Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35070 and RR42_RS35075 are separated by 9 nucleotides RR42_RS35075 and RR42_RS35080 are separated by 148 nucleotides
RR42_RS35070: RR42_RS35070 - cobyrinic acid a,c-diamide synthase, at 3,320,465 to 3,321,742
_RS35070
RR42_RS35075: RR42_RS35075 - AraC family transcriptional regulator, at 3,321,752 to 3,322,546
_RS35075
RR42_RS35080: RR42_RS35080 - membrane protein, at 3,322,695 to 3,323,588
_RS35080
Position (kb)
3321
3322
3323 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3320.785 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3321.214 kb on + strand, within RR42_RS35070 at 3321.215 kb on - strand, within RR42_RS35070 at 3321.215 kb on - strand, within RR42_RS35070 at 3321.268 kb on + strand, within RR42_RS35070 at 3321.269 kb on - strand, within RR42_RS35070 at 3321.455 kb on - strand, within RR42_RS35070 at 3321.572 kb on + strand, within RR42_RS35070 at 3321.677 kb on + strand at 3321.689 kb on + strand at 3321.689 kb on + strand at 3321.689 kb on + strand at 3321.690 kb on - strand at 3321.690 kb on - strand at 3321.690 kb on - strand at 3322.092 kb on + strand, within RR42_RS35075 at 3322.276 kb on + strand, within RR42_RS35075 at 3322.278 kb on + strand, within RR42_RS35075 at 3322.279 kb on - strand, within RR42_RS35075 at 3322.279 kb on - strand, within RR42_RS35075 at 3322.279 kb on - strand, within RR42_RS35075 at 3322.351 kb on - strand, within RR42_RS35075 at 3322.351 kb on - strand, within RR42_RS35075 at 3322.379 kb on + strand, within RR42_RS35075 at 3322.413 kb on + strand, within RR42_RS35075 at 3322.413 kb on + strand, within RR42_RS35075 at 3322.414 kb on - strand, within RR42_RS35075 at 3322.414 kb on - strand, within RR42_RS35075 at 3322.432 kb on + strand, within RR42_RS35075 at 3322.432 kb on + strand, within RR42_RS35075 at 3322.432 kb on + strand, within RR42_RS35075 at 3322.433 kb on - strand, within RR42_RS35075 at 3322.433 kb on - strand, within RR42_RS35075 at 3322.433 kb on - strand, within RR42_RS35075 at 3322.485 kb on + strand at 3322.485 kb on + strand at 3322.485 kb on + strand at 3322.486 kb on - strand at 3322.486 kb on - strand at 3322.588 kb on + strand at 3322.589 kb on - strand at 3322.589 kb on - strand at 3322.600 kb on - strand at 3322.600 kb on - strand at 3322.620 kb on - strand at 3322.620 kb on - strand at 3322.620 kb on - strand at 3322.635 kb on + strand at 3322.639 kb on + strand at 3322.639 kb on + strand at 3322.640 kb on - strand at 3322.640 kb on - strand at 3322.665 kb on - strand at 3322.665 kb on - strand at 3322.674 kb on + strand at 3322.674 kb on + strand at 3322.674 kb on + strand at 3322.674 kb on + strand at 3322.675 kb on - strand at 3322.675 kb on - strand at 3322.675 kb on - strand at 3322.675 kb on - strand at 3322.675 kb on - strand at 3322.704 kb on + strand at 3322.704 kb on + strand at 3322.704 kb on + strand at 3322.705 kb on - strand at 3322.705 kb on - strand at 3322.705 kb on - strand at 3322.800 kb on + strand, within RR42_RS35080 at 3322.800 kb on + strand, within RR42_RS35080 at 3322.800 kb on + strand, within RR42_RS35080 at 3322.800 kb on + strand, within RR42_RS35080 