Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33150 and RR42_RS33155 are separated by 109 nucleotides RR42_RS33155 and RR42_RS33160 are separated by 95 nucleotides RR42_RS33160 and RR42_RS33165 are separated by 200 nucleotides
RR42_RS33150: RR42_RS33150 - hypothetical protein, at 2,843,522 to 2,843,872
_RS33150
RR42_RS33155: RR42_RS33155 - hypothetical protein, at 2,843,982 to 2,844,161
_RS33155
RR42_RS33160: RR42_RS33160 - nitrogen fixation protein NifQ, at 2,844,257 to 2,844,829
_RS33160
RR42_RS33165: RR42_RS33165 - acyl-CoA thioesterase, at 2,845,030 to 2,845,863
_RS33165
Position (kb)
2844
2845 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2843.564 kb on + strand, within RR42_RS33150 at 2843.565 kb on - strand, within RR42_RS33150 at 2843.565 kb on - strand, within RR42_RS33150 at 2843.565 kb on - strand, within RR42_RS33150 at 2843.894 kb on + strand at 2843.950 kb on + strand at 2843.950 kb on + strand at 2843.951 kb on - strand at 2844.018 kb on + strand, within RR42_RS33155 at 2844.018 kb on + strand, within RR42_RS33155 at 2844.019 kb on - strand, within RR42_RS33155 at 2844.019 kb on - strand, within RR42_RS33155 at 2844.047 kb on + strand, within RR42_RS33155 at 2844.120 kb on + strand, within RR42_RS33155 at 2844.120 kb on + strand, within RR42_RS33155 at 2844.120 kb on + strand, within RR42_RS33155 at 2844.120 kb on + strand, within RR42_RS33155 at 2844.121 kb on - strand, within RR42_RS33155 at 2844.121 kb on - strand, within RR42_RS33155 at 2844.121 kb on - strand, within RR42_RS33155 at 2844.121 kb on - strand, within RR42_RS33155 at 2844.159 kb on + strand at 2844.219 kb on + strand at 2844.220 kb on - strand at 2844.220 kb on - strand at 2844.222 kb on + strand at 2844.300 kb on + strand at 2844.300 kb on + strand at 2844.301 kb on - strand at 2844.301 kb on - strand at 2844.301 kb on - strand at 2844.359 kb on + strand, within RR42_RS33160 at 2844.359 kb on + strand, within RR42_RS33160 at 2844.369 kb on + strand, within RR42_RS33160 at 2844.370 kb on - strand, within RR42_RS33160 at 2844.411 kb on + strand, within RR42_RS33160 at 2844.426 kb on + strand, within RR42_RS33160 at 2844.426 kb on + strand, within RR42_RS33160 at 2844.426 kb on + strand, within RR42_RS33160 at 2844.427 kb on - strand, within RR42_RS33160 at 2844.575 kb on + strand, within RR42_RS33160 at 2844.901 kb on - strand at 2844.901 kb on - strand at 2844.910 kb on + strand at 2844.967 kb on + strand at 2844.967 kb on + strand at 2844.971 kb on + strand at 2844.971 kb on + strand at 2844.971 kb on + strand at 2844.972 kb on - strand at 2844.972 kb on - strand at 2844.972 kb on - strand at 2844.972 kb on - strand at 2844.972 kb on - strand at 2845.026 kb on + strand at 2845.026 kb on + strand at 2845.026 kb on + strand at 2845.026 kb on + strand at 