Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32230 and RR42_RS32235 are separated by 33 nucleotides RR42_RS32235 and RR42_RS32240 are separated by 235 nucleotides
RR42_RS32230: RR42_RS32230 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 2,617,395 to 2,618,396
_RS32230
RR42_RS32235: RR42_RS32235 - hopanoid biosynthesis associated radical SAM protein HpnH, at 2,618,430 to 2,619,596
_RS32235
RR42_RS32240: RR42_RS32240 - ABC transporter, at 2,619,832 to 2,620,479
_RS32240
Position (kb)
2618
2619
2620 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2617.459 kb on + strand at 2617.460 kb on - strand at 2617.460 kb on - strand at 2617.719 kb on + strand, within RR42_RS32230 at 2617.720 kb on - strand, within RR42_RS32230 at 2617.873 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.910 kb on + strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.913 kb on + strand, within RR42_RS32230 at 2617.913 kb on + strand, within RR42_RS32230 at 2617.914 kb on - strand, within RR42_RS32230 at 2617.914 kb on - strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.916 kb on - strand, within RR42_RS32230 at 2617.916 kb on - strand, within RR42_RS32230 at 2617.943 kb on + strand, within RR42_RS32230 at 2617.977 kb on + strand, within RR42_RS32230 at 2618.058 kb on - strand, within RR42_RS32230 at 2618.058 kb on - strand, within RR42_RS32230 at 2618.112 kb on + strand, within RR42_RS32230 at 2618.413 kb on + strand at 2618.425 kb on - strand at 2618.470 kb on - strand at 2618.470 kb on - strand at 2618.555 kb on + strand, within RR42_RS32235 at 2618.784 kb on + strand, within RR42_RS32235 at 2618.785 kb on - strand, within RR42_RS32235 at 2618.872 kb on - strand, within RR42_RS32235 at 2619.030 kb on + strand, within RR42_RS32235 at 2619.186 kb on + strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.265 kb on - strand, within RR42_RS32235 at 2619.312 kb on + strand, within RR42_RS32235 at 2619.313 kb on - strand, within RR42_RS32235 at 2619.357 kb on - strand, within RR42_RS32235 at 2619.438 kb on - strand, within RR42_RS32235 at 2619.462 kb on - strand, within RR42_RS32235 at 2619.492 kb on + strand at 2619.493 kb on - strand at 2619.493 kb on - strand at 2619.759 kb on - strand at 2619.872 kb on + strand at 2619.873 kb on - strand at 2619.873 kb on - strand at 2619.873 kb on - strand at 2619.909 kb on + strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.969 kb on - strand, within RR42_RS32240 at 2619.969 kb on - strand, within RR42_RS32240 at 2620.092 kb on - strand, within RR42_RS32240 at 2620.092 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.172 kb on + strand, within RR42_RS32240 at 2620.173 kb on - strand, within RR42_RS32240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,617,459 + +0.0 2,617,460 - +0.8 2,617,460 - -2.4 2,617,719 + RR42_RS32230 0.32 -0.1 2,617,720 - RR42_RS32230 0.32 +0.1 2,617,873 - RR42_RS32230 0.48 -0.2 2,617,887 - RR42_RS32230 0.49 -0.8 2,617,887 - RR42_RS32230 0.49 -0.7 2,617,887 - RR42_RS32230 0.49 -1.9 2,617,910 + RR42_RS32230 0.51 +0.1 2,617,911 - RR42_RS32230 0.51 -3.0 2,617,911 - RR42_RS32230 0.51 +0.3 2,617,911 - RR42_RS32230 0.51 -1.5 2,617,911 - RR42_RS32230 0.51 -1.2 2,617,913 + RR42_RS32230 0.52 -0.9 2,617,913 + RR42_RS32230 0.52 -0.1 2,617,914 - RR42_RS32230 0.52 -0.1 2,617,914 - RR42_RS32230 0.52 -0.0 2,617,915 + RR42_RS32230 0.52 -0.5 2,617,915 + RR42_RS32230 0.52 -0.5 2,617,915 + RR42_RS32230 0.52 +0.4 2,617,916 - RR42_RS32230 0.52 -3.5 2,617,916 - RR42_RS32230 0.52 -2.9 2,617,943 + RR42_RS32230 0.55 -0.3 2,617,977 + RR42_RS32230 0.58 +0.3 2,618,058 - RR42_RS32230 0.66 -0.5 2,618,058 - RR42_RS32230 0.66 -3.0 2,618,112 + RR42_RS32230 0.72 -1.8 2,618,413 + -0.0 2,618,425 - -0.6 2,618,470 - -0.6 2,618,470 - -2.1 2,618,555 + RR42_RS32235 0.11 -0.8 2,618,784 + RR42_RS32235 0.30 -1.8 2,618,785 - RR42_RS32235 0.30 -0.7 2,618,872 - RR42_RS32235 0.38 -2.1 2,619,030 + RR42_RS32235 0.51 -1.0 2,619,186 + RR42_RS32235 0.65 -1.1 2,619,187 - RR42_RS32235 0.65 -1.6 2,619,187 - RR42_RS32235 0.65 +1.3 2,619,246 + RR42_RS32235 0.70 -2.3 2,619,246 + RR42_RS32235 0.70 +0.2 2,619,265 - RR42_RS32235 0.72 -1.5 2,619,312 + RR42_RS32235 0.76 -1.1 2,619,313 - RR42_RS32235 0.76 +0.3 2,619,357 - RR42_RS32235 0.79 -0.5 2,619,438 - RR42_RS32235 0.86 -0.3 2,619,462 - RR42_RS32235 0.88 -1.7 2,619,492 + -0.8 2,619,493 - -2.6 2,619,493 - -0.8 2,619,759 - -1.0 2,619,872 + -2.0 2,619,873 - -1.6 2,619,873 - -1.0 2,619,873 - -1.6 2,619,909 + RR42_RS32240 0.12 -3.1 2,619,930 - RR42_RS32240 0.15 -0.8 2,619,930 - RR42_RS32240 0.15 -1.3 2,619,930 - RR42_RS32240 0.15 -2.5 2,619,930 - RR42_RS32240 0.15 -1.3 2,619,968 + RR42_RS32240 0.21 -1.3 2,619,968 + RR42_RS32240 0.21 -2.3 2,619,968 + RR42_RS32240 0.21 -0.6 2,619,968 + RR42_RS32240 0.21 +0.3 2,619,969 - RR42_RS32240 0.21 -1.5 2,619,969 - RR42_RS32240 0.21 -3.1 2,620,092 - RR42_RS32240 0.40 -1.0 2,620,092 - RR42_RS32240 0.40 -1.7 2,620,140 - RR42_RS32240 0.48 -2.6 2,620,140 - RR42_RS32240 0.48 -3.5 2,620,140 - RR42_RS32240 0.48 -1.4 2,620,140 - RR42_RS32240 0.48 -1.9 2,620,140 - RR42_RS32240 0.48 -2.9 2,620,172 + RR42_RS32240 0.52 -3.2 2,620,173 - RR42_RS32240 0.53 -2.6
Or see this region's nucleotide sequence