Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07310 and MMJJ_RS07315 are separated by 16 nucleotides MMJJ_RS07315 and MMJJ_RS07320 are separated by 197 nucleotides MMJJ_RS07320 and MMJJ_RS07325 are separated by 64 nucleotides
MMJJ_RS07310: MMJJ_RS07310 - NusA-like transcription termination signal-binding factor, at 1,340,354 to 1,340,875
_RS07310
MMJJ_RS07315: MMJJ_RS07315 - 50S ribosomal protein L30e, at 1,340,892 to 1,341,212
_RS07315
MMJJ_RS07320: MMJJ_RS07320 - DNA-directed RNA polymerase subunit A'', at 1,341,410 to 1,342,570
_RS07320
MMJJ_RS07325: MMJJ_RS07325 - 'DNA-directed RNA polymerase subunit A'' pseudo=_no_value transl_table=11, at 1,342,635 to 1,345,283
_RS07325
Position (kb)
1341
1342
1343 Strain fitness (log2 ratio)
-1
0
1
2 at 1341.197 kb on - strand at 1341.327 kb on - strand at 1341.328 kb on + strand at 1341.331 kb on - strand at 1341.331 kb on - strand at 1341.332 kb on + strand at 1341.332 kb on + strand at 1341.333 kb on - strand at 1341.333 kb on - strand at 1341.333 kb on - strand at 1341.338 kb on + strand at 1341.357 kb on - strand at 1341.357 kb on - strand at 1341.357 kb on - strand at 1341.358 kb on + strand at 1341.366 kb on - strand at 1341.381 kb on + strand at 1341.433 kb on + strand at 1341.449 kb on - strand at 1341.449 kb on - strand at 1341.450 kb on + strand at 1341.450 kb on + strand at 1342.106 kb on + strand, within MMJJ_RS07320 at 1342.581 kb on - strand at 1342.612 kb on - strand at 1342.699 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,341,197 - +0.4 1,341,327 - -0.2 1,341,328 + +0.1 1,341,331 - +0.2 1,341,331 - -0.2 1,341,332 + +0.1 1,341,332 + +0.1 1,341,333 - +0.3 1,341,333 - -0.8 1,341,333 - -0.4 1,341,338 + +0.2 1,341,357 - -0.4 1,341,357 - -1.1 1,341,357 - +0.2 1,341,358 + +0.2 1,341,366 - -0.1 1,341,381 + +0.2 1,341,433 + -0.1 1,341,449 - +1.6 1,341,449 - +0.5 1,341,450 + +1.2 1,341,450 + +0.2 1,342,106 + MMJJ_RS07320 0.60 -0.2 1,342,581 - +1.8 1,342,612 - +1.9 1,342,699 + +0.4
Or see this region's nucleotide sequence