at 3322.801 kb on - strand, within RR42_RS35080 at 3322.801 kb on - strand, within RR42_RS35080 at 3322.927 kb on - strand, within RR42_RS35080 at 3322.941 kb on + strand, within RR42_RS35080 at 3322.941 kb on + strand, within RR42_RS35080 at 3322.941 kb on + strand, within RR42_RS35080 at 3322.941 kb on + strand, within RR42_RS35080 at 3322.942 kb on - strand, within RR42_RS35080 at 3322.942 kb on - strand, within RR42_RS35080 at 3322.942 kb on - strand, within RR42_RS35080 at 3322.944 kb on + strand, within RR42_RS35080 at 3322.944 kb on + strand, within RR42_RS35080 at 3322.944 kb on + strand, within RR42_RS35080 at 3322.944 kb on + strand, within RR42_RS35080 at 3322.944 kb on + strand, within RR42_RS35080 at 3322.945 kb on - strand, within RR42_RS35080 at 3322.945 kb on - strand, within RR42_RS35080 at 3322.945 kb on - strand, within RR42_RS35080 at 3322.945 kb on - strand, within RR42_RS35080 at 3322.945 kb on - strand, within RR42_RS35080 at 3322.945 kb on - strand, within RR42_RS35080 at 3322.945 kb on - strand, within RR42_RS35080 at 3322.953 kb on + strand, within RR42_RS35080 at 3323.030 kb on - strand, within RR42_RS35080 at 3323.030 kb on - strand, within RR42_RS35080 at 3323.030 kb on - strand, within RR42_RS35080 at 3323.101 kb on + strand, within RR42_RS35080 at 3323.181 kb on + strand, within RR42_RS35080 at 3323.190 kb on + strand, within RR42_RS35080 at 3323.190 kb on + strand, within RR42_RS35080 at 3323.190 kb on + strand, within RR42_RS35080 at 3323.190 kb on + strand, within RR42_RS35080 at 3323.191 kb on - strand, within RR42_RS35080 at 3323.191 kb on - strand, within RR42_RS35080 at 3323.191 kb on - strand, within RR42_RS35080 at 3323.191 kb on - strand, within RR42_RS35080 at 3323.247 kb on + strand, within RR42_RS35080 at 3323.247 kb on + strand, within RR42_RS35080 at 3323.248 kb on - strand, within RR42_RS35080 at 3323.248 kb on - strand, within RR42_RS35080 at 3323.288 kb on - strand, within RR42_RS35080 at 3323.289 kb on + strand, within RR42_RS35080 at 3323.289 kb on + strand, within RR42_RS35080 at 3323.290 kb on - strand, within RR42_RS35080 at 3323.290 kb on - strand, within RR42_RS35080 at 3323.290 kb on - strand, within RR42_RS35080 at 3323.290 kb on - strand, within RR42_RS35080 at 3323.496 kb on + strand, within RR42_RS35080 at 3323.497 kb on - strand, within RR42_RS35080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,320,785 + RR42_RS35070 0.25 +0.5 3,320,941 + RR42_RS35070 0.37 +0.5 3,320,941 + RR42_RS35070 0.37 +0.0 3,320,941 + RR42_RS35070 0.37 +0.8 3,320,941 + RR42_RS35070 0.37 -1.5 3,320,941 + RR42_RS35070 0.37 +0.6 3,320,942 - RR42_RS35070 0.37 -0.7 3,320,942 - RR42_RS35070 0.37 +0.6 3,320,942 - RR42_RS35070 0.37 +0.5 3,320,942 - RR42_RS35070 0.37 +0.3 3,320,942 - RR42_RS35070 0.37 +0.4 3,320,942 - RR42_RS35070 0.37 -0.9 3,321,214 + RR42_RS35070 0.59 +1.2 3,321,215 - RR42_RS35070 0.59 +0.3 3,321,215 - RR42_RS35070 0.59 -0.1 3,321,268 + RR42_RS35070 0.63 +0.0 3,321,269 - RR42_RS35070 0.63 -1.3 3,321,455 - RR42_RS35070 0.77 -2.3 3,321,572 + RR42_RS35070 0.87 +0.1 3,321,677 + +0.1 3,321,689 + +0.3 3,321,689 + +1.8 3,321,689 + -0.9 3,321,690 - -0.1 3,321,690 - -1.5 