Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08110 and MMJJ_RS08115 are separated by 18 nucleotides MMJJ_RS08115 and MMJJ_RS08120 are separated by 62 nucleotides MMJJ_RS08120 and MMJJ_RS08125 are separated by 135 nucleotides
MMJJ_RS08110: MMJJ_RS08110 - thiolase domain-containing protein, at 1,487,712 to 1,488,890
_RS08110
MMJJ_RS08115: MMJJ_RS08115 - hydroxymethylglutaryl-CoA synthase, at 1,488,909 to 1,489,958
_RS08115
MMJJ_RS08120: MMJJ_RS08120 - helix-turn-helix domain-containing protein, at 1,490,021 to 1,490,710
_RS08120
MMJJ_RS08125: MMJJ_RS08125 - DNA double-strand break repair nuclease NurA, at 1,490,846 to 1,491,910
_RS08125
Position (kb)
1488
1489
1490 Strain fitness (log2 ratio)
-1
0
1 at 1488.125 kb on - strand at 1488.126 kb on + strand, within MMJJ_RS08110 at 1489.156 kb on + strand at 1489.533 kb on + strand, within MMJJ_RS08115 at 1489.722 kb on - strand, within MMJJ_RS08115 at 1489.939 kb on + strand at 1489.948 kb on + strand at 1490.510 kb on - strand at 1490.689 kb on - strand at 1490.813 kb on - strand at 1490.842 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,488,125 - -1.2 1,488,126 + MMJJ_RS08110 0.35 -1.2 1,489,156 + -0.8 1,489,533 + MMJJ_RS08115 0.59 +0.8 1,489,722 - MMJJ_RS08115 0.77 -1.2 1,489,939 + -0.2 1,489,948 + -0.8 1,490,510 - +0.8 1,490,689 - +1.3 1,490,813 - +1.1 1,490,842 - +0.3
Or see this region's nucleotide sequence