Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07895 and MMJJ_RS07900 are separated by 40 nucleotides MMJJ_RS07900 and MMJJ_RS07905 are separated by 62 nucleotides
MMJJ_RS07895: MMJJ_RS07895 - phenylalanine--tRNA ligase subunit beta, at 1,448,314 to 1,449,978
_RS07895
MMJJ_RS07900: MMJJ_RS07900 - phosphoribosylformylglycinamidine cyclo-ligase, at 1,450,019 to 1,451,068
_RS07900
MMJJ_RS07905: MMJJ_RS07905 - DUF89 domain-containing protein, at 1,451,131 to 1,452,030
_RS07905
Position (kb)
1450
1451
1452 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1449.506 kb on - strand, within MMJJ_RS07895 at 1450.003 kb on + strand at 1450.184 kb on - strand, within MMJJ_RS07900 at 1450.305 kb on + strand, within MMJJ_RS07900 at 1451.058 kb on - strand at 1451.139 kb on - strand at 1451.139 kb on - strand at 1451.140 kb on + strand at 1451.143 kb on - strand at 1451.179 kb on - strand at 1451.180 kb on + strand at 1451.223 kb on - strand, within MMJJ_RS07905 at 1451.223 kb on - strand, within MMJJ_RS07905 at 1451.224 kb on + strand, within MMJJ_RS07905 at 1451.229 kb on + strand, within MMJJ_RS07905 at 1451.329 kb on - strand, within MMJJ_RS07905 at 1451.398 kb on - strand, within MMJJ_RS07905 at 1451.399 kb on + strand, within MMJJ_RS07905 at 1451.407 kb on - strand, within MMJJ_RS07905 at 1451.558 kb on - strand, within MMJJ_RS07905 at 1451.563 kb on - strand, within MMJJ_RS07905 at 1451.581 kb on + strand, within MMJJ_RS07905 at 1451.617 kb on - strand, within MMJJ_RS07905 at 1451.642 kb on + strand, within MMJJ_RS07905 at 1451.647 kb on + strand, within MMJJ_RS07905 at 1451.647 kb on + strand, within MMJJ_RS07905 at 1451.671 kb on + strand, within MMJJ_RS07905 at 1451.671 kb on + strand, within MMJJ_RS07905 at 1451.781 kb on - strand, within MMJJ_RS07905 at 1451.782 kb on + strand, within MMJJ_RS07905 at 1451.830 kb on + strand, within MMJJ_RS07905 at 1451.849 kb on + strand, within MMJJ_RS07905 at 1451.849 kb on + strand, within MMJJ_RS07905 at 1451.852 kb on - strand, within MMJJ_RS07905 at 1451.853 kb on + strand, within MMJJ_RS07905 at 1451.875 kb on + strand, within MMJJ_RS07905 at 1451.875 kb on + strand, within MMJJ_RS07905 at 1451.900 kb on - strand, within MMJJ_RS07905 at 1451.933 kb on + strand, within MMJJ_RS07905 at 1451.934 kb on - strand, within MMJJ_RS07905 at 1451.935 kb on + strand, within MMJJ_RS07905 at 1451.956 kb on + strand at 1451.966 kb on + strand at 1451.983 kb on - strand at 1451.984 kb on + strand at 1451.984 kb on + strand at 1451.984 kb on + strand at 1451.984 kb on + strand at 1452.022 kb on + strand at 1452.049 kb on - strand at 1452.049 kb on - strand at 1452.049 kb on - strand at 1452.049 kb on - strand at 1452.049 kb on - strand at 1452.050 kb on + strand at 1452.050 kb on + strand at 1452.050 kb on + strand at 1452.053 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,449,506 - MMJJ_RS07895 0.72 +1.0 1,450,003 + +0.5 1,450,184 - MMJJ_RS07900 0.16 +0.6 1,450,305 + MMJJ_RS07900 0.27 -0.5 1,451,058 - -1.2 1,451,139 - +0.1 1,451,139 - -1.0 1,451,140 + +0.8 1,451,143 - +1.1 1,451,179 - +0.1 1,451,180 + +0.9 1,451,223 - MMJJ_RS07905 0.10 +0.3 1,451,223 - MMJJ_RS07905 0.10 +0.1 1,451,224 + MMJJ_RS07905 0.10 -0.5 1,451,229 + MMJJ_RS07905 0.11 -0.2 1,451,329 - MMJJ_RS07905 0.22 -0.1 1,451,398 - MMJJ_RS07905 0.30 +0.3 1,451,399 + MMJJ_RS07905 0.30 -0.1 1,451,407 - MMJJ_RS07905 0.31 +0.3 1,451,558 - MMJJ_RS07905 0.47 +1.1 1,451,563 - MMJJ_RS07905 0.48 +0.1 1,451,581 + MMJJ_RS07905 0.50 +0.0 1,451,617 - MMJJ_RS07905 0.54 -0.3 1,451,642 + MMJJ_RS07905 0.57 +0.2 1,451,647 + MMJJ_RS07905 0.57 -0.3 1,451,647 + MMJJ_RS07905 0.57 -0.2 1,451,671 + MMJJ_RS07905 0.60 -0.2 1,451,671 + MMJJ_RS07905 0.60 -0.1 1,451,781 - MMJJ_RS07905 0.72 -2.8 1,451,782 + MMJJ_RS07905 0.72 +0.1 1,451,830 + MMJJ_RS07905 0.78 -0.1 1,451,849 + MMJJ_RS07905 0.80 -0.2 1,451,849 + MMJJ_RS07905 0.80 -0.2 1,451,852 - MMJJ_RS07905 0.80 +0.2 1,451,853 + MMJJ_RS07905 0.80 -0.7 1,451,875 + MMJJ_RS07905 0.83 -0.0 1,451,875 + MMJJ_RS07905 0.83 +0.7 1,451,900 - MMJJ_RS07905 0.85 +0.5 1,451,933 + MMJJ_RS07905 0.89 +0.4 1,451,934 - MMJJ_RS07905 0.89 -0.0 1,451,935 + MMJJ_RS07905 0.89 -0.5 1,451,956 + -0.2 1,451,966 + +0.0 1,451,983 - +0.3 1,451,984 + -0.2 1,451,984 + +1.8 1,451,984 + -0.2 1,451,984 + +0.1 1,452,022 + +0.5 1,452,049 - +0.1 1,452,049 - +0.8 1,452,049 - +1.0 1,452,049 - -0.1 1,452,049 - +2.0 1,452,050 + +0.6 1,452,050 + +0.3 1,452,050 + -0.2 1,452,053 + +0.8
Or see this region's nucleotide sequence