Experiment: L-Phenylalanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28005 and BPHYT_RS28010 are separated by 78 nucleotides BPHYT_RS28010 and BPHYT_RS28015 are separated by 545 nucleotides BPHYT_RS28015 and BPHYT_RS28020 overlap by 4 nucleotides BPHYT_RS28020 and BPHYT_RS28025 overlap by 4 nucleotides
BPHYT_RS28005: BPHYT_RS28005 - hypothetical protein, at 1,847,442 to 1,847,684
_RS28005
BPHYT_RS28010: BPHYT_RS28010 - hypothetical protein, at 1,847,763 to 1,847,945
_RS28010
BPHYT_RS28015: BPHYT_RS28015 - enoyl-CoA hydratase, at 1,848,491 to 1,849,639
_RS28015
BPHYT_RS28020: BPHYT_RS28020 - enoyl-CoA hydratase, at 1,849,636 to 1,850,454
_RS28020
BPHYT_RS28025: BPHYT_RS28025 - 3-hydroxyisobutyrate dehydrogenase, at 1,850,451 to 1,851,365
_RS28025
Position (kb) 1848
1849
1850 Strain fitness (log2 ratio) -1
0
1 at 1848.227 kb on + strand at 1848.227 kb on + strand at 1848.228 kb on - strand at 1848.331 kb on + strand at 1848.756 kb on + strand, within BPHYT_RS28015 at 1848.793 kb on - strand, within BPHYT_RS28015 at 1848.793 kb on - strand, within BPHYT_RS28015 at 1849.117 kb on - strand, within BPHYT_RS28015 at 1850.438 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (C) remove 1,848,227 + -0.0 1,848,227 + +0.3 1,848,228 - -1.3 1,848,331 + +0.5 1,848,756 + BPHYT_RS28015 0.23 -0.1 1,848,793 - BPHYT_RS28015 0.26 -0.7 1,848,793 - BPHYT_RS28015 0.26 -0.7 1,849,117 - BPHYT_RS28015 0.54 +0.4 1,850,438 + -0.1
Or see this region's nucleotide sequence