Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5400

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5398 and Pf6N2E2_5399 are separated by 1 nucleotidesPf6N2E2_5399 and Pf6N2E2_5400 are separated by 137 nucleotidesPf6N2E2_5400 and Pf6N2E2_5401 are separated by 99 nucleotides Pf6N2E2_5398 - Ornithine cyclodeaminase (EC 4.3.1.12), at 6,203,710 to 6,203,892 _5398 Pf6N2E2_5399 - Ornithine cyclodeaminase (EC 4.3.1.12), at 6,203,894 to 6,204,661 _5399 Pf6N2E2_5400 - ATP-dependent RNA helicase RhlB, at 6,204,799 to 6,206,274 _5400 Pf6N2E2_5401 - phospholipase/carboxylesterase family protein, at 6,206,374 to 6,207,030 _5401 Position (kb) 6204 6205 6206 6207Strain fitness (log2 ratio) -2 -1 0 1 2at 6203.993 kb on - strand, within Pf6N2E2_5399at 6203.993 kb on - strand, within Pf6N2E2_5399at 6204.186 kb on + strand, within Pf6N2E2_5399at 6204.187 kb on - strand, within Pf6N2E2_5399at 6204.225 kb on + strand, within Pf6N2E2_5399at 6204.225 kb on + strand, within Pf6N2E2_5399at 6204.225 kb on + strand, within Pf6N2E2_5399at 6204.225 kb on + strand, within Pf6N2E2_5399at 6204.225 kb on + strand, within Pf6N2E2_5399at 6204.225 kb on + strand, within Pf6N2E2_5399at 6204.225 kb on + strand, within Pf6N2E2_5399at 6204.225 kb on + strand, within Pf6N2E2_5399at 6204.226 kb on - strand, within Pf6N2E2_5399at 6204.226 kb on - strand, within Pf6N2E2_5399at 6204.226 kb on - strand, within Pf6N2E2_5399at 6204.438 kb on + strand, within Pf6N2E2_5399at 6204.438 kb on + strand, within Pf6N2E2_5399at 6204.438 kb on + strand, within Pf6N2E2_5399at 6204.439 kb on - strand, within Pf6N2E2_5399at 6204.439 kb on - strand, within Pf6N2E2_5399at 6204.439 kb on - strand, within Pf6N2E2_5399at 6204.459 kb on + strand, within Pf6N2E2_5399at 6204.460 kb on - strand, within Pf6N2E2_5399at 6204.460 kb on - strand, within Pf6N2E2_5399at 6204.489 kb on + strand, within Pf6N2E2_5399at 6204.489 kb on + strand, within Pf6N2E2_5399at 6204.490 kb on - strand, within Pf6N2E2_5399at 6204.490 kb on - strand, within Pf6N2E2_5399at 6204.645 kb on + strandat 6204.645 kb on + strandat 6204.645 kb on + strandat 6204.646 kb on - strandat 6204.646 kb on - strandat 6204.646 kb on - strandat 6204.646 kb on - strandat 6204.646 kb on - strandat 6204.776 kb on + strandat 6204.776 kb on + strandat 6204.777 kb on - strandat 6204.800 kb on + strandat 6204.800 kb on + strandat 6204.801 kb on - strandat 6204.801 kb on - strandat 6204.801 kb on - strandat 6204.826 kb on + strandat 6204.827 kb on - strandat 6204.827 kb on - strandat 6204.827 kb on - strandat 6204.827 kb on - strandat 6204.974 kb on + strand, within Pf6N2E2_5400at 6204.974 kb on + strand, within Pf6N2E2_5400at 6204.975 kb on - strand, within Pf6N2E2_5400at 6204.975 kb on - strand, within