Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31660 and RR42_RS31665 are separated by 55 nucleotides RR42_RS31665 and RR42_RS31670 are separated by 132 nucleotides RR42_RS31670 and RR42_RS31675 are separated by 165 nucleotides RR42_RS31675 and RR42_RS31680 are separated by 28 nucleotides
RR42_RS31660: RR42_RS31660 - hypothetical protein, at 2,483,334 to 2,484,710
_RS31660
RR42_RS31665: RR42_RS31665 - hypothetical protein, at 2,484,766 to 2,485,533
_RS31665
RR42_RS31670: RR42_RS31670 - NAD-dependent dehydratase, at 2,485,666 to 2,486,430
_RS31670
RR42_RS31675: RR42_RS31675 - enoyl-CoA hydratase, at 2,486,596 to 2,487,384
_RS31675
RR42_RS31680: RR42_RS31680 - hypothetical protein, at 2,487,413 to 2,488,309
_RS31680
Position (kb)
2485
2486
2487 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2484.771 kb on + strand at 2484.812 kb on - strand at 2484.815 kb on + strand at 2485.022 kb on - strand, within RR42_RS31665 at 2485.022 kb on - strand, within RR42_RS31665 at 2485.055 kb on + strand, within RR42_RS31665 at 2485.055 kb on + strand, within RR42_RS31665 at 2485.092 kb on + strand, within RR42_RS31665 at 2485.201 kb on + strand, within RR42_RS31665 at 2485.375 kb on + strand, within RR42_RS31665 at 2485.492 kb on + strand at 2485.509 kb on + strand at 2485.678 kb on + strand at 2485.732 kb on - strand at 2485.857 kb on + strand, within RR42_RS31670 at 2485.857 kb on + strand, within RR42_RS31670 at 2485.945 kb on - strand, within RR42_RS31670 at 2485.947 kb on + strand, within RR42_RS31670 at 2485.948 kb on - strand, within RR42_RS31670 at 2485.948 kb on - strand, within RR42_RS31670 at 2485.962 kb on + strand, within RR42_RS31670 at 2485.962 kb on + strand, within RR42_RS31670 at 2485.962 kb on + strand, within RR42_RS31670 at 2486.083 kb on + strand, within RR42_RS31670 at 2486.089 kb on + strand, within RR42_RS31670 at 2486.090 kb on - strand, within RR42_RS31670 at 2486.161 kb on + strand, within RR42_RS31670 at 2486.161 kb on + strand, within RR42_RS31670 at 2486.263 kb on + strand, within RR42_RS31670 at 2486.301 kb on + strand, within RR42_RS31670 at 2486.302 kb on - strand, within RR42_RS31670 at 2486.305 kb on + strand, within RR42_RS31670 at 2486.306 kb on - strand, within RR42_RS31670 at 2486.306 kb on - strand, within RR42_RS31670 at 2486.308 kb on + strand, within RR42_RS31670 at 2486.309 kb on - strand, within RR42_RS31670 at 2486.961 kb on - strand, within RR42_RS31675 at 2486.961 kb on - strand, within RR42_RS31675 at 2487.143 kb on + strand, within RR42_RS31675 at 2487.143 kb on + strand, within RR42_RS31675 at 2487.219 kb on + strand, within RR42_RS31675 at 2487.219 kb on + strand, within RR42_RS31675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,484,771 + -0.7 2,484,812 - +1.3 2,484,815 + -0.0 2,485,022 - RR42_RS31665 0.33 -0.8 2,485,022 - RR42_RS31665 0.33 -1.6 2,485,055 + RR42_RS31665 0.38 -2.1 2,485,055 + RR42_RS31665 0.38 +0.5 2,485,092 + RR42_RS31665 0.42 -0.0 2,485,201 + RR42_RS31665 0.57 -0.5 2,485,375 + RR42_RS31665 0.79 +1.3 2,485,492 + -1.6 2,485,509 + -1.0 2,485,678 + -0.4 2,485,732 - +0.5 2,485,857 + RR42_RS31670 0.25 +0.8 2,485,857 + RR42_RS31670 0.25 +0.2 2,485,945 - RR42_RS31670 0.36 -0.1 2,485,947 + RR42_RS31670 0.37 -1.2 2,485,948 - RR42_RS31670 0.37 -0.7 2,485,948 - RR42_RS31670 0.37 -0.2 2,485,962 + RR42_RS31670 0.39 +0.2 2,485,962 + RR42_RS31670 0.39 -1.2 2,485,962 + RR42_RS31670 0.39 +2.3 2,486,083 + RR42_RS31670 0.55 +1.9 2,486,089 + RR42_RS31670 0.55 -0.3 2,486,090 - RR42_RS31670 0.55 +0.1 2,486,161 + RR42_RS31670 0.65 -0.6 2,486,161 + RR42_RS31670 0.65 -1.4 2,486,263 + RR42_RS31670 0.78 +0.5 2,486,301 + RR42_RS31670 0.83 -1.4 2,486,302 - RR42_RS31670 0.83 +0.0 2,486,305 + RR42_RS31670 0.84 +0.4 2,486,306 - RR42_RS31670 0.84 -0.4 2,486,306 - RR42_RS31670 0.84 -1.4 2,486,308 + RR42_RS31670 0.84 -1.0 2,486,309 - RR42_RS31670 0.84 -0.7 2,486,961 - RR42_RS31675 0.46 +0.5 2,486,961 - RR42_RS31675 0.46 +0.4 2,487,143 + RR42_RS31675 0.69 +0.4 2,487,143 + RR42_RS31675 0.69 -1.7 2,487,219 + RR42_RS31675 0.79 +0.2 2,487,219 + RR42_RS31675 0.79 -1.3
Or see this region's nucleotide sequence