Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33795 and RR42_RS33800 overlap by 4 nucleotides RR42_RS33800 and RR42_RS33805 are separated by 136 nucleotides RR42_RS33805 and RR42_RS33810 are separated by 86 nucleotides RR42_RS33810 and RR42_RS33815 are separated by 55 nucleotides
RR42_RS33795: RR42_RS33795 - oxidase, at 3,011,880 to 3,013,136
_RS33795
RR42_RS33800: RR42_RS33800 - cytochrome C552, at 3,013,133 to 3,013,807
_RS33800
RR42_RS33805: RR42_RS33805 - 2-aminoadipate aminotransferase, at 3,013,944 to 3,015,458
_RS33805
RR42_RS33810: RR42_RS33810 - NIPSNAP family containing protein, at 3,015,545 to 3,015,850
_RS33810
RR42_RS33815: RR42_RS33815 - isochorismatase, at 3,015,906 to 3,016,460
_RS33815
Position (kb)
3013
3014
3015
3016 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3013.092 kb on + strand at 3013.154 kb on + strand at 3013.202 kb on + strand, within RR42_RS33800 at 3013.202 kb on + strand, within RR42_RS33800 at 3013.337 kb on + strand, within RR42_RS33800 at 3013.337 kb on + strand, within RR42_RS33800 at 3013.337 kb on + strand, within RR42_RS33800 at 3013.338 kb on - strand, within RR42_RS33800 at 3013.338 kb on - strand, within RR42_RS33800 at 3013.338 kb on - strand, within RR42_RS33800 at 3013.338 kb on - strand, within RR42_RS33800 at 3013.469 kb on + strand, within RR42_RS33800 at 3013.470 kb on - strand, within RR42_RS33800 at 3013.470 kb on - strand, within RR42_RS33800 at 3013.470 kb on - strand, within RR42_RS33800 at 3013.491 kb on - strand, within RR42_RS33800 at 3014.200 kb on - strand, within RR42_RS33805 at 3014.200 kb on - strand, within RR42_RS33805 at 3014.370 kb on + strand, within RR42_RS33805 at 3014.388 kb on + strand, within RR42_RS33805 at 3014.607 kb on + strand, within RR42_RS33805 at 3014.769 kb on + strand, within RR42_RS33805 at 3014.790 kb on + strand, within RR42_RS33805 at 3014.791 kb on - strand, within RR42_RS33805 at 3014.961 kb on + strand, within RR42_RS33805 at 3014.961 kb on + strand, within RR42_RS33805 at 3014.961 kb on + strand, within RR42_RS33805 at 3014.962 kb on - strand, within RR42_RS33805 at 3014.962 kb on - strand, within RR42_RS33805 at 3014.962 kb on - strand, within RR42_RS33805 at 3015.379 kb on - strand at 3015.546 kb on + strand at 3015.571 kb on + strand at 3015.572 kb on - strand at 3015.572 kb on - strand at 3015.579 kb on + strand, within RR42_RS33810 at 3015.580 kb on - strand, within RR42_RS33810 at 3015.580 kb on - strand, within RR42_RS33810 at 3015.658 kb on - strand, within RR42_RS33810 at 3015.658 kb on - strand, within RR42_RS33810 at 3015.666 kb on + strand, within RR42_RS33810 at 3015.735 kb on + strand, within RR42_RS33810 at 3015.736 kb on - strand, within RR42_RS33810 at 3015.736 kb on - strand, within RR42_RS33810 at 3015.736 kb on - strand, within RR42_RS33810 at 3015.736 kb on - strand, within RR42_RS33810 at 3015.826 kb on + strand at 3015.828 kb on + strand at 3015.828 kb on + strand at 3015.829 kb on - strand at 3015.829 kb on - strand at 3015.829 kb on - strand at 3015.829 kb on - strand at 3015.837 kb on + strand at 3016.193 kb on + strand, within RR42_RS33815 at 3016.431 kb on + strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.432 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,013,092 + -1.0 3,013,154 + -0.6 3,013,202 + RR42_RS33800 0.10 +0.7 3,013,202 + RR42_RS33800 0.10 +0.3 3,013,337 + RR42_RS33800 0.30 -0.4 3,013,337 + RR42_RS33800 0.30 -1.5 3,013,337 + RR42_RS33800 0.30 +0.1 3,013,338 - RR42_RS33800 0.30 -1.2 3,013,338 - RR42_RS33800 0.30 -0.3 3,013,338 - RR42_RS33800 0.30 +0.3 3,013,338 - RR42_RS33800 0.30 -1.6 3,013,469 + RR42_RS33800 0.50 -1.5 3,013,470 - RR42_RS33800 0.50 -0.0 3,013,470 - RR42_RS33800 0.50 -0.3 3,013,470 - RR42_RS33800 0.50 +0.7 3,013,491 - RR42_RS33800 0.53 -0.3 3,014,200 - RR42_RS33805 0.17 -1.6 3,014,200 - RR42_RS33805 0.17 -0.4 3,014,370 + RR42_RS33805 0.28 -1.7 3,014,388 + RR42_RS33805 0.29 -1.7 3,014,607 + RR42_RS33805 0.44 -0.7 3,014,769 + RR42_RS33805 0.54 +0.1 3,014,790 + RR42_RS33805 0.56 -0.4 3,014,791 - RR42_RS33805 0.56 +0.7 3,014,961 + RR42_RS33805 0.67 -2.0 3,014,961 + RR42_RS33805 0.67 -0.0 3,014,961 + RR42_RS33805 0.67 -1.1 3,014,962 - RR42_RS33805 0.67 -0.0 3,014,962 - RR42_RS33805 0.67 -0.7 3,014,962 - RR42_RS33805 0.67 +0.4 3,015,379 - -0.5 3,015,546 + +0.4 3,015,571 + +0.5 3,015,572 - -0.3 3,015,572 - -1.3 3,015,579 + RR42_RS33810 0.11 -0.6 3,015,580 - RR42_RS33810 0.11 +0.6 3,015,580 - RR42_RS33810 0.11 -0.7 3,015,658 - RR42_RS33810 0.37 -2.8 3,015,658 - RR42_RS33810 0.37 +0.5 3,015,666 + RR42_RS33810 0.40 +0.2 3,015,735 + RR42_RS33810 0.62 -1.9 3,015,736 - RR42_RS33810 0.62 +0.8 3,015,736 - RR42_RS33810 0.62 -0.4 3,015,736 - RR42_RS33810 0.62 +0.9 3,015,736 - RR42_RS33810 0.62 -1.3 3,015,826 + +0.3 3,015,828 + -0.8 3,015,828 + +0.8 3,015,829 - +0.4 3,015,829 - +1.3 3,015,829 - +0.1 3,015,829 - +0.5 3,015,837 + -2.4 3,016,193 + RR42_RS33815 0.52 +0.1 3,016,431 + +0.2 3,016,432 - +0.2 3,016,432 - +0.4 3,016,432 - -0.2 3,016,432 - -1.3 3,016,432 - +0.9 3,016,432 - -0.1
Or see this region's nucleotide sequence