Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08215 and MMJJ_RS08220 are separated by 120 nucleotides MMJJ_RS08220 and MMJJ_RS08225 are separated by 190 nucleotides MMJJ_RS08225 and MMJJ_RS08230 are separated by 68 nucleotides
MMJJ_RS08215: MMJJ_RS08215 - radical SAM protein, at 1,512,599 to 1,513,714
_RS08215
MMJJ_RS08220: MMJJ_RS08220 - methenyltetrahydromethanopterin cyclohydrolase, at 1,513,835 to 1,514,806
_RS08220
MMJJ_RS08225: MMJJ_RS08225 - peptidylprolyl isomerase, at 1,514,997 to 1,515,449
_RS08225
MMJJ_RS08230: MMJJ_RS08230 - ribose-5-phosphate isomerase RpiA, at 1,515,518 to 1,516,237
_RS08230
Position (kb)
1513
1514
1515 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1512.850 kb on - strand, within MMJJ_RS08215 at 1512.860 kb on + strand, within MMJJ_RS08215 at 1512.870 kb on + strand, within MMJJ_RS08215 at 1512.991 kb on - strand, within MMJJ_RS08215 at 1512.991 kb on - strand, within MMJJ_RS08215 at 1512.992 kb on + strand, within MMJJ_RS08215 at 1512.992 kb on + strand, within MMJJ_RS08215 at 1513.001 kb on + strand, within MMJJ_RS08215 at 1513.017 kb on - strand, within MMJJ_RS08215 at 1513.018 kb on + strand, within MMJJ_RS08215 at 1513.058 kb on + strand, within MMJJ_RS08215 at 1513.098 kb on - strand, within MMJJ_RS08215 at 1513.098 kb on - strand, within MMJJ_RS08215 at 1513.098 kb on - strand, within MMJJ_RS08215 at 1513.233 kb on - strand, within MMJJ_RS08215 at 1513.274 kb on + strand, within MMJJ_RS08215 at 1513.298 kb on - strand, within MMJJ_RS08215 at 1513.298 kb on - strand, within MMJJ_RS08215 at 1513.299 kb on + strand, within MMJJ_RS08215 at 1513.344 kb on + strand, within MMJJ_RS08215 at 1513.386 kb on - strand, within MMJJ_RS08215 at 1513.434 kb on + strand, within MMJJ_RS08215 at 1513.437 kb on - strand, within MMJJ_RS08215 at 1513.437 kb on - strand, within MMJJ_RS08215 at 1513.460 kb on - strand, within MMJJ_RS08215 at 1513.481 kb on - strand, within MMJJ_RS08215 at 1513.495 kb on - strand, within MMJJ_RS08215 at 1513.509 kb on - strand, within MMJJ_RS08215 at 1513.509 kb on - strand, within MMJJ_RS08215 at 1513.509 kb on - strand, within MMJJ_RS08215 at 1513.602 kb on - strand, within MMJJ_RS08215 at 1513.605 kb on - strand at 1513.630 kb on - strand at 1513.631 kb on + strand at 1513.633 kb on - strand at 1513.633 kb on - strand at 1513.635 kb on - strand at 1513.635 kb on - strand at 1513.658 kb on - strand at 1513.658 kb on - strand at 1513.665 kb on - strand at 1513.771 kb on - strand at 1513.774 kb on - strand at 1513.775 kb on + strand at 1513.787 kb on + strand at 1513.803 kb on - strand at 1513.807 kb on - strand at 1513.807 kb on - strand at 1513.808 kb on + strand at 1513.808 kb on + strand at 1513.808 kb on + strand at 1513.809 kb on - strand at 1513.813 kb on - strand at 1513.814 kb on + strand at 1513.815 kb on - strand at 1513.820 kb on + strand at 1513.820 kb on + strand at 1513.822 kb on + strand at 1513.826 kb on - strand at 1513.826 kb on - strand at 1513.826 kb on - strand at 1513.827 kb