Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03465 and MMJJ_RS03470 are separated by 17 nucleotides MMJJ_RS03470 and MMJJ_RS03475 are separated by 21 nucleotides MMJJ_RS03475 and MMJJ_RS03480 are separated by 8 nucleotides
MMJJ_RS03465: MMJJ_RS03465 - restriction endonuclease, at 649,522 to 650,451
_RS03465
MMJJ_RS03470: MMJJ_RS03470 - N-6 DNA methylase, at 650,469 to 651,977
_RS03470
MMJJ_RS03475: MMJJ_RS03475 - tyrosine-type recombinase/integrase, at 651,999 to 652,886
_RS03475
MMJJ_RS03480: MMJJ_RS03480 - hypothetical protein, at 652,895 to 653,443
_RS03480
Position (kb) 650
651
652 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 649.470 kb on + strand at 649.482 kb on + strand at 649.488 kb on + strand at 649.488 kb on + strand at 649.508 kb on + strand at 649.519 kb on + strand at 649.551 kb on - strand at 649.552 kb on + strand at 649.552 kb on + strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.554 kb on + strand at 649.554 kb on + strand at 649.554 kb on + strand at 649.558 kb on - strand at 649.558 kb on - strand at 649.559 kb on + strand at 649.559 kb on + strand at 649.559 kb on + strand at 649.559 kb on + strand at 649.559 kb on + strand at 649.609 kb on + strand at 649.609 kb on + strand at 649.619 kb on - strand, within MMJJ_RS03465 at 649.620 kb on + strand, within MMJJ_RS03465 at 649.620 kb on + strand, within MMJJ_RS03465 at 649.632 kb on - strand, within MMJJ_RS03465 at 649.633 kb on + strand, within MMJJ_RS03465 at 649.633 kb on + strand, within MMJJ_RS03465 at 649.644 kb on - strand, within MMJJ_RS03465 at 649.658 kb on - strand, within MMJJ_RS03465 at 649.658 kb on - strand, within MMJJ_RS03465 at 649.659 kb on + strand, within MMJJ_RS03465 at 649.659 kb on + strand, within MMJJ_RS03465 at 649.659 kb on + strand, within MMJJ_RS03465 at 649.660 kb on - strand, within MMJJ_RS03465 at 649.660 kb on - strand, within MMJJ_RS03465 at 649.661 kb on + strand, within MMJJ_RS03465 at 649.661 kb on + strand, within MMJJ_RS03465 at 649.674 kb on - strand, within MMJJ_RS03465 at 649.674 kb on - strand, within MMJJ_RS03465 at 649.674 kb on - strand, within MMJJ_RS03465 at 649.674 kb on - strand, within MMJJ_RS03465 at 649.675 kb on + strand, within MMJJ_RS03465 at 649.675 kb on + strand, within MMJJ_RS03465 at 649.738 kb on + strand, within MMJJ_RS03465 at 649.751 kb on + strand, within MMJJ_RS03465 at 649.800 kb on - strand, within MMJJ_RS03465 at 649.809 kb on - strand, within MMJJ_RS03465 at 649.809 kb on - strand, within MMJJ_RS03465 at 649.852 kb on + strand, within MMJJ_RS03465 at 649.854 kb on + strand, within MMJJ_RS03465 at 649.883 kb on - strand, within MMJJ_RS03465 at 649.884 kb on + strand, within MMJJ_RS03465 at 649.911 kb on - strand, within MMJJ_RS03465 at 649.943 kb on + strand at 649.951 kb on + strand at 649.951 kb on + strand, within MMJJ_RS03465 at 649.992 kb on - strand, within MMJJ_RS03465 at 650.005 kb on + strand, within MMJJ_RS03465 at 650.049 kb on - strand, within MMJJ_RS03465 at 650.050 kb on + strand, within MMJJ_RS03465 at 650.097 kb on + strand, within MMJJ_RS03465 at 650.097 kb on + strand, within MMJJ_RS03465 at 650.146 kb on + strand, within MMJJ_RS03465 at 650.146 kb on + strand, within