Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33580 and RR42_RS33585 are separated by 290 nucleotides RR42_RS33585 and RR42_RS33590 are separated by 104 nucleotides RR42_RS33590 and RR42_RS33595 are separated by 11 nucleotides
RR42_RS33580: RR42_RS33580 - 3-hydroxyacyl-CoA dehydrogenase, at 2,962,136 to 2,963,050
_RS33580
RR42_RS33585: RR42_RS33585 - 3-keto-5-aminohexanoate cleavage protein, at 2,963,341 to 2,964,273
_RS33585
RR42_RS33590: RR42_RS33590 - cyclase, at 2,964,378 to 2,965,136
_RS33590
RR42_RS33595: RR42_RS33595 - hypothetical protein, at 2,965,148 to 2,965,522
_RS33595
Position (kb)
2963
2964
2965 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2962.541 kb on + strand, within RR42_RS33580 at 2962.715 kb on + strand, within RR42_RS33580 at 2962.715 kb on + strand, within RR42_RS33580 at 2962.755 kb on - strand, within RR42_RS33580 at 2962.839 kb on - strand, within RR42_RS33580 at 2962.997 kb on + strand at 2962.997 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.411 kb on - strand at 2963.538 kb on + strand, within RR42_RS33585 at 2963.538 kb on + strand, within RR42_RS33585 at 2963.749 kb on + strand, within RR42_RS33585 at 2963.749 kb on + strand, within RR42_RS33585 at 2963.749 kb on + strand, within RR42_RS33585 at 2963.749 kb on + strand, within RR42_RS33585 at 2963.750 kb on - strand, within RR42_RS33585 at 2963.769 kb on + strand, within RR42_RS33585 at 2963.769 kb on + strand, within RR42_RS33585 at 2963.770 kb on - strand, within RR42_RS33585 at 2963.770 kb on - strand, within RR42_RS33585 at 2963.784 kb on + strand, within RR42_RS33585 at 2963.785 kb on - strand, within RR42_RS33585 at 2963.785 kb on - strand, within RR42_RS33585 at 2963.857 kb on + strand, within RR42_RS33585 at 2963.857 kb on + strand, within RR42_RS33585 at 2963.858 kb on - strand, within RR42_RS33585 at 2963.858 kb on - strand, within RR42_RS33585 at 2963.858 kb on - strand, within RR42_RS33585 at 2963.864 kb on + strand, within RR42_RS33585 at 2963.864 kb on + strand, within RR42_RS33585 at 2963.864 kb on + strand, within RR42_RS33585 at 2963.864 kb on + strand, within RR42_RS33585 at 2963.875 kb on + strand, within RR42_RS33585 at 2964.000 kb on + strand, within RR42_RS33585 at 2964.022 kb on + strand, within RR42_RS33585 at 2964.023 kb on - strand, within RR42_RS33585 at 2964.023 kb on - strand, within RR42_RS33585 at 2964.025 kb on + strand, within RR42_RS33585 at 2964.025 kb on + strand, within RR42_RS33585 at 2964.026 kb on - strand, within RR42_RS33585 at 2964.074 kb on - strand, within RR42_RS33585 at 2964.123 kb on + strand, within RR42_RS33585 at 2964.124 kb on + strand, within RR42_RS33585 at 2964.125 kb on - strand, within RR42_RS33585 at 2964.125 kb on - strand, within RR42_RS33585 at 2964.286 kb on + strand at 2964.286 kb on + strand at 2964.331 kb on - strand at 2964.407 kb on + strand at 2964.485 kb on + strand, within RR42_RS33590 at 2964.506 kb on + strand, within