Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25445 and CA265_RS25450 are separated by 163 nucleotides CA265_RS25450 and CA265_RS25455 are separated by 92 nucleotides CA265_RS25455 and CA265_RS25460 are separated by 162 nucleotides
CA265_RS25445: CA265_RS25445 - ABC transporter, at 6,177,434 to 6,178,663
_RS25445
CA265_RS25450: CA265_RS25450 - iron-sulfur cluster assembly accessory protein, at 6,178,827 to 6,179,180
_RS25450
CA265_RS25455: CA265_RS25455 - hypothetical protein, at 6,179,273 to 6,179,803
_RS25455
CA265_RS25460: CA265_RS25460 - methylmalonyl-CoA mutase, at 6,179,966 to 6,181,513
_RS25460
Position (kb)
6178
6179
6180 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6177.896 kb on + strand, within CA265_RS25445 at 6177.962 kb on - strand, within CA265_RS25445 at 6177.985 kb on - strand, within CA265_RS25445 at 6178.036 kb on - strand, within CA265_RS25445 at 6178.085 kb on + strand, within CA265_RS25445 at 6178.085 kb on + strand, within CA265_RS25445 at 6178.086 kb on - strand, within CA265_RS25445 at 6178.116 kb on - strand, within CA265_RS25445 at 6178.220 kb on - strand, within CA265_RS25445 at 6178.274 kb on + strand, within CA265_RS25445 at 6178.318 kb on - strand, within CA265_RS25445 at 6178.387 kb on + strand, within CA265_RS25445 at 6178.439 kb on - strand, within CA265_RS25445 at 6178.453 kb on + strand, within CA265_RS25445 at 6178.480 kb on + strand, within CA265_RS25445 at 6178.529 kb on + strand, within CA265_RS25445 at 6178.583 kb on - strand at 6178.600 kb on + strand at 6178.600 kb on + strand at 6178.601 kb on - strand at 6178.604 kb on - strand at 6178.624 kb on + strand at 6178.657 kb on - strand at 6178.675 kb on - strand at 6178.694 kb on - strand at 6178.707 kb on + strand at 6178.726 kb on + strand at 6178.726 kb on + strand at 6178.728 kb on + strand at 6178.729 kb on - strand at 6178.729 kb on - strand at 6178.734 kb on - strand at 6178.735 kb on + strand at 6178.805 kb on - strand at 6178.806 kb on + strand at 6178.806 kb on + strand at 6178.807 kb on - strand at 6178.807 kb on - strand at 6178.812 kb on - strand at 6178.812 kb on - strand at 6178.817 kb on - strand at 6178.820 kb on - strand at 6178.824 kb on + strand at 6178.825 kb on - strand at 6179.065 kb on - strand, within CA265_RS25450 at 6179.111 kb on + strand, within CA265_RS25450 at 6179.111 kb on + strand, within CA265_RS25450 at 6179.213 kb on + strand at 6179.272 kb on + strand at 6179.292 kb on + strand at 6179.299 kb on + strand at 6179.299 kb on + strand at 6179.301 kb on + strand at 6179.301 kb on + strand at 6179.355 kb on + strand, within CA265_RS25455 at 6179.356 kb on - strand, within CA265_RS25455 at 6179.385 kb on + strand, within CA265_RS25455 at 6179.385 kb on + strand, within CA265_RS25455 at 6179.422 kb on + strand, within CA265_RS25455 at 6179.433 kb on + strand, within CA265_RS25455 at 6179.434 kb on - strand, within CA265_RS25455 at 6179.464 kb on - strand, within CA265_RS25455 at 6179.489 kb on - strand, within CA265_RS25455 at 6179.489 kb on - strand, within CA265_RS25455 at 6179.535 kb on + strand, within CA265_RS25455 at 6179.545 kb on + strand, within CA265_RS25455 at 6179.582 kb