Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35965 and RR42_RS35970 are separated by 101 nucleotides RR42_RS35970 and RR42_RS35975 are separated by 44 nucleotides RR42_RS35975 and RR42_RS35980 are separated by 12 nucleotides RR42_RS35980 and RR42_RS35985 overlap by 8 nucleotides
RR42_RS35965: RR42_RS35965 - hypothetical protein, at 3,517,800 to 3,518,135
_RS35965
RR42_RS35970: RR42_RS35970 - pilus assembly protein TadD, at 3,518,237 to 3,519,079
_RS35970
RR42_RS35975: RR42_RS35975 - pilus assembly protein TadC, at 3,519,124 to 3,520,140
_RS35975
RR42_RS35980: RR42_RS35980 - membrane protein, at 3,520,153 to 3,521,079
_RS35980
RR42_RS35985: RR42_RS35985 - pilus assembly protein CpaF, at 3,521,072 to 3,522,388
_RS35985
Position (kb)
3519
3520
3521 Strain fitness (log2 ratio)
-1
0
1
2 at 3518.707 kb on - strand, within RR42_RS35970 at 3518.746 kb on - strand, within RR42_RS35970 at 3518.868 kb on + strand, within RR42_RS35970 at 3518.868 kb on + strand, within RR42_RS35970 at 3518.869 kb on - strand, within RR42_RS35970 at 3518.869 kb on - strand, within RR42_RS35970 at 3518.869 kb on - strand, within RR42_RS35970 at 3518.907 kb on + strand, within RR42_RS35970 at 3518.908 kb on - strand, within RR42_RS35970 at 3518.917 kb on + strand, within RR42_RS35970 at 3518.925 kb on + strand, within RR42_RS35970 at 3518.925 kb on + strand, within RR42_RS35970 at 3518.925 kb on + strand, within RR42_RS35970 at 3518.926 kb on - strand, within RR42_RS35970 at 3518.926 kb on - strand, within RR42_RS35970 at 3519.145 kb on - strand at 3519.161 kb on - strand at 3519.248 kb on - strand, within RR42_RS35975 at 3519.354 kb on + strand, within RR42_RS35975 at 3519.767 kb on - strand, within RR42_RS35975 at 3520.129 kb on - strand at 3520.129 kb on - strand at 3520.129 kb on - strand at 3520.155 kb on - strand at 3520.176 kb on - strand at 3520.223 kb on - strand at 3520.262 kb on + strand, within RR42_RS35980 at 3520.263 kb on - strand, within RR42_RS35980 at 3520.263 kb on - strand, within RR42_RS35980 at 3520.488 kb on - strand, within RR42_RS35980 at 3520.488 kb on - strand, within RR42_RS35980 at 3520.646 kb on - strand, within RR42_RS35980 at 3520.722 kb on - strand, within RR42_RS35980 at 3520.796 kb on - strand, within RR42_RS35980 at 3520.931 kb on + strand, within RR42_RS35980 at 3521.073 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,518,707 - RR42_RS35970 0.56 +0.8 3,518,746 - RR42_RS35970 0.60 +0.1 3,518,868 + RR42_RS35970 0.75 +0.9 3,518,868 + RR42_RS35970 0.75 -1.4 3,518,869 - RR42_RS35970 0.75 +1.5 3,518,869 - RR42_RS35970 0.75 +0.2 3,518,869 - RR42_RS35970 0.75 +0.2 3,518,907 + RR42_RS35970 0.79 -0.8 3,518,908 - RR42_RS35970 0.80 -0.3 3,518,917 + RR42_RS35970 0.81 +1.5 3,518,925 + RR42_RS35970 0.82 +1.6 3,518,925 + RR42_RS35970 0.82 +0.3 3,518,925 + RR42_RS35970 0.82 +0.4 3,518,926 - RR42_RS35970 0.82 +1.2 3,518,926 - RR42_RS35970 0.82 +0.0 3,519,145 - +0.9 3,519,161 - +1.4 3,519,248 - RR42_RS35975 0.12 +1.5 3,519,354 + RR42_RS35975 0.23 +0.9 3,519,767 - RR42_RS35975 0.63 +1.1 3,520,129 - +0.9 3,520,129 - +0.4 3,520,129 - +0.7 3,520,155 - +1.3 3,520,176 - +1.8 3,520,223 - +1.1 3,520,262 + RR42_RS35980 0.12 +0.1 3,520,263 - RR42_RS35980 0.12 +0.0 3,520,263 - RR42_RS35980 0.12 +2.0 3,520,488 - RR42_RS35980 0.36 -0.7 3,520,488 - RR42_RS35980 0.36 +1.0 3,520,646 - RR42_RS35980 0.53 +0.9 3,520,722 - RR42_RS35980 0.61 +1.4 3,520,796 - RR42_RS35980 0.69 +0.1 3,520,931 + RR42_RS35980 0.84 +2.2 3,521,073 + +0.3
Or see this region's nucleotide sequence