Pf6N2E2_5400at 6205.250 kb on + strand, within Pf6N2E2_5400at 6205.250 kb on + strand, within Pf6N2E2_5400at 6205.250 kb on + strand, within Pf6N2E2_5400at 6205.250 kb on + strand, within Pf6N2E2_5400at 6205.250 kb on + strand, within Pf6N2E2_5400at 6205.262 kb on + strand, within Pf6N2E2_5400at 6205.262 kb on + strand, within Pf6N2E2_5400at 6205.262 kb on + strand, within Pf6N2E2_5400at 6205.262 kb on + strand, within Pf6N2E2_5400at 6205.262 kb on + strand, within Pf6N2E2_5400at 6205.263 kb on - strand, within Pf6N2E2_5400at 6205.263 kb on - strand, within Pf6N2E2_5400at 6205.263 kb on - strand, within Pf6N2E2_5400at 6205.263 kb on - strand, within Pf6N2E2_5400at 6205.286 kb on + strand, within Pf6N2E2_5400at 6205.286 kb on + strand, within Pf6N2E2_5400at 6205.286 kb on + strand, within Pf6N2E2_5400at 6205.286 kb on + strand, within Pf6N2E2_5400at 6205.286 kb on + strand, within Pf6N2E2_5400at 6205.286 kb on + strand, within Pf6N2E2_5400at 6205.287 kb on - strand, within Pf6N2E2_5400at 6205.287 kb on - strand, within Pf6N2E2_5400at 6205.287 kb on - strand, within Pf6N2E2_5400at 6205.287 kb on - strand, within Pf6N2E2_5400at 6205.287 kb on - strand, within Pf6N2E2_5400at 6205.287 kb on - strand, within Pf6N2E2_5400at 6205.287 kb on - strand, within Pf6N2E2_5400at 6205.287 kb on - strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.290 kb on + strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.291 kb on - strand, within Pf6N2E2_5400at 6205.667 kb on + strand, within Pf6N2E2_5400at 6205.667 kb on + strand, within Pf6N2E2_5400at 6205.705 kb on + strand, within Pf6N2E2_5400at 6205.754 kb on + strand, within Pf6N2E2_5400at 6205.754 kb on + strand, within Pf6N2E2_5400at 6205.754 kb on + strand, within Pf6N2E2_5400at 6205.755 kb on - strand, within Pf6N2E2_5400at 6205.755 kb on - strand, within Pf6N2E2_5400at 6205.755 kb on - strand, within Pf6N2E2_5400at 6205.755 kb on - strand, within Pf6N2E2_5400at 6205.755 kb on - strand, within Pf6N2E2_5400at 6205.755 kb on - strand, within Pf6N2E2_5400at 6205.871 kb on + strand, within Pf6N2E2_5400at 6205.990 kb on + strand, within Pf6N2E2_5400at 6205.990 kb on + strand, within Pf6N2E2_5400at 6205.991 kb on - strand, within Pf6N2E2_5400at 6205.991 kb on - strand, within Pf6N2E2_5400at 6206.297 kb on + strandat 6206.298 kb on - strandat 6206.312 kb on - strandat 6206.312 kb on - strandat 6206.425 kb on + strandat 6206.448 kb on - strand, within Pf6N2E2_5401at 6206.482 kb on + strand, within Pf6N2E2_5401at 6206.482 kb on + strand, within Pf6N2E2_5401at 6206.482 kb on + strand, within Pf6N2E2_5401at 6206.482 kb on + strand, within Pf6N2E2_5401at 6206.483 kb on - strand, within Pf6N2E2_5401at 6206.483 kb on - strand, within Pf6N2E2_5401at 6206.483 kb on - strand, within Pf6N2E2_5401at 6206.483 kb on - strand, within Pf6N2E2_5401at 6206.486 kb on + strand, within Pf6N2E2_5401at 6206.487 kb on - strand, within Pf6N2E2_5401at 6206.487 kb on - strand, within Pf6N2E2_5401at 6206.487 kb on - strand, within Pf6N2E2_5401at 6206.487 kb on - strand, within Pf6N2E2_5401at 6206.531 kb on + strand, within Pf6N2E2_5401at 6206.531 kb on + strand, within Pf6N2E2_5401at 6206.609 kb on + strand, within Pf6N2E2_5401at 6206.609 kb on + strand, within Pf6N2E2_5401at 6206.609 kb on + strand, within Pf6N2E2_5401at 6206.609 kb on + strand, within Pf6N2E2_5401at 6206.609 kb on + strand, within Pf6N2E2_5401at 6206.609 kb on + strand, within Pf6N2E2_5401at 6206.609 kb on + strand, within Pf6N2E2_5401at 6206.610 kb on - strand, within Pf6N2E2_5401at 6206.610 kb on - strand, within Pf6N2E2_5401at 6206.610 kb on - strand, within Pf6N2E2_5401at 6206.610 kb on - strand, within Pf6N2E2_5401at 6206.613 kb on + strand, within Pf6N2E2_5401at 6206.613 kb on + strand, within Pf6N2E2_5401at 6206.613 kb on + strand, within Pf6N2E2_5401at 6206.613 kb on + strand, within Pf6N2E2_5401at 6206.614 kb on - strand, within Pf6N2E2_5401at 6206.614 kb on - strand, within Pf6N2E2_5401at 6206.614 kb on - strand, within Pf6N2E2_5401at 6206.669 kb on + strand, within Pf6N2E2_5401at 6206.669 kb on + strand, within Pf6N2E2_5401at 6206.670 kb on - strand, within Pf6N2E2_5401at 6206.670 kb on - strand, within Pf6N2E2_5401at 6206.722 kb on + strand, within Pf6N2E2_5401at 6206.828 kb on + strand, within Pf6N2E2_5401at 6206.980 kb on - strandat 6207.076 kb on + strandat 6207.076 kb on + strandat 6207.076 kb on + strandat 6207.077 kb on - strandat 6207.101 kb on + strandat 6207.105 kb on + strandat 6207.105 kb on + strandat 6207.106 kb on - strandat 6207.106 kb on - strandat 6207.145 kb on + strandat 6207.192 kb on - strandat 6207.209 kb on + strandat 6207.210 kb on - strandat 6207.227 kb on + strandat 6207.227 kb on + strandat 6207.227 kb on + strandat 6207.227 kb on + strandat 6207.227 kb on + strandat 6207.228 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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6,203,993 - Pf6N2E2_5399 0.13 +0.8
6,203,993 - Pf6N2E2_5399 0.13 +0.5
6,204,186 + Pf6N2E2_5399 0.38 -0.7
6,204,187 - Pf6N2E2_5399 0.38 -0.2
6,204,225 + Pf6N2E2_5399 0.43 -0.1
6,204,225 + Pf6N2E2_5399 0.43 +0.2
6,204,225 + Pf6N2E2_5399 0.43 +0.8
6,204,225 + Pf6N2E2_5399 0.43 +0.2
6,204,225 + Pf6N2E2_5399 0.43 +0.7
6,204,225 + Pf6N2E2_5399 0.43 -0.6
6,204,225 + Pf6N2E2_5399 0.43 +0.5
6,204,225 + Pf6N2E2_5399 0.43 -0.8
6,204,226 - Pf6N2E2_5399 0.43 +0.5
6,204,226 - Pf6N2E2_5399 0.43 +0.2
6,204,226 - Pf6N2E2_5399 0.43 +0.6
6,204,438 + Pf6N2E2_5399 0.71 +0.5
6,204,438 + Pf6N2E2_5399 0.71 -0.4
