Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06325 and MMJJ_RS06330 are separated by 36 nucleotides MMJJ_RS06330 and MMJJ_RS06335 are separated by 26 nucleotides MMJJ_RS06335 and MMJJ_RS06340 are separated by 267 nucleotides
MMJJ_RS06325: MMJJ_RS06325 - tetrahydromethanopterin S-methyltransferase subunit C, at 1,171,194 to 1,171,982
_RS06325
MMJJ_RS06330: MMJJ_RS06330 - tetrahydromethanopterin S-methyltransferase subunit D, at 1,172,019 to 1,172,726
_RS06330
MMJJ_RS06335: MMJJ_RS06335 - tetrahydromethanopterin S-methyltransferase subunit E, at 1,172,753 to 1,173,652
_RS06335
MMJJ_RS06340: MMJJ_RS06340 - coenzyme-B sulfoethylthiotransferase subunit alpha, at 1,173,920 to 1,175,581
_RS06340
Position (kb)
1172
1173
1174 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1171.779 kb on + strand, within MMJJ_RS06325 at 1171.863 kb on + strand, within MMJJ_RS06325 at 1171.987 kb on + strand at 1171.987 kb on + strand at 1172.240 kb on + strand, within MMJJ_RS06330 at 1172.479 kb on + strand, within MMJJ_RS06330 at 1172.602 kb on + strand, within MMJJ_RS06330 at 1172.939 kb on - strand, within MMJJ_RS06335 at 1172.948 kb on + strand, within MMJJ_RS06335 at 1173.451 kb on + strand at 1173.716 kb on - strand at 1173.722 kb on - strand at 1173.762 kb on - strand at 1173.763 kb on + strand at 1173.763 kb on + strand at 1173.845 kb on + strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.875 kb on + strand at 1173.875 kb on + strand at 1173.875 kb on + strand at 1173.875 kb on + strand at 1173.875 kb on + strand at 1173.878 kb on - strand at 1173.879 kb on + strand at 1173.880 kb on - strand at 1173.933 kb on + strand at 1174.331 kb on + strand, within MMJJ_RS06340 at 1174.334 kb on - strand at 1174.335 kb on + strand, within MMJJ_RS06340 at 1174.420 kb on + strand, within MMJJ_RS06340 at 1174.527 kb on - strand, within MMJJ_RS06340
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,171,779 + MMJJ_RS06325 0.74 -1.2 1,171,863 + MMJJ_RS06325 0.85 -2.5 1,171,987 + +0.4 1,171,987 + +0.5 1,172,240 + MMJJ_RS06330 0.31 -0.2 1,172,479 + MMJJ_RS06330 0.65 -0.4 1,172,602 + MMJJ_RS06330 0.82 +0.1 1,172,939 - MMJJ_RS06335 0.21 +1.8 1,172,948 + MMJJ_RS06335 0.22 -0.4 1,173,451 + -0.2 1,173,716 - -2.2 1,173,722 - +0.2 1,173,762 - -2.2 1,173,763 + -0.8 1,173,763 + +0.8 1,173,845 + +0.6 1,173,874 - +0.5 1,173,874 - +0.2 1,173,874 - +0.3 1,173,874 - +0.7 1,173,874 - -0.0 1,173,874 - +0.0 1,173,874 - -0.8 1,173,874 - +1.2 1,173,875 + -0.1 1,173,875 + +0.5 1,173,875 + -0.5 1,173,875 + +0.8 1,173,875 + +0.1 1,173,878 - -0.9 1,173,879 + -0.8 1,173,880 - +0.8 1,173,933 + +0.7 1,174,331 + MMJJ_RS06340 0.25 -0.1 1,174,334 - +0.8 1,174,335 + MMJJ_RS06340 0.25 +0.6 1,174,420 + MMJJ_RS06340 0.30 +1.8 1,174,527 - MMJJ_RS06340 0.37 +0.4
Or see this region's nucleotide sequence