Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32400 and RR42_RS32405 are separated by 203 nucleotides RR42_RS32405 and RR42_RS32410 overlap by 4 nucleotides RR42_RS32410 and RR42_RS32415 are separated by 912 nucleotides
RR42_RS32400: RR42_RS32400 - hypothetical protein, at 2,664,081 to 2,664,551
_RS32400
RR42_RS32405: RR42_RS32405 - anti-sigma factor, at 2,664,755 to 2,665,546
_RS32405
RR42_RS32410: RR42_RS32410 - RNA polymerase subunit sigma-24, at 2,665,543 to 2,666,091
_RS32410
RR42_RS32415: RR42_RS32415 - hemolysin D, at 2,667,004 to 2,668,236
_RS32415
Position (kb)
2665
2666
2667 Strain fitness (log2 ratio)
-2
-1
0
1 at 2664.722 kb on + strand at 2664.756 kb on + strand at 2664.756 kb on + strand at 2664.756 kb on + strand at 2664.757 kb on - strand at 2664.757 kb on - strand at 2664.757 kb on - strand at 2664.873 kb on + strand, within RR42_RS32405 at 2664.942 kb on + strand, within RR42_RS32405 at 2664.942 kb on + strand, within RR42_RS32405 at 2664.942 kb on + strand, within RR42_RS32405 at 2664.943 kb on - strand, within RR42_RS32405 at 2664.943 kb on - strand, within RR42_RS32405 at 2664.975 kb on + strand, within RR42_RS32405 at 2664.976 kb on - strand, within RR42_RS32405 at 2664.976 kb on - strand, within RR42_RS32405 at 2665.164 kb on + strand, within RR42_RS32405 at 2665.164 kb on + strand, within RR42_RS32405 at 2665.164 kb on + strand, within RR42_RS32405 at 2665.165 kb on - strand, within RR42_RS32405 at 2665.165 kb on - strand, within RR42_RS32405 at 2665.165 kb on - strand, within RR42_RS32405 at 2665.165 kb on - strand, within RR42_RS32405 at 2665.567 kb on + strand at 2665.574 kb on - strand at 2665.574 kb on - strand at 2665.898 kb on - strand, within RR42_RS32410 at 2665.901 kb on + strand, within RR42_RS32410 at 2666.042 kb on + strand at 2666.528 kb on + strand at 2666.528 kb on + strand at 2666.529 kb on - strand at 2667.033 kb on + strand at 2667.033 kb on + strand at 2667.034 kb on - strand at 2667.057 kb on - strand at 2667.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,664,722 + +0.3 2,664,756 + +0.1 2,664,756 + -0.1 2,664,756 + -0.6 2,664,757 - +0.2 2,664,757 - -0.1 2,664,757 - +0.1 2,664,873 + RR42_RS32405 0.15 +0.0 2,664,942 + RR42_RS32405 0.24 -0.7 2,664,942 + RR42_RS32405 0.24 +0.4 2,664,942 + RR42_RS32405 0.24 -0.1 2,664,943 - RR42_RS32405 0.24 -0.1 2,664,943 - RR42_RS32405 0.24 -0.7 2,664,975 + RR42_RS32405 0.28 -0.7 2,664,976 - RR42_RS32405 0.28 -0.3 2,664,976 - RR42_RS32405 0.28 +0.2 2,665,164 + RR42_RS32405 0.52 +0.4 2,665,164 + RR42_RS32405 0.52 -1.1 2,665,164 + RR42_RS32405 0.52 +1.5 2,665,165 - RR42_RS32405 0.52 +1.1 2,665,165 - RR42_RS32405 0.52 -0.8 2,665,165 - RR42_RS32405 0.52 +0.3 2,665,165 - RR42_RS32405 0.52 -0.1 2,665,567 + -1.3 2,665,574 - -0.2 2,665,574 - -0.3 2,665,898 - RR42_RS32410 0.65 +1.3 2,665,901 + RR42_RS32410 0.65 +1.3 2,666,042 + +0.5 2,666,528 + -1.6 2,666,528 + -0.0 2,666,529 - +1.0 2,667,033 + -0.1 2,667,033 + -1.0 2,667,034 - -0.3 2,667,057 - -0.9 2,667,057 - -2.1
Or see this region's nucleotide sequence