on + strand at 1513.827 kb on + strand at 1513.827 kb on + strand at 1513.831 kb on - strand at 1513.837 kb on + strand at 1513.838 kb on - strand at 1513.839 kb on + strand at 1513.868 kb on - strand at 1513.869 kb on + strand at 1513.891 kb on + strand at 1513.991 kb on - strand, within MMJJ_RS08220 at 1514.681 kb on - strand at 1514.708 kb on + strand, within MMJJ_RS08220 at 1514.725 kb on - strand at 1514.730 kb on + strand at 1514.911 kb on + strand at 1514.911 kb on + strand at 1514.915 kb on - strand at 1514.915 kb on - strand at 1514.916 kb on + strand at 1514.916 kb on + strand at 1514.918 kb on + strand at 1514.918 kb on + strand at 1514.933 kb on - strand at 1514.933 kb on - strand at 1514.933 kb on - strand at 1514.938 kb on - strand at 1514.939 kb on + strand at 1514.939 kb on + strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.945 kb on + strand at 1514.945 kb on + strand at 1514.945 kb on + strand at 1514.947 kb on - strand at 1514.948 kb on + strand at 1514.948 kb on + strand at 1514.948 kb on + strand at 1514.982 kb on - strand at 1514.982 kb on - strand at 1515.014 kb on - strand at 1515.015 kb on + strand at 1515.020 kb on + strand at 1515.020 kb on + strand at 1515.030 kb on - strand at 1515.066 kb on + strand, within MMJJ_RS08225 at 1515.120 kb on - strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.131 kb on + strand, within MMJJ_RS08225 at 1515.131 kb on + strand, within MMJJ_RS08225 at 1515.138 kb on + strand, within MMJJ_RS08225 at 1515.355 kb on - strand, within MMJJ_RS08225 at 1515.362 kb on - strand, within MMJJ_RS08225 at 1515.435 kb on + strand at 1515.436 kb on - strand at 1515.437 kb on + strand at 1515.462 kb on + strand at 1515.470 kb on - strand at 1515.470 kb on - strand at 1515.489 kb on - strand at 1515.498 kb on + strand at 1515.498 kb on + strand at 1515.524 kb on + strand at 1515.550 kb on + strand at 1515.699 kb on + strand, within MMJJ_RS08230 at 1515.700 kb on - strand, within MMJJ_RS08230 at 1515.715 kb on - strand, within MMJJ_RS08230 at 1515.716 kb on + strand, within MMJJ_RS08230 at 1515.802 kb on + strand, within MMJJ_RS08230
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,512,850 - MMJJ_RS08215 0.22 -0.3 1,512,860 + MMJJ_RS08215 0.23 +0.7 1,512,870 + MMJJ_RS08215 0.24 -0.2 1,512,991 - MMJJ_RS08215 0.35 -0.1 1,512,991 - MMJJ_RS08215 0.35 -0.7 1,512,992 + MMJJ_RS08215 0.35 -0.8 1,512,992 + MMJJ_RS08215 0.35 +0.6 1,513,001 + MMJJ_RS08215 0.36 -0.2 1,513,017 - MMJJ_RS08215 0.37 -0.3 1,513,018 + MMJJ_RS08215 0.38 -0.1 1,513,058 + MMJJ_RS08215 0.41 +0.3 1,513,098 - MMJJ_RS08215 0.45 -0.2 1,513,098 - MMJJ_RS08215 0.45 -0.2 1,513,098 - MMJJ_RS08215 0.45 +0.5 1,513,233 - MMJJ_RS08215 0.57 +1.3 1,513,274 + MMJJ_RS08215 0.60 +0.3 1,513,298 - MMJJ_RS08215 0.63 -0.2 1,513,298 - MMJJ_RS08215 0.63 +0.1 1,513,299 + MMJJ_RS08215 0.63 +0.0 1,513,344 + MMJJ_RS08215 0.67 -0.2 1,513,386 - MMJJ_RS08215 0.71 +0.3 1,513,434 + MMJJ_RS08215 0.75 -0.4 1,513,437 - MMJJ_RS08215 0.75 +0.3 1,513,437 - MMJJ_RS08215 0.75 -0.7 1,513,460 - MMJJ_RS08215 0.77 +0.8 1,513,481 - MMJJ_RS08215 0.79 +0.3 1,513,495 - MMJJ_RS08215 0.80 +0.4 1,513,509 - MMJJ_RS08215 0.82 +0.3 1,513,509 - MMJJ_RS08215 0.82 +0.8 1,513,509 - MMJJ_RS08215 0.82 +0.3 1,513,602 - MMJJ_RS08215 0.90 -0.5 1,513,605 - -0.2 1,513,630 - -0.0 1,513,631 + -0.3 1,513,633 - +0.3 1,513,633 - -0.0 1,513,635 - -0.4 1,513,635 - +0.1 1,513,658 - -1.0 1,513,658 - -0.2 1,513,665 - +0.0 1,513,771 - +0.0 1,513,774 - -0.5 1,513,775 + +0.2 1,513,787 + -0.7 1,513,803 - +2.3 1,513,807 - -0.6 1,513,807 - +0.3 1,513,808 + +0.5 1,513,808 + -0.4 1,513,808 + +0.1 1,513,809 - +2.6 1,513,813 - -0.2 1,513,814 + +0.5 1,513,815 - -0.2 1,513,820 + -0.4 1,513,820 + +1.0 1,513,822 + +0.1 1,513,826 - +0.0 1,513,826 - +0.2 1,513,826 - -0.8 1,513,827 + -1.1 1,513,827 + -0.3 1,513,827 + -0.9 1,513,831 - -0.4 1,513,837 + +0.4 1,513,838 - -0.7 1,513,839 + -0.3 1,513,868 - -0.2 1,513,869 + +1.3 1,513,891 + +0.8 1,513,991 - MMJJ_RS08220 0.16 +1.3 1,514,681 - -1.3 1,514,708 + MMJJ_RS08220 0.90 +0.6 1,514,725 - +0.5 1,514,730 + +0.1 1,514,911 + +0.4 1,514,911 + +0.5 1,514,915 - +0.9 1,514,915 - +1.6 1,514,916 + +0.9 1,514,916 + +0.6 1,514,918 + +0.2 1,514,918 + -0.3 1,514,933 - -0.0 1,514,933 - -1.8 1,514,933 - -0.1 1,514,938 - +1.7 1,514,939 + -1.1 1,514,939 + +0.1 1,514,940 - -1.0 1,514,940 - +0.7 1,514,940 - +0.1 1,514,940 - +0.7 1,514,940 - -0.2 1,514,940 - -0.6 1,514,941 + -0.6 1,514,941 + +0.1 1,514,941 + +0.3 1,514,941 + +0.0 1,514,941 + -0.7 1,514,941 + -1.3 1,514,941 + +1.7 1,514,941 + +0.2 1,514,941 + -0.8 1,514,942 - +0.0 1,514,942 - +2.3 1,514,942 - -0.3 1,514,942 - -0.8 1,514,942 - -0.0 1,514,942 - +0.0 1,514,942 - +2.3 1,514,942 - -0.7 1,514,942 - +0.7 1,514,942 - -0.1 1,514,942 - -1.0 1,514,942 - -0.7 1,514,942 - -0.1 1,514,943 + +0.6 1,514,943 + -0.7 1,514,943 + +0.2 1,514,943 + -0.3 1,514,943 + -0.5 1,514,943 + -0.7 1,514,943 + +0.7 1,514,943 + +0.2 1,514,944 - -0.6 1,514,944 - -0.6 1,514,944 - -0.3 1,514,944 - -0.1 1,514,944 - -0.3 1,514,944 - +0.7 1,514,944 - +0.2 1,514,945 + +0.7 1,514,945 + +0.3 1,514,945 + -0.3 1,514,947 - -0.3 1,514,948 + -1.1 1,514,948 + -2.2 1,514,948 + +1.1 1,514,982 - +0.2 1,514,982 - -0.1 1,515,014 - +0.0 1,515,015 + -1.1 1,515,020 + -1.1 1,515,020 + -0.6 1,515,030 - -0.1 1,515,066 + MMJJ_RS08225 0.15 -0.3 1,515,120 - MMJJ_RS08225 0.27 +2.3 1,515,121 + MMJJ_RS08225 0.27 +2.1 1,515,121 + MMJJ_RS08225 0.27 -0.4 1,515,121 + MMJJ_RS08225 0.27 +0.6 1,515,131 + MMJJ_RS08225 0.30 +1.9 1,515,131 + MMJJ_RS08225 0.30 +0.3 1,515,138 + MMJJ_RS08225 0.31 -0.0 1,515,355 - MMJJ_RS08225 0.79 +0.8 1,515,362 - MMJJ_RS08225 0.81 -0.3 1,515,435 + +1.7 1,515,436 - -0.3 1,515,437 + +0.1 1,515,462 + +0.9 1,515,470 - -1.5 1,515,470 - +0.2 1,515,489 - -0.0 1,515,498 + +0.2 1,515,498 + -0.1 1,515,524 + +2.3 1,515,550 + +1.9 1,515,699 + MMJJ_RS08230 0.25 +0.6 1,515,700 - MMJJ_RS08230 0.25 +0.8 1,515,715 - MMJJ_RS08230 0.27 +0.4 1,515,716 + MMJJ_RS08230 0.28 -0.1 1,515,802 + MMJJ_RS08230 0.39 +0.3
Or see this region's nucleotide sequence