MMJJ_RS03465 at 650.215 kb on - strand, within MMJJ_RS03465 at 650.216 kb on + strand, within MMJJ_RS03465 at 650.216 kb on + strand, within MMJJ_RS03465 at 650.216 kb on + strand, within MMJJ_RS03465 at 650.216 kb on + strand, within MMJJ_RS03465 at 650.230 kb on - strand, within MMJJ_RS03465 at 650.231 kb on + strand, within MMJJ_RS03465 at 650.255 kb on - strand, within MMJJ_RS03465 at 650.256 kb on + strand, within MMJJ_RS03465 at 650.259 kb on - strand, within MMJJ_RS03465 at 650.259 kb on - strand, within MMJJ_RS03465 at 650.259 kb on - strand, within MMJJ_RS03465 at 650.259 kb on - strand, within MMJJ_RS03465 at 650.260 kb on + strand, within MMJJ_RS03465 at 650.260 kb on + strand, within MMJJ_RS03465 at 650.260 kb on + strand, within MMJJ_RS03465 at 650.260 kb on + strand, within MMJJ_RS03465 at 650.262 kb on + strand, within MMJJ_RS03465 at 650.268 kb on + strand, within MMJJ_RS03465 at 650.281 kb on + strand, within MMJJ_RS03465 at 650.308 kb on - strand, within MMJJ_RS03465 at 650.309 kb on + strand, within MMJJ_RS03465 at 650.370 kb on - strand at 650.370 kb on - strand at 650.383 kb on + strand at 650.424 kb on - strand at 650.425 kb on + strand at 650.428 kb on - strand at 650.475 kb on + strand at 650.478 kb on + strand at 650.478 kb on + strand at 650.478 kb on + strand at 650.478 kb on + strand at 650.483 kb on - strand at 650.484 kb on + strand at 650.484 kb on + strand at 650.487 kb on + strand at 650.657 kb on - strand, within MMJJ_RS03470 at 650.719 kb on - strand, within MMJJ_RS03470 at 651.147 kb on + strand, within MMJJ_RS03470 at 651.197 kb on - strand, within MMJJ_RS03470 at 652.007 kb on - strand at 652.007 kb on - strand at 652.016 kb on - strand at 652.027 kb on - strand at 652.064 kb on + strand at 652.076 kb on - strand at 652.077 kb on + strand at 652.155 kb on + strand, within MMJJ_RS03475 at 652.175 kb on - strand, within MMJJ_RS03475 at 652.212 kb on - strand, within MMJJ_RS03475 at 652.216 kb on - strand, within MMJJ_RS03475 at 652.233 kb on - strand, within MMJJ_RS03475 at 652.318 kb on - strand, within MMJJ_RS03475 at 652.318 kb on - strand, within MMJJ_RS03475 at 652.335 kb on - strand, within MMJJ_RS03475 at 652.356 kb on - strand, within MMJJ_RS03475 at 652.356 kb on - strand, within MMJJ_RS03475 at 652.356 kb on - strand, within MMJJ_RS03475 at 652.357 kb on + strand, within MMJJ_RS03475 at 652.360 kb on - strand, within MMJJ_RS03475 at 652.360 kb on - strand, within MMJJ_RS03475 at 652.360 kb on - strand, within MMJJ_RS03475 at 652.360 kb on - strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.364 kb on - strand, within MMJJ_RS03475 at 652.417 kb on + strand, within MMJJ_RS03475 at 652.425 kb on - strand, within MMJJ_RS03475 at 652.425 kb on - strand, within MMJJ_RS03475 at 652.426 kb on + strand, within MMJJ_RS03475 at 652.431 kb on - strand, within MMJJ_RS03475 at 652.476 kb on - strand, within MMJJ_RS03475 at 652.483 kb on - strand, within MMJJ_RS03475 at 652.484 kb on + strand, within MMJJ_RS03475 at 652.489 kb on - strand, within MMJJ_RS03475 at 652.492 kb on - strand, within MMJJ_RS03475 at 652.492 kb on - strand, within MMJJ_RS03475 at 652.498 kb on - strand, within MMJJ_RS03475 at 652.507 kb on + strand, within MMJJ_RS03475 at 652.637 kb on + strand, within MMJJ_RS03475 at 652.709 kb on - strand, within MMJJ_RS03475 at 652.709 kb on - strand, within MMJJ_RS03475 at 652.710 kb on + strand, within MMJJ_RS03475 at 652.711 kb on - strand, within MMJJ_RS03475 at 652.720 kb on - strand, within MMJJ_RS03475 at 652.720 kb on - strand, within MMJJ_RS03475 at 652.720 kb on - strand, within MMJJ_RS03475 at 652.721 kb on + strand, within MMJJ_RS03475 at 652.721 kb on + strand, within MMJJ_RS03475 at 652.728 kb on - strand, within MMJJ_RS03475 at 652.740 kb on - strand, within MMJJ_RS03475 at 652.741 kb on + strand, within MMJJ_RS03475 at 652.749 kb on + strand, within MMJJ_RS03475 at 652.805 kb on - strand at 652.806 kb on + strand at 652.810 kb on - strand at 652.816 kb on + strand at 652.816 kb on + strand at 652.889 kb on - strand at 652.910 kb on - strand at 652.910 kb on - strand at 652.910 kb on - strand at 652.940 kb on - strand at 652.940 kb on - strand at 652.940 kb on - strand at 652.941 kb on + strand at 652.941 kb on + strand at 652.941 kb on + strand at 652.948 kb on - strand at 652.954 kb on - strand, within MMJJ_RS03480 at 652.961 kb on - strand, within MMJJ_RS03480 at 652.961 kb on - strand, within MMJJ_RS03480 at 652.962 kb on + strand, within MMJJ_RS03480
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 649,470 + +2.8 649,482 + +0.4 649,488 + +0.4 649,488 + +0.7 649,508 + -0.1 649,519 + -1.0 649,551 - +0.0 649,552 + -0.2 649,552 + +0.0 649,553 - -0.8 649,553 - +0.1 649,553 - +0.1 649,553 - +0.3 649,553 - +0.4 649,553 - -0.2 649,553 - +2.5 649,554 + +1.8 649,554 + -0.8 649,554 + +0.4 649,558 - +0.5 649,558 - -0.8 649,559 + -0.0 649,559 + +0.9 649,559 + -0.6 649,559 + -0.5 649,559 + +0.2 649,609 + +0.2 649,609 + -0.2 649,619 - MMJJ_RS03465 0.10 +0.3 649,620 + MMJJ_RS03465 0.11 -0.3 649,620 + MMJJ_RS03465 0.11 -0.8 649,632 - MMJJ_RS03465 0.12 -0.3 649,633 + MMJJ_RS03465 0.12 -0.0 649,633 + MMJJ_RS03465 0.12 -0.2 649,644 - MMJJ_RS03465 0.13 -0.9 649,658 - MMJJ_RS03465 0.15 -0.1 649,658 - MMJJ_RS03465 0.15 -1.1 649,659 + MMJJ_RS03465 0.15 +0.7 649,659 + MMJJ_RS03465 0.15 +1.0 649,659 + MMJJ_RS03465 0.15 +0.1 649,660 - MMJJ_RS03465 0.15 +0.9 649,660 - MMJJ_RS03465 0.15 -0.6 649,661 + MMJJ_RS03465 0.15 -0.2 649,661 + MMJJ_RS03465 0.15 -0.6 649,674 - MMJJ_RS03465 0.16 +0.2 649,674 - MMJJ_RS03465 0.16 +0.6 649,674 - MMJJ_RS03465 0.16 -1.3 649,674 - MMJJ_RS03465 0.16 +0.4 649,675 + MMJJ_RS03465 0.16 -0.3 649,675 + MMJJ_RS03465 0.16 +0.8 649,738 + MMJJ_RS03465 0.23 +1.0 649,751 + MMJJ_RS03465 0.25 +0.3 649,800 - MMJJ_RS03465 0.30 +0.2 649,809 - MMJJ_RS03465 0.31 -0.2 649,809 - MMJJ_RS03465 0.31 +0.7 649,852 + MMJJ_RS03465 0.35 +0.6 649,854 + MMJJ_RS03465 0.36 -1.5 649,883 - MMJJ_RS03465 0.39 -0.4 649,884 + MMJJ_RS03465 0.39 +2.4 649,911 - MMJJ_RS03465 0.42 +0.3 649,943 + -0.2 649,951 + +1.8 649,951 + MMJJ_RS03465 0.46 -0.1 649,992 - MMJJ_RS03465 0.51 +0.2 650,005 + MMJJ_RS03465 0.52 +0.6 650,049 - MMJJ_RS03465 0.57 -0.5 650,050 + MMJJ_RS03465 0.57 -0.7 650,097 + MMJJ_RS03465 0.62 +1.3 650,097 + MMJJ_RS03465 0.62 +0.1 650,146 + MMJJ_RS03465 0.67 -0.2 650,146 + MMJJ_RS03465 0.67 +0.4 650,215 - MMJJ_RS03465 0.75 +0.4 650,216 + MMJJ_RS03465 0.75 +0.3 650,216 + MMJJ_RS03465 0.75 +0.0 650,216 + MMJJ_RS03465 0.75 +0.1 650,216 + MMJJ_RS03465 0.75 -0.2 650,230 - MMJJ_RS03465 0.76 -0.1 650,231 + MMJJ_RS03465 0.76 +0.2 650,255 - MMJJ_RS03465 0.79 -0.2 650,256 + MMJJ_RS03465 0.79 +0.1 650,259 - MMJJ_RS03465 0.79 -0.7 650,259 - MMJJ_RS03465 0.79 -1.8 650,259 - MMJJ_RS03465 0.79 +0.4 650,259 - MMJJ_RS03465 0.79 -0.6 650,260 + MMJJ_RS03465 0.79 -0.3 650,260 + MMJJ_RS03465 0.79 -0.5 650,260 + MMJJ_RS03465 0.79 -1.2 650,260 + MMJJ_RS03465 0.79 +1.6 650,262 + MMJJ_RS03465 0.80 +0.8 650,268 + MMJJ_RS03465 0.80 +0.5 650,281 + MMJJ_RS03465 0.82 +0.6 650,308 - MMJJ_RS03465 0.85 -0.1 650,309 + MMJJ_RS03465 0.85 -0.6 650,370 - +0.0 650,370 - -1.3 650,383 + -0.6 650,424 - -0.0 650,425 + +0.2 650,428 - +0.1 650,475 + +0.5 650,478 + -0.2 650,478 + +0.8 650,478 + +0.1 650,478 + +0.2 650,483 - -0.5 650,484 + -0.1 650,484 + +0.5 650,487 + -0.2 650,657 - MMJJ_RS03470 0.12 -3.2 650,719 - MMJJ_RS03470 0.17 -0.2 651,147 + MMJJ_RS03470 0.45 +2.2 651,197 - MMJJ_RS03470 0.48 +0.4 652,007 - -0.4 652,007 - -0.2 652,016 - +0.3 652,027 - +0.2 652,064 + +1.1 652,076 - -0.7 652,077 + +0.1 652,155 + MMJJ_RS03475 0.18 +0.1 652,175 - MMJJ_RS03475 0.20 +2.4 652,212 - MMJJ_RS03475 0.24 -0.8 652,216 - MMJJ_RS03475 0.24 -0.7 652,233 - MMJJ_RS03475 0.26 +0.4 652,318 - MMJJ_RS03475 0.36 -0.2 652,318 - MMJJ_RS03475 0.36 -0.1 652,335 - MMJJ_RS03475 0.38 -0.9 652,356 - MMJJ_RS03475 0.40 -0.1 652,356 - MMJJ_RS03475 0.40 +0.1 652,356 - MMJJ_RS03475 0.40 -0.0 652,357 + MMJJ_RS03475 0.40 +0.0 652,360 - MMJJ_RS03475 0.41 -0.4 652,360 - MMJJ_RS03475 0.41 +0.3 652,360 - MMJJ_RS03475 0.41 -0.8 652,360 - MMJJ_RS03475 0.41 +0.7 652,361 + MMJJ_RS03475 0.41 +1.2 652,361 + MMJJ_RS03475 0.41 +0.3 652,361 + MMJJ_RS03475 0.41 -0.3 652,361 + MMJJ_RS03475 0.41 -0.3 652,361 + MMJJ_RS03475 0.41 +1.2 652,361 + MMJJ_RS03475 0.41 +0.2 652,361 + MMJJ_RS03475 0.41 +0.5 652,364 - MMJJ_RS03475 0.41 +0.6 652,417 + MMJJ_RS03475 0.47 -0.4 652,425 - MMJJ_RS03475 0.48 +0.1 652,425 - MMJJ_RS03475 0.48 -0.1 652,426 + MMJJ_RS03475 0.48 -0.2 652,431 - MMJJ_RS03475 0.49 -0.6 652,476 - MMJJ_RS03475 0.54 +0.0 652,483 - MMJJ_RS03475 0.55 -0.3 652,484 + MMJJ_RS03475 0.55 -0.4 652,489 - MMJJ_RS03475 0.55 -0.1 652,492 - MMJJ_RS03475 0.56 +1.2 652,492 - MMJJ_RS03475 0.56 -0.5 652,498 - MMJJ_RS03475 0.56 +0.9 652,507 + MMJJ_RS03475 0.57 -0.5 652,637 + MMJJ_RS03475 0.72 -0.2 652,709 - MMJJ_RS03475 0.80 -0.5 652,709 - MMJJ_RS03475 0.80 +0.1 652,710 + MMJJ_RS03475 0.80 -0.1 652,711 - MMJJ_RS03475 0.80 -0.8 652,720 - MMJJ_RS03475 0.81 -0.2 652,720 - MMJJ_RS03475 0.81 +0.8 652,720 - MMJJ_RS03475 0.81 -0.2 652,721 + MMJJ_RS03475 0.81 +0.2 652,721 + MMJJ_RS03475 0.81 -0.0 652,728 - MMJJ_RS03475 0.82 -0.3 652,740 - MMJJ_RS03475 0.83 +0.6 652,741 + MMJJ_RS03475 0.84 +0.6 652,749 + MMJJ_RS03475 0.84 +0.3 652,805 - -0.2 652,806 + -0.4 652,810 - -0.5 652,816 + -0.0 652,816 + -0.3 652,889 - +0.2 652,910 - -0.5 652,910 - +0.0 652,910 - -0.1 652,940 - -0.3 652,940 - -0.1 652,940 - -0.2 652,941 + -0.1 652,941 + +0.5 652,941 + +0.1 652,948 - +0.4 652,954 - MMJJ_RS03480 0.11 +0.3 652,961 - MMJJ_RS03480 0.12 -2.0 652,961 - MMJJ_RS03480 0.12 -0.7 652,962 + MMJJ_RS03480 0.12 +0.4
Or see this region's nucleotide sequence