Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34515 and RR42_RS34520 are separated by 190 nucleotides RR42_RS34520 and RR42_RS34525 are separated by 74 nucleotides
RR42_RS34515: RR42_RS34515 - acyltransferase, at 3,175,716 to 3,176,858
_RS34515
RR42_RS34520: RR42_RS34520 - transposase, at 3,177,049 to 3,177,483
_RS34520
RR42_RS34525: RR42_RS34525 - type VI secretion protein VasK, at 3,177,558 to 3,181,133
_RS34525
Position (kb)
3177
3178 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3176.104 kb on - strand, within RR42_RS34515 at 3176.157 kb on + strand, within RR42_RS34515 at 3176.158 kb on - strand, within RR42_RS34515 at 3176.158 kb on - strand, within RR42_RS34515 at 3176.361 kb on + strand, within RR42_RS34515 at 3176.361 kb on + strand, within RR42_RS34515 at 3176.382 kb on + strand, within RR42_RS34515 at 3176.382 kb on + strand, within RR42_RS34515 at 3176.490 kb on + strand, within RR42_RS34515 at 3176.490 kb on + strand, within RR42_RS34515 at 3176.541 kb on + strand, within RR42_RS34515 at 3176.555 kb on - strand, within RR42_RS34515 at 3176.555 kb on - strand, within RR42_RS34515 at 3176.555 kb on - strand, within RR42_RS34515 at 3176.598 kb on + strand, within RR42_RS34515 at 3176.605 kb on - strand, within RR42_RS34515 at 3176.613 kb on + strand, within RR42_RS34515 at 3176.613 kb on + strand, within RR42_RS34515 at 3176.614 kb on - strand, within RR42_RS34515 at 3176.614 kb on - strand, within RR42_RS34515 at 3176.914 kb on + strand at 3176.914 kb on + strand at 3177.009 kb on + strand at 3177.009 kb on + strand at 3177.010 kb on - strand at 3177.010 kb on - strand at 3177.013 kb on + strand at 3177.013 kb on + strand at 3177.014 kb on - strand at 3177.014 kb on - strand at 3177.015 kb on + strand at 3177.015 kb on + strand at 3177.015 kb on + strand at 3177.016 kb on - strand at 3177.016 kb on - strand at 3177.016 kb on - strand at 3177.428 kb on + strand, within RR42_RS34520 at 3177.472 kb on - strand at 3177.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,176,104 - RR42_RS34515 0.34 +0.7 3,176,157 + RR42_RS34515 0.39 -0.1 3,176,158 - RR42_RS34515 0.39 +1.8 3,176,158 - RR42_RS34515 0.39 +0.6 3,176,361 + RR42_RS34515 0.56 -0.6 3,176,361 + RR42_RS34515 0.56 -1.2 3,176,382 + RR42_RS34515 0.58 -0.1 3,176,382 + RR42_RS34515 0.58 -0.2 3,176,490 + RR42_RS34515 0.68 -0.5 3,176,490 + RR42_RS34515 0.68 -0.1 3,176,541 + RR42_RS34515 0.72 +0.6 3,176,555 - RR42_RS34515 0.73 +0.1 3,176,555 - RR42_RS34515 0.73 +1.4 3,176,555 - RR42_RS34515 0.73 +1.8 3,176,598 + RR42_RS34515 0.77 -0.3 3,176,605 - RR42_RS34515 0.78 -1.9 3,176,613 + RR42_RS34515 0.78 +0.0 3,176,613 + RR42_RS34515 0.78 +0.4 3,176,614 - RR42_RS34515 0.79 -0.1 3,176,614 - RR42_RS34515 0.79 +0.6 3,176,914 + +0.3 3,176,914 + +0.9 3,177,009 + -0.8 3,177,009 + -0.7 3,177,010 - +2.1 3,177,010 - -0.7 3,177,013 + -0.1 3,177,013 + -0.0 3,177,014 - -3.0 3,177,014 - +0.0 3,177,015 + -1.2 3,177,015 + +0.5 3,177,015 + -0.3 3,177,016 - -0.4 3,177,016 - -1.7 3,177,016 - +1.3 3,177,428 + RR42_RS34520 0.87 -1.2 3,177,472 - -1.3 3,177,472 - -0.0
Or see this region's nucleotide sequence