Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32985 and RR42_RS32990 are separated by 153 nucleotides RR42_RS32990 and RR42_RS32995 are separated by 223 nucleotides RR42_RS32995 and RR42_RS33000 are separated by 43 nucleotides
RR42_RS32985: RR42_RS32985 - ABC transporter substrate-binding protein, at 2,801,502 to 2,802,491
_RS32985
RR42_RS32990: RR42_RS32990 - 1-aminocyclopropane-1-carboxylate deaminase, at 2,802,645 to 2,803,661
_RS32990
RR42_RS32995: RR42_RS32995 - AsnC family transcriptional regulator, at 2,803,885 to 2,804,394
_RS32995
RR42_RS33000: RR42_RS33000 - hypothetical protein, at 2,804,438 to 2,806,012
_RS33000
Position (kb)
2802
2803
2804 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2801.838 kb on - strand, within RR42_RS32985 at 2801.924 kb on - strand, within RR42_RS32985 at 2801.924 kb on - strand, within RR42_RS32985 at 2801.924 kb on - strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.931 kb on - strand, within RR42_RS32985 at 2801.931 kb on - strand, within RR42_RS32985 at 2801.931 kb on - strand, within RR42_RS32985 at 2801.931 kb on - strand, within RR42_RS32985 at 2802.016 kb on + strand, within RR42_RS32985 at 2802.017 kb on - strand, within RR42_RS32985 at 2802.212 kb on + strand, within RR42_RS32985 at 2802.212 kb on + strand, within RR42_RS32985 at 2802.212 kb on + strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.215 kb on - strand, within RR42_RS32985 at 2802.215 kb on - strand, within RR42_RS32985 at 2802.496 kb on + strand at 2802.668 kb on + strand at 2802.668 kb on + strand at 2802.668 kb on + strand at 2802.669 kb on - strand at 2802.670 kb on + strand at 2802.707 kb on - strand at 2802.707 kb on - strand at 2802.707 kb on - strand at 2802.875 kb on - strand, within RR42_RS32990 at 2803.087 kb on + strand, within RR42_RS32990 at 2803.088 kb on - strand, within RR42_RS32990 at 2803.088 kb on - strand, within RR42_RS32990 at 2803.333 kb on + strand, within RR42_RS32990 at 2803.333 kb on + strand, within RR42_RS32990 at 2803.333 kb on + strand, within RR42_RS32990 at 2803.348 kb on + strand, within RR42_RS32990 at 2803.348 kb on + strand, within RR42_RS32990 at 2803.492 kb on + strand, within RR42_RS32990 at 2803.493 kb on - strand, within RR42_RS32990 at 2803.493 kb on - strand, within RR42_RS32990 at 2803.493 kb on - strand, within RR42_RS32990 at 2803.559 kb on - strand, within RR42_RS32990 at 2803.956 kb on + strand, within RR42_RS32995 at 2804.251 kb on + strand, within RR42_RS32995 at 2804.251 kb on + strand, within RR42_RS32995 at 2804.252 kb on - strand, within RR42_RS32995 at 2804.318 kb on - strand, within RR42_RS32995 at 2804.318 kb on - strand, within RR42_RS32995 at 2804.339 kb on + strand, within RR42_RS32995 at 2804.339 kb on + strand, within RR42_RS32995 at 2804.339 kb on + strand, within RR42_RS32995 at 2804.340 kb on - strand, within RR42_RS32995 at 2804.340 kb on - strand, within RR42_RS32995 at 2804.440 kb on - strand at 2804.536 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,801,838 - RR42_RS32985 0.34 +1.1 2,801,924 - RR42_RS32985 0.43 +0.0 2,801,924 - RR42_RS32985 0.43 -0.5 2,801,924 - RR42_RS32985 0.43 +0.6 2,801,930 + RR42_RS32985 0.43 +0.7 2,801,930 + RR42_RS32985 0.43 -0.1 2,801,930 + RR42_RS32985 0.43 -0.9 2,801,930 + RR42_RS32985 0.43 -0.3 2,801,930 + RR42_RS32985 0.43 -1.1 2,801,930 + RR42_RS32985 0.43 +0.0 2,801,931 - RR42_RS32985 0.43 +1.0 2,801,931 - RR42_RS32985 0.43 +1.8 2,801,931 - RR42_RS32985 0.43 -0.9 2,801,931 - RR42_RS32985 0.43 -0.5 2,802,016 + RR42_RS32985 0.52 -0.3 2,802,017 - RR42_RS32985 0.52 +0.8 2,802,212 + RR42_RS32985 0.72 -0.5 2,802,212 + RR42_RS32985 0.72 +0.7 2,802,212 + RR42_RS32985 0.72 +2.3 2,802,213 - RR42_RS32985 0.72 -0.6 2,802,213 - RR42_RS32985 0.72 -0.7 2,802,213 - RR42_RS32985 0.72 +1.0 2,802,213 - RR42_RS32985 0.72 +0.7 2,802,213 - RR42_RS32985 0.72 -2.6 2,802,215 - RR42_RS32985 0.72 +2.5 2,802,215 - RR42_RS32985 0.72 +1.0 2,802,496 + -0.3 2,802,668 + +0.6 2,802,668 + +0.1 2,802,668 + +0.1 2,802,669 - +0.6 2,802,670 + +0.8 2,802,707 - -1.6 2,802,707 - -0.7 2,802,707 - +0.1 2,802,875 - RR42_RS32990 0.23 +1.7 2,803,087 + RR42_RS32990 0.43 -0.7 2,803,088 - RR42_RS32990 0.44 +0.3 2,803,088 - RR42_RS32990 0.44 +0.2 2,803,333 + RR42_RS32990 0.68 +1.0 2,803,333 + RR42_RS32990 0.68 -1.7 2,803,333 + RR42_RS32990 0.68 +0.1 2,803,348 + RR42_RS32990 0.69 -0.6 2,803,348 + RR42_RS32990 0.69 +1.1 2,803,492 + RR42_RS32990 0.83 +0.5 2,803,493 - RR42_RS32990 0.83 +1.1 2,803,493 - RR42_RS32990 0.83 +0.2 2,803,493 - RR42_RS32990 0.83 -0.0 2,803,559 - RR42_RS32990 0.90 +1.3 2,803,956 + RR42_RS32995 0.14 -0.7 2,804,251 + RR42_RS32995 0.72 -1.0 2,804,251 + RR42_RS32995 0.72 -1.3 2,804,252 - RR42_RS32995 0.72 +0.7 2,804,318 - RR42_RS32995 0.85 +1.3 2,804,318 - RR42_RS32995 0.85 -0.6 2,804,339 + RR42_RS32995 0.89 +0.3 2,804,339 + RR42_RS32995 0.89 +0.8 2,804,339 + RR42_RS32995 0.89 -1.7 2,804,340 - RR42_RS32995 0.89 -0.8 2,804,340 - RR42_RS32995 0.89 -0.2 2,804,440 - -1.2 2,804,536 - -0.6
Or see this region's nucleotide sequence