Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31680 and RR42_RS31685 are separated by 157 nucleotides RR42_RS31685 and RR42_RS31690 are separated by 226 nucleotides RR42_RS31690 and RR42_RS31695 are separated by 63 nucleotides
RR42_RS31680: RR42_RS31680 - hypothetical protein, at 2,487,413 to 2,488,309
_RS31680
RR42_RS31685: RR42_RS31685 - Asp/Glu/hydantoin racemase, at 2,488,467 to 2,489,087
_RS31685
RR42_RS31690: RR42_RS31690 - 4-oxalocrotonate tautomerase, at 2,489,314 to 2,489,727
_RS31690
RR42_RS31695: RR42_RS31695 - hypothetical protein, at 2,489,791 to 2,490,201
_RS31695
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2487.639 kb on + strand, within RR42_RS31680 at 2487.640 kb on - strand, within RR42_RS31680 at 2487.711 kb on + strand, within RR42_RS31680 at 2487.911 kb on + strand, within RR42_RS31680 at 2487.966 kb on + strand, within RR42_RS31680 at 2488.192 kb on - strand, within RR42_RS31680 at 2488.241 kb on + strand at 2488.242 kb on - strand at 2488.245 kb on + strand at 2488.245 kb on + strand at 2488.246 kb on - strand at 2488.324 kb on + strand at 2488.325 kb on - strand at 2488.520 kb on - strand at 2488.611 kb on + strand, within RR42_RS31685 at 2488.612 kb on - strand, within RR42_RS31685 at 2488.839 kb on + strand, within RR42_RS31685 at 2488.839 kb on + strand, within RR42_RS31685 at 2488.839 kb on + strand, within RR42_RS31685 at 2488.951 kb on + strand, within RR42_RS31685 at 2488.952 kb on - strand, within RR42_RS31685 at 2488.979 kb on + strand, within RR42_RS31685 at 2489.069 kb on - strand at 2489.069 kb on - strand at 2489.069 kb on - strand at 2489.087 kb on + strand at 2489.088 kb on - strand at 2489.094 kb on - strand at 2489.283 kb on + strand at 2489.315 kb on + strand at 2489.315 kb on + strand at 2489.316 kb on - strand at 2489.399 kb on + strand, within RR42_RS31690 at 2489.400 kb on - strand, within RR42_RS31690 at 2489.400 kb on - strand, within RR42_RS31690 at 2489.415 kb on + strand, within RR42_RS31690 at 2489.518 kb on + strand, within RR42_RS31690 at 2489.519 kb on - strand, within RR42_RS31690 at 2489.697 kb on + strand at 2489.797 kb on - strand at 2489.797 kb on - strand at 2489.976 kb on - strand, within RR42_RS31695 at 2490.024 kb on - strand, within RR42_RS31695 at 2490.024 kb on - strand, within RR42_RS31695
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,487,639 + RR42_RS31680 0.25 +0.4 2,487,640 - RR42_RS31680 0.25 +1.1 2,487,711 + RR42_RS31680 0.33 -0.1 2,487,911 + RR42_RS31680 0.56 +0.2 2,487,966 + RR42_RS31680 0.62 -2.3 2,488,192 - RR42_RS31680 0.87 +0.7 2,488,241 + -0.6 2,488,242 - -1.9 2,488,245 + +0.8 2,488,245 + +0.5 2,488,246 - -1.2 2,488,324 + +1.3 2,488,325 - +0.3 2,488,520 - -0.4 2,488,611 + RR42_RS31685 0.23 +0.3 2,488,612 - RR42_RS31685 0.23 +1.3 2,488,839 + RR42_RS31685 0.60 -0.4 2,488,839 + RR42_RS31685 0.60 +2.9 2,488,839 + RR42_RS31685 0.60 -0.7 2,488,951 + RR42_RS31685 0.78 -1.6 2,488,952 - RR42_RS31685 0.78 +0.2 2,488,979 + RR42_RS31685 0.82 -2.3 2,489,069 - -0.2 2,489,069 - -2.3 2,489,069 - +0.2 2,489,087 + +0.0 2,489,088 - +1.8 2,489,094 - +1.2 2,489,283 + +0.0 2,489,315 + +0.7 2,489,315 + +2.4 2,489,316 - +0.7 2,489,399 + RR42_RS31690 0.21 +0.9 2,489,400 - RR42_RS31690 0.21 +1.8 2,489,400 - RR42_RS31690 0.21 +0.6 2,489,415 + RR42_RS31690 0.24 +1.8 2,489,518 + RR42_RS31690 0.49 +0.4 2,489,519 - RR42_RS31690 0.50 -0.7 2,489,697 + -0.2 2,489,797 - +0.2 2,489,797 - -0.6 2,489,976 - RR42_RS31695 0.45 +0.6 2,490,024 - RR42_RS31695 0.57 -1.0 2,490,024 - RR42_RS31695 0.57 -0.4
Or see this region's nucleotide sequence