 2,736,550 - -1.8 2,736,550 - -1.0 2,736,550 - -0.5 2,736,550 - -1.1 2,736,583 - -1.6 2,736,583 - +0.2 2,736,653 + LRK54_RS12300 0.21 +0.1 2,736,653 + LRK54_RS12300 0.21 -1.6 2,736,654 - LRK54_RS12300 0.22 -0.0 2,736,654 - LRK54_RS12300 0.22 -1.0 2,736,654 - LRK54_RS12300 0.22 -1.5 2,736,654 - LRK54_RS12300 0.22 -1.8 2,736,654 - LRK54_RS12300 0.22 +0.1 2,736,654 - LRK54_RS12300 0.22 -1.4 2,736,654 - LRK54_RS12300 0.22 +0.1 2,736,704 + LRK54_RS12300 0.35 +0.1 2,736,704 + LRK54_RS12300 0.35 -0.6 2,736,704 + LRK54_RS12300 0.35 +0.2 2,736,704 + LRK54_RS12300 0.35 -0.3 2,736,704 + LRK54_RS12300 0.35 -2.4 2,736,705 - LRK54_RS12300 0.35 -1.3 2,736,705 - LRK54_RS12300 0.35 -0.8 2,736,705 - LRK54_RS12300 0.35 -1.2 2,736,705 - LRK54_RS12300 0.35 +1.4 2,736,705 - LRK54_RS12300 0.35 -2.0 2,736,850 - LRK54_RS12300 0.73 +0.1 2,736,858 - LRK54_RS12300 0.75 -0.3 2,736,858 - LRK54_RS12300 0.75 +1.1 2,736,869 + LRK54_RS12300 0.78 -1.4 2,736,869 + LRK54_RS12300 0.78 -1.5 2,736,869 + LRK54_RS12300 0.78 -0.6 2,736,870 - LRK54_RS12300 0.78 -0.7 2,736,870 - LRK54_RS12300 0.78 -3.6 2,736,870 - LRK54_RS12300 0.78 -2.6 2,736,870 - LRK54_RS12300 0.78 +0.5 2,736,935 + +0.4 2,736,935 + +0.7 2,736,935 + -0.8 2,736,935 + -1.0 2,736,936 - -1.6 2,736,936 - +0.2 2,736,936 - -0.4 2,736,936 - -0.8 2,736,936 - -1.6 2,736,965 + -0.0 2,736,965 + +1.2 2,736,965 + -0.2 2,736,965 + -0.3 2,736,965 + -0.2 2,736,965 + +0.2 2,736,965 + -0.7 2,736,965 + -0.2 2,736,965 + -0.1 2,736,965 + +0.0 2,736,965 + +0.4 2,736,965 + +0.3 2,736,965 + +0.1 2,736,965 + +0.5 2,736,965 + +1.7 2,736,965 + +0.5 2,736,965 + -2.0 2,736,965 + -0.2 2,736,965 + -0.3 2,736,965 + -0.2 2,736,965 + +0.4 2,736,965 + +0.9 2,736,965 + +0.3 2,736,965 + +0.1 2,736,965 + -0.5 2,736,965 + +0.5 2,736,966 - +0.2 2,736,966 - +0.2 2,736,966 - +0.9 2,736,966 - +0.4 2,736,966 - +0.8 2,736,966 - +0.1 2,736,966 - -0.9 2,736,966 - +0.5 2,736,966 - +0.5 2,737,037 + -0.8 2,737,037 + -3.3 2,737,038 - +0.9 2,737,038 - -1.0 2,737,038 - +0.2 2,737,038 - -0.0 2,737,358 + LRK54_RS12305 0.42 -1.0 2,737,358 + LRK54_RS12305 0.42 -0.3 2,737,358 + LRK54_RS12305 0.42 -2.2 2,737,358 + LRK54_RS12305 0.42 +0.7 2,737,358 + LRK54_RS12305 0.42 -1.5 2,737,358 + LRK54_RS12305 0.42 -2.7 2,737,359 - LRK54_RS12305 0.42 -1.0 2,737,359 - LRK54_RS12305 0.42 -0.3 2,737,359 - LRK54_RS12305 0.42 -1.1 2,737,359 - LRK54_RS12305 0.42 -0.9 2,737,359 - LRK54_RS12305 0.42 -1.9 2,737,359 - LRK54_RS12305 0.42 -0.9 2,737,478 + LRK54_RS12305 0.58 -1.9 2,737,478 + LRK54_RS12305 0.58 -0.3 2,737,478 + LRK54_RS12305 0.58 -1.7 2,737,478 + LRK54_RS12305 0.58 -0.9 2,737,478 + LRK54_RS12305 0.58 -1.7 2,737,478 + LRK54_RS12305 0.58 -0.5 2,737,478 + LRK54_RS12305 0.58 -2.7 2,737,478 + LRK54_RS12305 0.58 -1.0 2,737,478 + LRK54_RS12305 0.58 -2.6 2,737,478 + LRK54_RS12305 0.58 -1.4 2,737,478 + LRK54_RS12305 0.58 -2.6 2,737,478 + LRK54_RS12305 0.58 -0.9 2,737,478 + LRK54_RS12305 0.58 -3.0 2,737,478 + LRK54_RS12305 0.58 -1.1 2,737,478 + LRK54_RS12305 0.58 -0.7 2,737,478 + LRK54_RS12305 0.58 -0.6 2,737,478 + LRK54_RS12305 0.58 -1.5 2,737,478 + LRK54_RS12305 0.58 -1.4 2,737,478 + LRK54_RS12305 0.58 -1.3 2,737,478 + LRK54_RS12305 0.58 -1.6 2,737,478 + LRK54_RS12305 0.58 -1.3 2,737,478 + -0.4 2,737,478 + LRK54_RS12305 0.58 -0.7 2,737,478 + LRK54_RS12305 0.58 -2.3 2,737,478 + LRK54_RS12305 0.58 -1.6 2,737,478 + LRK54_RS12305 0.58 -0.9 2,737,478 + LRK54_RS12305 0.58 -1.3 2,737,478 + LRK54_RS12305 0.58 -0.8 2,737,478 + LRK54_RS12305 0.58 +1.1 2,737,478 + LRK54_RS12305 0.58 -2.1 2,737,478 + LRK54_RS12305 0.58 -1.5 2,737,478 + LRK54_RS12305 0.58 -2.5 2,737,478 + LRK54_RS12305 0.58 -0.8 2,737,478 + LRK54_RS12305 0.58 -1.6 2,737,479 - LRK54_RS12305 0.58 -1.3 2,737,479 - LRK54_RS12305 0.58 -2.6 2,737,479 - LRK54_RS12305 0.58 -1.5 2,737,479 - LRK54_RS12305 0.58 -1.4 2,737,479 - LRK54_RS12305 0.58 -1.9 2,737,479 - LRK54_RS12305 0.58 -2.6 2,737,479 - LRK54_RS12305 0.58 -1.1 2,737,479 - LRK54_RS12305 0.58 -0.9 2,737,479 - LRK54_RS12305 0.58 -0.5 2,737,479 - LRK54_RS12305 0.58 -1.2 2,737,479 - LRK54_RS12305 0.58 -1.3 2,737,479 - LRK54_RS12305 0.58 -1.6 2,737,479 - LRK54_RS12305 0.58 -1.7 2,737,479 - LRK54_RS12305 0.58 -1.2 2,737,479 - LRK54_RS12305 0.58 -2.0 2,737,479 - LRK54_RS12305 0.58 -0.3 2,737,479 - LRK54_RS12305 0.58 -2.2 2,737,479 - LRK54_RS12305 0.58 -2.1 2,737,479 - LRK54_RS12305 0.58 -1.8 2,737,479 - LRK54_RS12305 0.58 -0.2 2,737,479 - LRK54_RS12305 0.58 -1.3 2,737,479 - LRK54_RS12305 0.58 -0.7 2,737,479 - LRK54_RS12305 0.58 -0.9 2,737,479 - LRK54_RS12305 0.58 -1.4 2,737,479 - LRK54_RS12305 0.58 -0.2 2,737,479 - LRK54_RS12305 0.58 -0.9 2,737,479 - LRK54_RS12305 0.58 -0.5 2,737,479 - LRK54_RS12305 0.58 -2.9 2,737,479 - LRK54_RS12305 0.58 -1.3 2,737,479 - LRK54_RS12305 0.58 -2.6 2,737,479 - LRK54_RS12305 0.58 -0.5 2,737,479 - LRK54_RS12305 0.58 -2.5 2,737,479 - LRK54_RS12305 0.58 -1.8 2,737,479 - LRK54_RS12305 0.58 -2.4 2,737,479 - LRK54_RS12305 0.58 -2.7 2,737,479 - LRK54_RS12305 0.58 -0.6 2,737,479 - LRK54_RS12305 0.58 -2.0 2,737,479 - LRK54_RS12305 0.58 -3.8 2,737,479 - LRK54_RS12305 0.58 +0.4 2,737,479 - LRK54_RS12305 0.58 -1.2 2,737,479 - LRK54_RS12305 0.58 -1.9 2,737,479 - LRK54_RS12305 0.58 -0.6 2,737,479 - LRK54_RS12305 0.58 -1.0 2,737,479 - LRK54_RS12305 0.58 -1.4 2,737,479 - LRK54_RS12305 0.58 -1.3 2,737,479 - LRK54_RS12305 0.58 -1.3 2,737,479 - LRK54_RS12305 0.58 -2.3 2,737,481 + LRK54_RS12305 0.58 -2.0 2,737,481 + LRK54_RS12305 0.58 +0.1 2,737,481 + LRK54_RS12305 0.58 -1.2 2,737,481 + LRK54_RS12305 0.58 -1.3 2,737,481 + LRK54_RS12305 0.58 -3.2 2,737,481 + LRK54_RS12305 0.58 -1.0 2,737,481 + LRK54_RS12305 0.58 -1.0 2,737,481 + LRK54_RS12305 0.58 -1.5 2,737,482 - LRK54_RS12305 0.58 -1.6 2,737,482 - LRK54_RS12305 0.58 -0.6 2,737,482 - LRK54_RS12305 0.58 -0.1 2,737,482 - LRK54_RS12305 0.58 -0.9 2,737,482 - LRK54_RS12305 0.58 -0.8 2,737,482 - LRK54_RS12305 0.58 -1.2 2,737,757 + -0.5 2,737,757 + -2.3 2,737,757 + -0.9 2,737,757 + +0.2 2,737,757 + -2.4 2,737,757 + -1.0 2,737,758 - -1.3 2,737,758 - -3.2 2,737,758 - -1.1 2,737,758 - -0.9
Or see this region's nucleotide sequence