on - strand, within CA265_RS25455 at 6179.601 kb on + strand, within CA265_RS25455 at 6179.645 kb on - strand, within CA265_RS25455 at 6179.660 kb on - strand, within CA265_RS25455 at 6179.670 kb on + strand, within CA265_RS25455 at 6179.773 kb on + strand at 6179.791 kb on + strand at 6179.792 kb on - strand at 6179.792 kb on - strand at 6179.891 kb on + strand at 6179.941 kb on + strand at 6179.942 kb on - strand at 6179.958 kb on - strand at 6179.958 kb on - strand at 6179.962 kb on + strand at 6180.085 kb on + strand at 6180.086 kb on - strand at 6180.089 kb on + strand at 6180.090 kb on - strand at 6180.119 kb on + strand at 6180.119 kb on + strand at 6180.165 kb on - strand, within CA265_RS25460 at 6180.165 kb on - strand, within CA265_RS25460 at 6180.169 kb on - strand, within CA265_RS25460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 6,177,896 + CA265_RS25445 0.38 +0.3 6,177,962 - CA265_RS25445 0.43 +0.6 6,177,985 - CA265_RS25445 0.45 -0.2 6,178,036 - CA265_RS25445 0.49 -0.4 6,178,085 + CA265_RS25445 0.53 -0.7 6,178,085 + CA265_RS25445 0.53 +0.5 6,178,086 - CA265_RS25445 0.53 +0.1 6,178,116 - CA265_RS25445 0.55 +0.3 6,178,220 - CA265_RS25445 0.64 +0.4 6,178,274 + CA265_RS25445 0.68 -0.1 6,178,318 - CA265_RS25445 0.72 +1.4 6,178,387 + CA265_RS25445 0.77 +0.2 6,178,439 - CA265_RS25445 0.82 -0.1 6,178,453 + CA265_RS25445 0.83 +2.0 6,178,480 + CA265_RS25445 0.85 -0.6 6,178,529 + CA265_RS25445 0.89 +0.3 6,178,583 - -0.8 6,178,600 + +0.5 6,178,600 + +0.3 6,178,601 - -1.1 6,178,604 - -0.6 6,178,624 + +0.9 6,178,657 - +0.0 6,178,675 - +0.5 6,178,694 - +0.5 6,178,707 + -0.9 6,178,726 + -0.4 6,178,726 + -0.3 6,178,728 + +0.2 6,178,729 - +0.6 6,178,729 - +0.1 6,178,734 - -0.7 6,178,735 + -0.7 6,178,805 - +0.6 6,178,806 + +0.2 6,178,806 + +0.3 6,178,807 - -0.7 6,178,807 - +0.8 6,178,812 - -0.2 6,178,812 - -0.5 6,178,817 - -0.1 6,178,820 - -0.0 6,178,824 + +0.5 6,178,825 - -0.4 6,179,065 - CA265_RS25450 0.67 -2.5 6,179,111 + CA265_RS25450 0.80 -1.4 6,179,111 + CA265_RS25450 0.80 -1.6 6,179,213 + +0.1 6,179,272 + -0.2 6,179,292 + -1.1 6,179,299 + +1.1 6,179,299 + -0.0 6,179,301 + +1.0 6,179,301 + +0.1 6,179,355 + CA265_RS25455 0.15 -0.3 6,179,356 - CA265_RS25455 0.16 +0.6 6,179,385 + CA265_RS25455 0.21 -0.9 6,179,385 + CA265_RS25455 0.21 -0.3 6,179,422 + CA265_RS25455 0.28 +0.1 6,179,433 + CA265_RS25455 0.30 -0.5 6,179,434 - CA265_RS25455 0.30 -0.4 6,179,464 - CA265_RS25455 0.36 +0.3 6,179,489 - CA265_RS25455 0.41 -2.3 6,179,489 - CA265_RS25455 0.41 +2.3 6,179,535 + CA265_RS25455 0.49 +0.6 6,179,545 + CA265_RS25455 0.51 +0.1 6,179,582 - CA265_RS25455 0.58 -0.3 6,179,601 + CA265_RS25455 0.62 +1.7 6,179,645 - CA265_RS25455 0.70 -2.6 6,179,660 - CA265_RS25455 0.73 -0.8 6,179,670 + CA265_RS25455 0.75 -1.8 6,179,773 + +0.3 6,179,791 + +0.2 6,179,792 - -1.5 6,179,792 - -0.4 6,179,891 + +1.7 6,179,941 + -1.3 6,179,942 - -0.9 6,179,958 - -2.3 6,179,958 - -0.2 6,179,962 + +0.7 6,180,085 + +0.7 6,180,086 - +0.3 6,180,089 + -0.9 6,180,090 - -0.5 6,180,119 + -0.9 6,180,119 + +0.4 6,180,165 - CA265_RS25460 0.13 -0.3 6,180,165 - CA265_RS25460 0.13 +0.4 6,180,169 - CA265_RS25460 0.13 +0.2
Or see this region's nucleotide sequence