Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS24725 and CA265_RS24730 are separated by 226 nucleotides CA265_RS24730 and CA265_RS24735 are separated by 70 nucleotides CA265_RS24735 and CA265_RS24740 are separated by 224 nucleotides
CA265_RS24725: CA265_RS24725 - multidrug transporter, at 6,037,484 to 6,040,510
_RS24725
CA265_RS24730: CA265_RS24730 - tRNA-Gln, at 6,040,737 to 6,040,810
_RS24730
CA265_RS24735: CA265_RS24735 - ribose-phosphate pyrophosphokinase, at 6,040,881 to 6,041,822
_RS24735
CA265_RS24740: CA265_RS24740 - 50S ribosomal protein L25/general stress protein Ctc, at 6,042,047 to 6,042,631
_RS24740
Position (kb)
6040
6041
6042 Strain fitness (log2 ratio)
-1
0
1
2 at 6039.905 kb on + strand, within CA265_RS24725 at 6039.922 kb on + strand, within CA265_RS24725 at 6039.967 kb on - strand, within CA265_RS24725 at 6039.967 kb on - strand, within CA265_RS24725 at 6040.041 kb on - strand, within CA265_RS24725 at 6040.072 kb on - strand, within CA265_RS24725 at 6040.085 kb on + strand, within CA265_RS24725 at 6040.086 kb on - strand, within CA265_RS24725 at 6040.125 kb on - strand, within CA265_RS24725 at 6040.125 kb on - strand, within CA265_RS24725 at 6040.219 kb on + strand at 6040.274 kb on - strand at 6040.284 kb on + strand at 6040.289 kb on + strand at 6040.503 kb on + strand at 6040.522 kb on - strand at 6041.820 kb on + strand at 6041.820 kb on + strand at 6041.852 kb on + strand at 6041.874 kb on + strand at 6041.874 kb on + strand at 6041.894 kb on + strand at 6041.944 kb on + strand at 6041.950 kb on + strand at 6042.697 kb on + strand at 6042.768 kb on - strand at 6042.777 kb on + strand at 6042.779 kb on + strand at 6042.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 6,039,905 + CA265_RS24725 0.80 +0.3 6,039,922 + CA265_RS24725 0.81 -0.2 6,039,967 - CA265_RS24725 0.82 +0.3 6,039,967 - CA265_RS24725 0.82 -1.5 6,040,041 - CA265_RS24725 0.84 -0.3 6,040,072 - CA265_RS24725 0.85 -0.8 6,040,085 + CA265_RS24725 0.86 +1.1 6,040,086 - CA265_RS24725 0.86 +0.2 6,040,125 - CA265_RS24725 0.87 -0.4 6,040,125 - CA265_RS24725 0.87 +0.5 6,040,219 + +1.4 6,040,274 - +0.9 6,040,284 + +0.1 6,040,289 + +2.4 6,040,503 + -1.0 6,040,522 - -0.3 6,041,820 + +0.5 6,041,820 + +0.3 6,041,852 + -1.7 6,041,874 + +0.8 6,041,874 + +1.7 6,041,894 + -1.0 6,041,944 + +1.0 6,041,950 + -0.1 6,042,697 + +0.7 6,042,768 - -1.1 6,042,777 + -0.6 6,042,779 + +0.3 6,042,780 - +0.3
Or see this region's nucleotide sequence