Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33590 and RR42_RS33595 are separated by 11 nucleotides RR42_RS33595 and RR42_RS33600 overlap by 8 nucleotides RR42_RS33600 and RR42_RS33605 are separated by 135 nucleotides
RR42_RS33590: RR42_RS33590 - cyclase, at 2,964,378 to 2,965,136
_RS33590
RR42_RS33595: RR42_RS33595 - hypothetical protein, at 2,965,148 to 2,965,522
_RS33595
RR42_RS33600: RR42_RS33600 - oxidoreductase, at 2,965,515 to 2,966,303
_RS33600
RR42_RS33605: RR42_RS33605 - ABC transporter substrate-binding protein, at 2,966,439 to 2,967,716
_RS33605
Position (kb)
2965
2966
2967 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2964.655 kb on - strand, within RR42_RS33590 at 2964.730 kb on - strand, within RR42_RS33590 at 2964.733 kb on - strand, within RR42_RS33590 at 2964.739 kb on - strand, within RR42_RS33590 at 2964.753 kb on + strand, within RR42_RS33590 at 2964.753 kb on + strand, within RR42_RS33590 at 2964.754 kb on - strand, within RR42_RS33590 at 2964.754 kb on - strand, within RR42_RS33590 at 2964.892 kb on - strand, within RR42_RS33590 at 2964.892 kb on - strand, within RR42_RS33590 at 2965.078 kb on + strand at 2965.078 kb on + strand at 2965.078 kb on + strand at 2965.079 kb on - strand at 2965.325 kb on + strand, within RR42_RS33595 at 2965.326 kb on - strand, within RR42_RS33595 at 2965.326 kb on - strand, within RR42_RS33595 at 2965.498 kb on + strand at 2965.499 kb on - strand at 2965.499 kb on - strand at 2965.520 kb on + strand at 2965.520 kb on + strand at 2965.524 kb on - strand at 2965.555 kb on + strand at 2965.556 kb on - strand at 2965.556 kb on - strand at 2966.177 kb on + strand, within RR42_RS33600 at 2966.272 kb on + strand at 2966.273 kb on - strand at 2966.273 kb on - strand at 2966.274 kb on + strand at 2966.275 kb on - strand at 2966.275 kb on - strand at 2966.275 kb on - strand at 2966.290 kb on - strand at 2966.557 kb on - strand at 2966.741 kb on + strand, within RR42_RS33605 at 2966.741 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.863 kb on - strand, within RR42_RS33605 at 2966.865 kb on + strand, within RR42_RS33605 at 2966.866 kb on - strand, within RR42_RS33605 at 2966.910 kb on + strand, within RR42_RS33605 at 2966.910 kb on + strand, within RR42_RS33605 at 2966.910 kb on + strand, within RR42_RS33605 at 2966.911 kb on - strand, within RR42_RS33605 at 2966.949 kb on + strand, within RR42_RS33605 at 2966.949 kb on + strand, within RR42_RS33605 at 2966.950 kb on - strand, within RR42_RS33605 at 2966.950 kb on - strand, within RR42_RS33605
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,964,655 - RR42_RS33590 0.36 -0.3 2,964,730 - RR42_RS33590 0.46 +0.5 2,964,733 - RR42_RS33590 0.47 +1.8 2,964,739 - RR42_RS33590 0.48 +0.8 2,964,753 + RR42_RS33590 0.49 +0.1 2,964,753 + RR42_RS33590 0.49 +0.2 2,964,754 - RR42_RS33590 0.50 -1.3 2,964,754 - RR42_RS33590 0.50 -0.3 2,964,892 - RR42_RS33590 0.68 +0.6 2,964,892 - RR42_RS33590 0.68 -0.6 2,965,078 + +0.5 2,965,078 + +0.7 2,965,078 + -1.9 2,965,079 - +0.0 2,965,325 + RR42_RS33595 0.47 -0.0 2,965,326 - RR42_RS33595 0.47 -1.4 2,965,326 - RR42_RS33595 0.47 -1.5 2,965,498 + +1.9 2,965,499 - -2.3 2,965,499 - -1.3 2,965,520 + -1.2 2,965,520 + +0.3 2,965,524 - +0.1 2,965,555 + +0.6 2,965,556 - +3.3 2,965,556 - -0.3 2,966,177 + RR42_RS33600 0.84 -0.6 2,966,272 + -0.3 2,966,273 - -1.5 2,966,273 - -1.6 2,966,274 + +0.4 2,966,275 - -0.2 2,966,275 - -0.6 2,966,275 - -0.4 2,966,290 - +1.3 2,966,557 - -0.8 2,966,741 + RR42_RS33605 0.24 +0.5 2,966,741 + RR42_RS33605 0.24 +0.1 2,966,862 + RR42_RS33605 0.33 -0.7 2,966,862 + RR42_RS33605 0.33 +0.1 2,966,862 + RR42_RS33605 0.33 -1.0 2,966,862 + RR42_RS33605 0.33 +0.3 2,966,862 + RR42_RS33605 0.33 -0.3 2,966,862 + RR42_RS33605 0.33 +0.5 2,966,863 - RR42_RS33605 0.33 -0.3 2,966,865 + RR42_RS33605 0.33 +1.0 2,966,866 - RR42_RS33605 0.33 -0.4 2,966,910 + RR42_RS33605 0.37 +0.4 2,966,910 + RR42_RS33605 0.37 +2.2 2,966,910 + RR42_RS33605 0.37 -0.7 2,966,911 - RR42_RS33605 0.37 +0.4 2,966,949 + RR42_RS33605 0.40 +3.3 2,966,949 + RR42_RS33605 0.40 +0.1 2,966,950 - RR42_RS33605 0.40 +1.0 2,966,950 - RR42_RS33605 0.40 +0.5
Or see this region's nucleotide sequence