Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31115 and RR42_RS31120 are separated by 93 nucleotides RR42_RS31120 and RR42_RS31125 are separated by 414 nucleotides RR42_RS31125 and RR42_RS31130 overlap by 22 nucleotides
RR42_RS31115: RR42_RS31115 - ABC transporter substrate-binding protein, at 2,360,587 to 2,361,564
_RS31115
RR42_RS31120: RR42_RS31120 - cupin, at 2,361,658 to 2,362,056
_RS31120
RR42_RS31125: RR42_RS31125 - hypothetical protein, at 2,362,471 to 2,362,740
_RS31125
RR42_RS31130: RR42_RS31130 - hypothetical protein, at 2,362,719 to 2,364,290
_RS31130
Position (kb)
2362
2363 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2361.482 kb on + strand at 2361.575 kb on + strand at 2361.707 kb on + strand, within RR42_RS31120 at 2361.707 kb on + strand, within RR42_RS31120 at 2361.707 kb on + strand, within RR42_RS31120 at 2361.708 kb on - strand, within RR42_RS31120 at 2361.708 kb on - strand, within RR42_RS31120 at 2361.848 kb on + strand, within RR42_RS31120 at 2361.848 kb on + strand, within RR42_RS31120 at 2361.848 kb on + strand, within RR42_RS31120 at 2361.848 kb on + strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2362.028 kb on + strand at 2362.028 kb on + strand at 2362.029 kb on - strand at 2362.029 kb on - strand at 2362.029 kb on - strand at 2362.029 kb on - strand at 2362.180 kb on - strand at 2362.210 kb on + strand at 2362.210 kb on + strand at 2362.211 kb on - strand at 2362.211 kb on - strand at 2362.222 kb on + strand at 2362.284 kb on + strand at 2362.285 kb on - strand at 2362.285 kb on - strand at 2362.285 kb on - strand at 2362.332 kb on + strand at 2362.332 kb on + strand at 2362.333 kb on - strand at 2362.333 kb on - strand at 2362.351 kb on + strand at 2362.351 kb on + strand at 2362.351 kb on + strand at 2362.352 kb on - strand at 2362.603 kb on + strand, within RR42_RS31125 at 2362.603 kb on + strand, within RR42_RS31125 at 2362.604 kb on - strand, within RR42_RS31125 at 2362.613 kb on - strand, within RR42_RS31125 at 2362.613 kb on - strand, within RR42_RS31125 at 2362.775 kb on + strand at 2362.775 kb on + strand at 2362.775 kb on + strand at 2362.776 kb on - strand at 2362.776 kb on - strand at 2362.938 kb on + strand, within RR42_RS31130 at 2362.939 kb on - strand, within RR42_RS31130 at 2362.939 kb on - strand, within RR42_RS31130 at 2363.142 kb on + strand, within RR42_RS31130 at 2363.142 kb on + strand, within RR42_RS31130 at 2363.179 kb on + strand, within RR42_RS31130 at 2363.400 kb on - strand, within RR42_RS31130 at 2363.400 kb on - strand, within RR42_RS31130 at 2363.404 kb on - strand, within RR42_RS31130 at 2363.404 kb on - strand, within RR42_RS31130 at 2363.406 kb on + strand, within RR42_RS31130 at 2363.406 kb on + strand, within RR42_RS31130 at 2363.698 kb on - strand, within RR42_RS31130 at 2363.698 kb on - strand, within RR42_RS31130 at 2363.698 kb on - strand, within RR42_RS31130 at 2363.698 kb on - strand, within RR42_RS31130 at 2363.698 kb on - strand, within RR42_RS31130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,361,482 + -0.3 2,361,575 + -0.5 2,361,707 + RR42_RS31120 0.12 +0.1 2,361,707 + RR42_RS31120 0.12 +0.8 2,361,707 + RR42_RS31120 0.12 -0.5 2,361,708 - RR42_RS31120 0.13 +0.9 2,361,708 - RR42_RS31120 0.13 -1.1 2,361,848 + RR42_RS31120 0.48 +0.8 2,361,848 + RR42_RS31120 0.48 -0.9 2,361,848 + RR42_RS31120 0.48 +0.2 2,361,848 + RR42_RS31120 0.48 -0.1 2,361,849 - RR42_RS31120 0.48 +0.5 2,361,849 - RR42_RS31120 0.48 -0.6 2,361,849 - RR42_RS31120 0.48 -0.3 2,361,849 - RR42_RS31120 0.48 -0.2 2,361,849 - RR42_RS31120 0.48 -0.1 2,361,849 - RR42_RS31120 0.48 +0.3 2,361,849 - RR42_RS31120 0.48 -1.1 2,361,849 - RR42_RS31120 0.48 -1.0 2,361,849 - RR42_RS31120 0.48 +0.4 2,362,028 + +0.7 2,362,028 + -1.7 2,362,029 - -1.1 2,362,029 - -0.5 2,362,029 - +0.3 2,362,029 - +0.5 2,362,180 - +1.0 2,362,210 + -0.3 2,362,210 + +1.3 2,362,211 - +0.5 2,362,211 - -0.2 2,362,222 + +0.2 2,362,284 + +0.2 2,362,285 - -1.4 2,362,285 - -1.9 2,362,285 - +1.1 2,362,332 + -0.2 2,362,332 + +1.3 2,362,333 - -0.2 2,362,333 - +0.1 2,362,351 + +0.3 2,362,351 + +0.0 2,362,351 + +2.3 2,362,352 - +1.7 2,362,603 + RR42_RS31125 0.49 -0.6 2,362,603 + RR42_RS31125 0.49 +0.8 2,362,604 - RR42_RS31125 0.49 -1.5 2,362,613 - RR42_RS31125 0.53 -0.7 2,362,613 - RR42_RS31125 0.53 -1.1 2,362,775 + +0.0 2,362,775 + -0.4 2,362,775 + -1.1 2,362,776 - -1.6 2,362,776 - +0.9 2,362,938 + RR42_RS31130 0.14 +0.2 2,362,939 - RR42_RS31130 0.14 +0.7 2,362,939 - RR42_RS31130 0.14 +0.5 2,363,142 + RR42_RS31130 0.27 -0.6 2,363,142 + RR42_RS31130 0.27 -0.1 2,363,179 + RR42_RS31130 0.29 -0.4 2,363,400 - RR42_RS31130 0.43 -0.6 2,363,400 - RR42_RS31130 0.43 +0.4 2,363,404 - RR42_RS31130 0.44 +0.7 2,363,404 - RR42_RS31130 0.44 +0.6 2,363,406 + RR42_RS31130 0.44 -0.2 2,363,406 + RR42_RS31130 0.44 -1.2 2,363,698 - RR42_RS31130 0.62 +0.9 2,363,698 - RR42_RS31130 0.62 -2.0 2,363,698 - RR42_RS31130 0.62 -0.6 2,363,698 - RR42_RS31130 0.62 -0.5 2,363,698 - RR42_RS31130 0.62 +0.9
Or see this region's nucleotide sequence