2845.027 kb on - strand at 2845.031 kb on + strand at 2845.031 kb on + strand at 2845.032 kb on - strand at 2845.073 kb on + strand at 2845.073 kb on + strand at 2845.074 kb on - strand at 2845.292 kb on + strand, within RR42_RS33165 at 2845.523 kb on + strand, within RR42_RS33165 at 2845.721 kb on + strand, within RR42_RS33165 at 2845.722 kb on - strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.725 kb on + strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165 at 2845.726 kb on - strand, within RR42_RS33165
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,843,564 + RR42_RS33150 0.12 -0.7 2,843,565 - RR42_RS33150 0.12 -0.2 2,843,565 - RR42_RS33150 0.12 +1.7 2,843,565 - RR42_RS33150 0.12 -0.9 2,843,894 + +1.6 2,843,950 + -0.6 2,843,950 + -0.4 2,843,951 - +0.2 2,844,018 + RR42_RS33155 0.20 -0.3 2,844,018 + RR42_RS33155 0.20 +0.2 2,844,019 - RR42_RS33155 0.21 +0.0 2,844,019 - RR42_RS33155 0.21 -0.1 2,844,047 + RR42_RS33155 0.36 -0.8 2,844,120 + RR42_RS33155 0.77 +0.3 2,844,120 + RR42_RS33155 0.77 -0.3 2,844,120 + RR42_RS33155 0.77 +0.8 2,844,120 + RR42_RS33155 0.77 +0.8 2,844,121 - RR42_RS33155 0.77 -0.5 2,844,121 - RR42_RS33155 0.77 -0.2 2,844,121 - RR42_RS33155 0.77 +0.8 2,844,121 - RR42_RS33155 0.77 -0.5 2,844,159 + +0.7 2,844,219 + +0.5 2,844,220 - -0.9 2,844,220 - -0.3 2,844,222 + -0.2 2,844,300 + -2.0 2,844,300 + +0.1 2,844,301 - +0.1 2,844,301 - -0.7 2,844,301 - -0.3 2,844,359 + RR42_RS33160 0.18 -0.1 2,844,359 + RR42_RS33160 0.18 +0.2 2,844,369 + RR42_RS33160 0.20 -0.3 2,844,370 - RR42_RS33160 0.20 -0.1 2,844,411 + RR42_RS33160 0.27 +0.5 2,844,426 + RR42_RS33160 0.29 +0.6 2,844,426 + RR42_RS33160 0.29 -0.3 2,844,426 + RR42_RS33160 0.29 +0.1 2,844,427 - RR42_RS33160 0.30 +0.4 2,844,575 + RR42_RS33160 0.55 -0.6 2,844,901 - +0.5 2,844,901 - -0.5 2,844,910 + -1.0 2,844,967 + -0.8 2,844,967 + +0.4 2,844,971 + +0.6 2,844,971 + +0.5 2,844,971 + -0.0 2,844,972 - -0.2 2,844,972 - -0.4 2,844,972 - -0.8 2,844,972 - -2.0 2,844,972 - +0.1 2,845,026 + -0.0 2,845,026 + +0.2 2,845,026 + -0.2 2,845,026 + +0.2 2,845,027 - +0.5 2,845,031 + +0.5 2,845,031 + -0.0 2,845,032 - +0.3 2,845,073 + +0.1 2,845,073 + +0.3 2,845,074 - -0.1 2,845,292 + RR42_RS33165 0.31 -0.0 2,845,523 + RR42_RS33165 0.59 +0.3 2,845,721 + RR42_RS33165 0.83 -0.7 2,845,722 - RR42_RS33165 0.83 +0.7 2,845,725 + RR42_RS33165 0.83 +1.5 2,845,725 + RR42_RS33165 0.83 +0.5 2,845,725 + RR42_RS33165 0.83 -0.2 2,845,725 + RR42_RS33165 0.83 +0.9 2,845,725 + RR42_RS33165 0.83 +1.0 2,845,726 - RR42_RS33165 0.83 +2.3 2,845,726 - RR42_RS33165 0.83 -1.6 2,845,726 - RR42_RS33165 0.83 +0.8 2,845,726 - RR42_RS33165 0.83 +0.4 2,845,726 - RR42_RS33165 0.83 +1.0 2,845,726 - RR42_RS33165 0.83 -0.8 2,845,726 - RR42_RS33165 0.83 +0.2 2,845,726 - RR42_RS33165 0.83 -0.4
Or see this region's nucleotide sequence