Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34205 and RR42_RS34210 are separated by 220 nucleotides RR42_RS34210 and RR42_RS34215 are separated by 257 nucleotides RR42_RS34215 and RR42_RS34220 are separated by 278 nucleotides
RR42_RS34205: RR42_RS34205 - hypothetical protein, at 3,099,530 to 3,099,880
_RS34205
RR42_RS34210: RR42_RS34210 - hypothetical protein, at 3,100,101 to 3,100,490
_RS34210
RR42_RS34215: RR42_RS34215 - ferredoxin, at 3,100,748 to 3,101,068
_RS34215
RR42_RS34220: RR42_RS34220 - pyruvate dehydrogenase, at 3,101,347 to 3,104,022
_RS34220
Position (kb)
3100
3101
3102 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3099.792 kb on - strand, within RR42_RS34205 at 3099.806 kb on + strand, within RR42_RS34205 at 3099.807 kb on - strand, within RR42_RS34205 at 3099.807 kb on - strand, within RR42_RS34205 at 3099.939 kb on + strand at 3100.270 kb on + strand, within RR42_RS34210 at 3100.271 kb on - strand, within RR42_RS34210 at 3100.271 kb on - strand, within RR42_RS34210 at 3100.496 kb on + strand at 3100.537 kb on - strand at 3100.582 kb on + strand at 3100.614 kb on - strand at 3100.730 kb on - strand at 3100.731 kb on + strand at 3100.731 kb on + strand at 3100.731 kb on + strand at 3100.893 kb on + strand, within RR42_RS34215 at 3101.308 kb on + strand at 3101.309 kb on - strand at 3101.547 kb on - strand at 3101.547 kb on - strand at 3101.852 kb on + strand, within RR42_RS34220 at 3101.853 kb on - strand, within RR42_RS34220 at 3101.906 kb on + strand, within RR42_RS34220 at 3101.907 kb on - strand, within RR42_RS34220 at 3101.907 kb on - strand, within RR42_RS34220 at 3101.930 kb on + strand, within RR42_RS34220 at 3101.931 kb on - strand, within RR42_RS34220 at 3101.931 kb on - strand, within RR42_RS34220 at 3101.933 kb on + strand, within RR42_RS34220 at 3101.933 kb on + strand, within RR42_RS34220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,099,792 - RR42_RS34205 0.75 -1.7 3,099,806 + RR42_RS34205 0.79 -0.1 3,099,807 - RR42_RS34205 0.79 +0.1 3,099,807 - RR42_RS34205 0.79 +0.1 3,099,939 + +0.5 3,100,270 + RR42_RS34210 0.43 -0.3 3,100,271 - RR42_RS34210 0.44 +0.2 3,100,271 - RR42_RS34210 0.44 -2.9 3,100,496 + -0.5 3,100,537 - -0.6 3,100,582 + +0.2 3,100,614 - +0.7 3,100,730 - +0.4 3,100,731 + -2.4 3,100,731 + +0.6 3,100,731 + +0.4 3,100,893 + RR42_RS34215 0.45 -1.0 3,101,308 + +0.1 3,101,309 - -0.5 3,101,547 - +1.8 3,101,547 - +0.7 3,101,852 + RR42_RS34220 0.19 -0.3 3,101,853 - RR42_RS34220 0.19 -0.2 3,101,906 + RR42_RS34220 0.21 -0.0 3,101,907 - RR42_RS34220 0.21 -1.5 3,101,907 - RR42_RS34220 0.21 +0.1 3,101,930 + RR42_RS34220 0.22 +1.1 3,101,931 - RR42_RS34220 0.22 +0.4 3,101,931 - RR42_RS34220 0.22 -0.2 3,101,933 + RR42_RS34220 0.22 +0.5 3,101,933 + RR42_RS34220 0.22 +0.4
Or see this region's nucleotide sequence