Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33440 and RR42_RS33445 are separated by 131 nucleotides RR42_RS33445 and RR42_RS33450 are separated by 72 nucleotides
RR42_RS33440: RR42_RS33440 - LysR family transcriptional regulator, at 2,921,440 to 2,922,369
_RS33440
RR42_RS33445: RR42_RS33445 - haloacid dehalogenase, at 2,922,501 to 2,923,151
_RS33445
RR42_RS33450: RR42_RS33450 - alanine racemase, at 2,923,224 to 2,924,378
_RS33450
Position (kb)
2922
2923
2924 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2921.503 kb on + strand at 2921.691 kb on + strand, within RR42_RS33440 at 2921.861 kb on + strand, within RR42_RS33440 at 2921.941 kb on - strand, within RR42_RS33440 at 2921.941 kb on - strand, within RR42_RS33440 at 2922.418 kb on + strand at 2922.419 kb on - strand at 2922.421 kb on + strand at 2922.421 kb on + strand at 2922.422 kb on - strand at 2922.422 kb on - strand at 2922.429 kb on - strand at 2922.500 kb on + strand at 2922.500 kb on + strand at 2922.500 kb on + strand at 2922.500 kb on + strand at 2922.501 kb on - strand at 2922.501 kb on - strand at 2922.501 kb on - strand at 2922.589 kb on + strand, within RR42_RS33445 at 2922.589 kb on + strand, within RR42_RS33445 at 2922.590 kb on - strand, within RR42_RS33445 at 2922.643 kb on - strand, within RR42_RS33445 at 2922.657 kb on + strand, within RR42_RS33445 at 2922.657 kb on + strand, within RR42_RS33445 at 2922.657 kb on + strand, within RR42_RS33445 at 2922.657 kb on + strand, within RR42_RS33445 at 2922.658 kb on - strand, within RR42_RS33445 at 2922.672 kb on + strand, within RR42_RS33445 at 2922.681 kb on + strand, within RR42_RS33445 at 2922.924 kb on + strand, within RR42_RS33445 at 2922.924 kb on + strand, within RR42_RS33445 at 2922.925 kb on - strand, within RR42_RS33445 at 2922.925 kb on - strand, within RR42_RS33445 at 2922.925 kb on - strand, within RR42_RS33445 at 2922.925 kb on - strand, within RR42_RS33445 at 2922.946 kb on - strand, within RR42_RS33445 at 2923.050 kb on + strand, within RR42_RS33445 at 2923.050 kb on + strand, within RR42_RS33445 at 2923.223 kb on + strand at 2923.223 kb on + strand at 2923.224 kb on - strand at 2923.770 kb on - strand, within RR42_RS33450 at 2923.941 kb on + strand, within RR42_RS33450 at 2923.942 kb on - strand, within RR42_RS33450
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,921,503 + -1.7 2,921,691 + RR42_RS33440 0.27 +0.9 2,921,861 + RR42_RS33440 0.45 +0.3 2,921,941 - RR42_RS33440 0.54 +0.3 2,921,941 - RR42_RS33440 0.54 +0.8 2,922,418 + -1.2 2,922,419 - -1.0 2,922,421 + +0.6 2,922,421 + -0.0 2,922,422 - -1.0 2,922,422 - -0.8 2,922,429 - +1.4 2,922,500 + +1.5 2,922,500 + -1.9 2,922,500 + -0.5 2,922,500 + -2.7 2,922,501 - +0.5 2,922,501 - -0.5 2,922,501 - -0.1 2,922,589 + RR42_RS33445 0.14 +0.5 2,922,589 + RR42_RS33445 0.14 -0.3 2,922,590 - RR42_RS33445 0.14 -0.1 2,922,643 - RR42_RS33445 0.22 +0.4 2,922,657 + RR42_RS33445 0.24 +0.6 2,922,657 + RR42_RS33445 0.24 -0.5 2,922,657 + RR42_RS33445 0.24 +0.7 2,922,657 + RR42_RS33445 0.24 -2.2 2,922,658 - RR42_RS33445 0.24 +0.8 2,922,672 + RR42_RS33445 0.26 -0.2 2,922,681 + RR42_RS33445 0.28 +0.3 2,922,924 + RR42_RS33445 0.65 +0.0 2,922,924 + RR42_RS33445 0.65 -0.4 2,922,925 - RR42_RS33445 0.65 +0.5 2,922,925 - RR42_RS33445 0.65 -0.2 2,922,925 - RR42_RS33445 0.65 -0.1 2,922,925 - RR42_RS33445 0.65 -0.0 2,922,946 - RR42_RS33445 0.68 +0.6 2,923,050 + RR42_RS33445 0.84 -1.4 2,923,050 + RR42_RS33445 0.84 -0.4 2,923,223 + -0.3 2,923,223 + +1.9 2,923,224 - -0.6 2,923,770 - RR42_RS33450 0.47 +0.0 2,923,941 + RR42_RS33450 0.62 +0.4 2,923,942 - RR42_RS33450 0.62 +0.2
Or see this region's nucleotide sequence