RR42_RS33590 at 2964.655 kb on - strand, within RR42_RS33590 at 2964.730 kb on - strand, within RR42_RS33590 at 2964.733 kb on - strand, within RR42_RS33590 at 2964.739 kb on - strand, within RR42_RS33590 at 2964.753 kb on + strand, within RR42_RS33590 at 2964.753 kb on + strand, within RR42_RS33590 at 2964.754 kb on - strand, within RR42_RS33590 at 2964.754 kb on - strand, within RR42_RS33590 at 2964.892 kb on - strand, within RR42_RS33590 at 2964.892 kb on - strand, within RR42_RS33590 at 2965.078 kb on + strand at 2965.078 kb on + strand at 2965.078 kb on + strand at 2965.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,962,541 + RR42_RS33580 0.44 +0.9 2,962,715 + RR42_RS33580 0.63 +0.8 2,962,715 + RR42_RS33580 0.63 +0.3 2,962,755 - RR42_RS33580 0.68 +0.9 2,962,839 - RR42_RS33580 0.77 +0.8 2,962,997 + +0.0 2,962,997 + -1.6 2,963,044 + -0.8 2,963,044 + -1.3 2,963,044 + -1.2 2,963,044 + -0.1 2,963,044 + -1.8 2,963,044 + -0.2 2,963,044 + -0.1 2,963,045 - -0.6 2,963,045 - -0.0 2,963,045 - +0.2 2,963,045 - +0.9 2,963,045 - +0.1 2,963,045 - +0.3 2,963,045 - -0.1 2,963,411 - -0.4 2,963,538 + RR42_RS33585 0.21 +0.3 2,963,538 + RR42_RS33585 0.21 -0.0 2,963,749 + RR42_RS33585 0.44 -0.7 2,963,749 + RR42_RS33585 0.44 -0.1 2,963,749 + RR42_RS33585 0.44 +0.2 2,963,749 + RR42_RS33585 0.44 -3.2 2,963,750 - RR42_RS33585 0.44 -0.1 2,963,769 + RR42_RS33585 0.46 +0.2 2,963,769 + RR42_RS33585 0.46 +0.7 2,963,770 - RR42_RS33585 0.46 +0.1 2,963,770 - RR42_RS33585 0.46 +0.4 2,963,784 + RR42_RS33585 0.47 -1.3 2,963,785 - RR42_RS33585 0.48 -0.4 2,963,785 - RR42_RS33585 0.48 +0.6 2,963,857 + RR42_RS33585 0.55 -0.3 2,963,857 + RR42_RS33585 0.55 +0.3 2,963,858 - RR42_RS33585 0.55 -0.7 2,963,858 - RR42_RS33585 0.55 +0.1 2,963,858 - RR42_RS33585 0.55 -1.9 2,963,864 + RR42_RS33585 0.56 -0.6 2,963,864 + RR42_RS33585 0.56 +1.8 2,963,864 + RR42_RS33585 0.56 +0.1 2,963,864 + RR42_RS33585 0.56 -0.8 2,963,875 + RR42_RS33585 0.57 +1.9 2,964,000 + RR42_RS33585 0.71 -0.2 2,964,022 + RR42_RS33585 0.73 -1.6 2,964,023 - RR42_RS33585 0.73 +1.3 2,964,023 - RR42_RS33585 0.73 +1.1 2,964,025 + RR42_RS33585 0.73 -0.6 2,964,025 + RR42_RS33585 0.73 +0.2 2,964,026 - RR42_RS33585 0.73 -0.4 2,964,074 - RR42_RS33585 0.79 -0.2 2,964,123 + RR42_RS33585 0.84 -0.5 2,964,124 + RR42_RS33585 0.84 +0.3 2,964,125 - RR42_RS33585 0.84 +0.3 2,964,125 - RR42_RS33585 0.84 -0.8 2,964,286 + +0.1 2,964,286 + +0.4 2,964,331 - +1.1 2,964,407 + -0.1 2,964,485 + RR42_RS33590 0.14 -0.2 2,964,506 + RR42_RS33590 0.17 -0.4 2,964,655 - RR42_RS33590 0.36 -0.3 2,964,730 - RR42_RS33590 0.46 +0.5 2,964,733 - RR42_RS33590 0.47 +1.8 2,964,739 - RR42_RS33590 0.48 +0.8 2,964,753 + RR42_RS33590 0.49 +0.1 2,964,753 + RR42_RS33590 0.49 +0.2 2,964,754 - RR42_RS33590 0.50 -1.3 2,964,754 - RR42_RS33590 0.50 -0.3 2,964,892 - RR42_RS33590 0.68 +0.6 2,964,892 - RR42_RS33590 0.68 -0.6 2,965,078 + +0.5 2,965,078 + +0.7 2,965,078 + -1.9 2,965,079 - +0.0
Or see this region's nucleotide sequence