6,204,438 + Pf6N2E2_5399 0.71 -0.7
6,204,439 - Pf6N2E2_5399 0.71 -0.3
6,204,439 - Pf6N2E2_5399 0.71 +0.5
6,204,439 - Pf6N2E2_5399 0.71 +0.1
6,204,459 + Pf6N2E2_5399 0.74 -0.2
6,204,460 - Pf6N2E2_5399 0.74 -0.3
6,204,460 - Pf6N2E2_5399 0.74 -0.5
6,204,489 + Pf6N2E2_5399 0.77 -0.7
6,204,489 + Pf6N2E2_5399 0.77 -0.4
6,204,490 - Pf6N2E2_5399 0.78 -0.4
6,204,490 - Pf6N2E2_5399 0.78 -1.4
6,204,645 + -0.3
6,204,645 + -0.2
6,204,645 + +0.0
6,204,646 - -1.0
6,204,646 - -0.0
6,204,646 - -0.7
6,204,646 - -0.5
6,204,646 - -0.4
6,204,776 + +0.1
6,204,776 + +0.4
6,204,777 - +0.4
6,204,800 + +0.4
6,204,800 + +0.3
6,204,801 - -0.4
6,204,801 - -0.4
6,204,801 - -0.2
6,204,826 + -1.5
6,204,827 - -0.2
6,204,827 - -0.2
6,204,827 - +0.6
6,204,827 - -0.3
6,204,974 + Pf6N2E2_5400 0.12 +0.4
6,204,974 + Pf6N2E2_5400 0.12 +1.0
6,204,975 - Pf6N2E2_5400 0.12 -0.0
6,204,975 - Pf6N2E2_5400 0.12 +0.4
6,205,250 + Pf6N2E2_5400 0.31 -0.1
6,205,250 + Pf6N2E2_5400 0.31 +0.4
6,205,250 + Pf6N2E2_5400 0.31 -0.9
6,205,250 + Pf6N2E2_5400 0.31 -0.7
6,205,250 + Pf6N2E2_5400 0.31 +0.2
6,205,262 + Pf6N2E2_5400 0.31 -0.2
6,205,262 + Pf6N2E2_5400 0.31 +0.2
6,205,262 + Pf6N2E2_5400 0.31 -0.6
6,205,262 + Pf6N2E2_5400 0.31 +0.2
6,205,262 + Pf6N2E2_5400 0.31 +0.1
6,205,263 - Pf6N2E2_5400 0.31 +0.9
6,205,263 - Pf6N2E2_5400 0.31 -0.4
6,205,263 - Pf6N2E2_5400 0.31 +0.0
6,205,263 - Pf6N2E2_5400 0.31 -0.5
6,205,286 + Pf6N2E2_5400 0.33 +1.1
6,205,286 + Pf6N2E2_5400 0.33 +0.1
6,205,286 + Pf6N2E2_5400 0.33 +0.7
6,205,286 + Pf6N2E2_5400 0.33 +0.1
6,205,286 + Pf6N2E2_5400 0.33 +0.5
6,205,286 + Pf6N2E2_5400 0.33 -0.6
6,205,287 - Pf6N2E2_5400 0.33 +0.1
6,205,287 - Pf6N2E2_5400 0.33 -1.6
6,205,287 - Pf6N2E2_5400 0.33 +0.8
6,205,287 - Pf6N2E2_5400 0.33 +0.0
6,205,287 - Pf6N2E2_5400 0.33 +0.2
6,205,287 - Pf6N2E2_5400 0.33 +0.6
6,205,287 - Pf6N2E2_5400 0.33 -0.2
6,205,287 - Pf6N2E2_5400 0.33 -1.3
6,205,290 + Pf6N2E2_5400 0.33 +0.8
6,205,290 + Pf6N2E2_5400 0.33 +0.2
6,205,290 + Pf6N2E2_5400 0.33 -0.3
6,205,290 + Pf6N2E2_5400 0.33 +1.1
6,205,290 + Pf6N2E2_5400 0.33 +0.3
6,205,290 + Pf6N2E2_5400 0.33 +0.1
6,205,290 + Pf6N2E2_5400 0.33 +0.3
6,205,290 + Pf6N2E2_5400 0.33 -0.2
6,205,290 + Pf6N2E2_5400 0.33 +0.7
6,205,290 + Pf6N2E2_5400 0.33 +1.1
6,205,290 + Pf6N2E2_5400 0.33 +1.0
6,205,290 + Pf6N2E2_5400 0.33 +0.8
6,205,291 - Pf6N2E2_5400 0.33 +0.0
6,205,291 - Pf6N2E2_5400 0.33 +0.6
6,205,291 - Pf6N2E2_5400 0.33 -1.1
6,205,291 - Pf6N2E2_5400 0.33 -1.5
6,205,291 - Pf6N2E2_5400 0.33 -0.1
6,205,291 - Pf6N2E2_5400 0.33 +0.8
6,205,291 - Pf6N2E2_5400 0.33 +0.2
6,205,291 - Pf6N2E2_5400 0.33 +0.4
6,205,291 - Pf6N2E2_5400 0.33 +0.2
6,205,291 - Pf6N2E2_5400 0.33 -0.3
6,205,291 - Pf6N2E2_5400 0.33 -1.0
6,205,291 - Pf6N2E2_5400 0.33 +0.6
6,205,291 - Pf6N2E2_5400 0.33 +0.2
6,205,291 - Pf6N2E2_5400 0.33 -0.2