3,321,690 - +2.3 3,322,092 + RR42_RS35075 0.43 -0.5 3,322,276 + RR42_RS35075 0.66 -0.6 3,322,278 + RR42_RS35075 0.66 -1.3 3,322,279 - RR42_RS35075 0.66 -1.0 3,322,279 - RR42_RS35075 0.66 +1.3 3,322,279 - RR42_RS35075 0.66 -0.5 3,322,351 - RR42_RS35075 0.75 +0.6 3,322,351 - RR42_RS35075 0.75 -0.3 3,322,379 + RR42_RS35075 0.79 +1.2 3,322,413 + RR42_RS35075 0.83 -0.5 3,322,413 + RR42_RS35075 0.83 -1.4 3,322,414 - RR42_RS35075 0.83 -0.3 3,322,414 - RR42_RS35075 0.83 -0.1 3,322,432 + RR42_RS35075 0.86 -0.5 3,322,432 + RR42_RS35075 0.86 -0.7 3,322,432 + RR42_RS35075 0.86 +0.9 3,322,433 - RR42_RS35075 0.86 -1.0 3,322,433 - RR42_RS35075 0.86 +1.0 3,322,433 - RR42_RS35075 0.86 +0.4 3,322,485 + +1.3 3,322,485 + +0.0 3,322,485 + +0.5 3,322,486 - +0.8 3,322,486 - +0.1 3,322,588 + -0.4 3,322,589 - -0.8 3,322,589 - -2.2 3,322,600 - -1.3 3,322,600 - -0.3 3,322,620 - -0.3 3,322,620 - -0.5 3,322,620 - -1.3 3,322,635 + +0.2 3,322,639 + +0.7 3,322,639 + +0.1 3,322,640 - -1.5 3,322,640 - -0.2 3,322,665 - -0.9 3,322,665 - +0.0 3,322,674 + -0.8 3,322,674 + +1.5 3,322,674 + +1.0 3,322,674 + +0.4 3,322,675 - -1.1 3,322,675 - -0.9 3,322,675 - +0.3 3,322,675 - -0.3 3,322,675 - -0.6 3,322,704 + -0.9 3,322,704 + +0.2 3,322,704 + +0.2 3,322,705 - -1.6 3,322,705 - +0.8 3,322,705 - +0.1 3,322,800 + RR42_RS35080 0.12 +0.7 3,322,800 + RR42_RS35080 0.12 +0.3 3,322,800 + RR42_RS35080 0.12 -1.2 3,322,800 + RR42_RS35080 0.12 +0.0 3,322,801 - RR42_RS35080 0.12 -1.6 3,322,801 - RR42_RS35080 0.12 -1.7 3,322,927 - RR42_RS35080 0.26 +0.1 3,322,941 + RR42_RS35080 0.28 -0.2 3,322,941 + RR42_RS35080 0.28 -0.8 3,322,941 + RR42_RS35080 0.28 +1.9 3,322,941 + RR42_RS35080 0.28 -1.7 3,322,942 - RR42_RS35080 0.28 -1.0 3,322,942 - RR42_RS35080 0.28 -0.4 3,322,942 - RR42_RS35080 0.28 +0.0 3,322,944 + RR42_RS35080 0.28 +0.4 3,322,944 + RR42_RS35080 0.28 -0.8 3,322,944 + RR42_RS35080 0.28 +0.0 3,322,944 + RR42_RS35080 0.28 +0.7 3,322,944 + RR42_RS35080 0.28 -0.2 3,322,945 - RR42_RS35080 0.28 +0.4 3,322,945 - RR42_RS35080 0.28 -0.3 3,322,945 - RR42_RS35080 0.28 -1.1 3,322,945 - RR42_RS35080 0.28 -0.8 3,322,945 - RR42_RS35080 0.28 -0.3 3,322,945 - RR42_RS35080 0.28 -1.3 3,322,945 - RR42_RS35080 0.28 -0.3 3,322,953 + RR42_RS35080 0.29 +0.5 3,323,030 - RR42_RS35080 0.37 -0.9 3,323,030 - RR42_RS35080 0.37 -0.3 3,323,030 - RR42_RS35080 0.37 -0.3 3,323,101 + RR42_RS35080 0.45 +0.5 3,323,181 + RR42_RS35080 0.54 +0.0 3,323,190 + RR42_RS35080 0.55 +0.5 3,323,190 + RR42_RS35080 0.55 -1.5 3,323,190 + RR42_RS35080 0.55 +0.2 3,323,190 + RR42_RS35080 0.55 +0.4 3,323,191 - RR42_RS35080 0.55 -0.1 3,323,191 - RR42_RS35080 0.55 +0.9 3,323,191 - RR42_RS35080 0.55 +0.2 3,323,191 - RR42_RS35080 0.55 +0.0 3,323,247 + RR42_RS35080 0.62 +0.3 3,323,247 + RR42_RS35080 0.62 +0.6 3,323,248 - RR42_RS35080 0.62 -1.6 3,323,248 - RR42_RS35080 0.62 +0.3 3,323,288 - RR42_RS35080 0.66 -0.7 3,323,289 + RR42_RS35080 0.66 -0.3 3,323,289 + RR42_RS35080 0.66 -0.6 3,323,290 - RR42_RS35080 0.67 -0.1 3,323,290 - RR42_RS35080 0.67 -0.4 3,323,290 - RR42_RS35080 0.67 -0.3 3,323,290 - RR42_RS35080 0.67 -0.4 3,323,496 + RR42_RS35080 0.90 +0.0 3,323,497 - RR42_RS35080 0.90 -1.1
Or see this region's nucleotide sequence