6,205,291 - Pf6N2E2_5400 0.33 +0.0
6,205,291 - Pf6N2E2_5400 0.33 +2.1
6,205,291 - Pf6N2E2_5400 0.33 +0.0
6,205,291 - Pf6N2E2_5400 0.33 -0.1
6,205,291 - Pf6N2E2_5400 0.33 +0.8
6,205,291 - Pf6N2E2_5400 0.33 +0.0
6,205,291 - Pf6N2E2_5400 0.33 -0.7
6,205,291 - Pf6N2E2_5400 0.33 +0.8
6,205,667 + Pf6N2E2_5400 0.59 +0.6
6,205,667 + Pf6N2E2_5400 0.59 +0.6
6,205,705 + Pf6N2E2_5400 0.61 +0.2
6,205,754 + Pf6N2E2_5400 0.65 -0.4
6,205,754 + Pf6N2E2_5400 0.65 -0.3
6,205,754 + Pf6N2E2_5400 0.65 +0.5
6,205,755 - Pf6N2E2_5400 0.65 +0.1
6,205,755 - Pf6N2E2_5400 0.65 +0.9
6,205,755 - Pf6N2E2_5400 0.65 -0.4
6,205,755 - Pf6N2E2_5400 0.65 +0.5
6,205,755 - Pf6N2E2_5400 0.65 -0.9
6,205,755 - Pf6N2E2_5400 0.65 +0.2
6,205,871 + Pf6N2E2_5400 0.73 +0.3
6,205,990 + Pf6N2E2_5400 0.81 +0.4
6,205,990 + Pf6N2E2_5400 0.81 +0.6
6,205,991 - Pf6N2E2_5400 0.81 +0.1
6,205,991 - Pf6N2E2_5400 0.81 -0.3
6,206,297 + -0.2
6,206,298 - -0.5
6,206,312 - -1.5
6,206,312 - -0.2
6,206,425 + -1.2
6,206,448 - Pf6N2E2_5401 0.11 -0.5
6,206,482 + Pf6N2E2_5401 0.16 -0.4
6,206,482 + Pf6N2E2_5401 0.16 +0.2
6,206,482 + Pf6N2E2_5401 0.16 +0.4
6,206,482 + Pf6N2E2_5401 0.16 -0.5
6,206,483 - Pf6N2E2_5401 0.17 -0.4
6,206,483 - Pf6N2E2_5401 0.17 -1.7
6,206,483 - Pf6N2E2_5401 0.17 -0.2
6,206,483 - Pf6N2E2_5401 0.17 -0.5
6,206,486 + Pf6N2E2_5401 0.17 -0.2
6,206,487 - Pf6N2E2_5401 0.17 -0.9
6,206,487 - Pf6N2E2_5401 0.17 -0.5
6,206,487 - Pf6N2E2_5401 0.17 -1.1
6,206,487 - Pf6N2E2_5401 0.17 -0.5
6,206,531 + Pf6N2E2_5401 0.24 -0.0
6,206,531 + Pf6N2E2_5401 0.24 -0.3
6,206,609 + Pf6N2E2_5401 0.36 -0.9
6,206,609 + Pf6N2E2_5401 0.36 -1.5
6,206,609 + Pf6N2E2_5401 0.36 -0.1
6,206,609 + Pf6N2E2_5401 0.36 +0.3
6,206,609 + Pf6N2E2_5401 0.36 +0.1
6,206,609 + Pf6N2E2_5401 0.36 +0.8
6,206,609 + Pf6N2E2_5401 0.36 +0.3
6,206,610 - Pf6N2E2_5401 0.36 -0.5
6,206,610 - Pf6N2E2_5401 0.36 -1.7
6,206,610 - Pf6N2E2_5401 0.36 -0.9
6,206,610 - Pf6N2E2_5401 0.36 -1.5
6,206,613 + Pf6N2E2_5401 0.36 +1.9
6,206,613 + Pf6N2E2_5401 0.36 -0.3
6,206,613 + Pf6N2E2_5401 0.36 +0.6
6,206,613 + Pf6N2E2_5401 0.36 -0.2
6,206,614 - Pf6N2E2_5401 0.37 -0.6
6,206,614 - Pf6N2E2_5401 0.37 -1.0
6,206,614 - Pf6N2E2_5401 0.37 -2.7
6,206,669 + Pf6N2E2_5401 0.45 -0.2
6,206,669 + Pf6N2E2_5401 0.45 -2.2
6,206,670 - Pf6N2E2_5401 0.45 +0.4
6,206,670 - Pf6N2E2_5401 0.45 -0.5
6,206,722 + Pf6N2E2_5401 0.53 -0.3
6,206,828 + Pf6N2E2_5401 0.69 +0.6
6,206,980 - -1.1
6,207,076 + +0.6
6,207,076 + -0.3
6,207,076 + +0.1
6,207,077 - -1.0
6,207,101 + +0.3
6,207,105 + +0.5
6,207,105 + -0.4
6,207,106 - -0.6
6,207,106 - -0.2
6,207,145 + +0.3
6,207,192 - -0.0
6,207,209 + -0.1
6,207,210 - +0.7
6,207,227 + +0.6
6,207,227 + +0.8
6,207,227 + -0.3
6,207,227 + +0.8
6,207,227 + +0.1
6,207,228 - -0.2

Or see this region's nucleotide sequence