Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31100 and RR42_RS31105 are separated by 137 nucleotides RR42_RS31105 and RR42_RS31110 are separated by 7 nucleotides
RR42_RS31100: RR42_RS31100 - oxidoreductase, at 2,357,332 to 2,358,192
_RS31100
RR42_RS31105: RR42_RS31105 - acyl-CoA dehydrogenase, at 2,358,330 to 2,359,502
_RS31105
RR42_RS31110: RR42_RS31110 - ABC transporter substrate-binding protein, at 2,359,510 to 2,360,484
_RS31110
Position (kb)
2358
2359
2360 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 2357.368 kb on + strand at 2357.368 kb on + strand at 2357.369 kb on - strand at 2357.416 kb on + strand at 2357.416 kb on + strand at 2357.416 kb on + strand at 2357.417 kb on - strand at 2357.647 kb on + strand, within RR42_RS31100 at 2357.648 kb on - strand, within RR42_RS31100 at 2357.648 kb on - strand, within RR42_RS31100 at 2357.760 kb on - strand, within RR42_RS31100 at 2357.933 kb on + strand, within RR42_RS31100 at 2357.933 kb on + strand, within RR42_RS31100 at 2358.124 kb on + strand at 2358.163 kb on - strand at 2358.225 kb on - strand at 2358.335 kb on - strand at 2358.335 kb on - strand at 2358.341 kb on + strand at 2358.342 kb on - strand at 2358.415 kb on + strand at 2358.415 kb on + strand at 2358.415 kb on + strand at 2358.416 kb on - strand at 2358.416 kb on - strand at 2358.416 kb on - strand at 2358.416 kb on - strand at 2358.430 kb on + strand at 2358.430 kb on + strand at 2358.430 kb on + strand at 2358.430 kb on + strand at 2358.446 kb on - strand at 2358.458 kb on - strand, within RR42_RS31105 at 2358.458 kb on - strand, within RR42_RS31105 at 2358.523 kb on + strand, within RR42_RS31105 at 2358.523 kb on + strand, within RR42_RS31105 at 2358.533 kb on + strand, within RR42_RS31105 at 2358.569 kb on - strand, within RR42_RS31105 at 2358.596 kb on + strand, within RR42_RS31105 at 2358.597 kb on - strand, within RR42_RS31105 at 2358.597 kb on - strand, within RR42_RS31105 at 2358.695 kb on - strand, within RR42_RS31105 at 2358.784 kb on + strand, within RR42_RS31105 at 2358.784 kb on + strand, within RR42_RS31105 at 2358.784 kb on + strand, within RR42_RS31105 at 2358.784 kb on + strand, within RR42_RS31105 at 2358.785 kb on - strand, within RR42_RS31105 at 2358.846 kb on - strand, within RR42_RS31105 at 2358.928 kb on + strand, within RR42_RS31105 at 2358.929 kb on - strand, within RR42_RS31105 at 2358.929 kb on - strand, within RR42_RS31105 at 2358.929 kb on - strand, within RR42_RS31105 at 2359.232 kb on + strand, within RR42_RS31105 at 2359.232 kb on + strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.233 kb on - strand, within RR42_RS31105 at 2359.327 kb on + strand, within RR42_RS31105 at 2359.328 kb on - strand, within RR42_RS31105 at 2359.328 kb on - strand, within RR42_RS31105 at 2359.424 kb on - strand at 2359.446 kb on + strand at 2359.446 kb on + strand at 2359.446 kb on + strand at 2359.446 kb on + strand at 2359.447 kb on - strand at 2359.506 kb on + strand at 2359.506 kb on + strand at 2359.506 kb on + strand at 2359.741 kb on - strand, within RR42_RS31110 at 2359.741 kb on - strand, within RR42_RS31110 at 2359.934 kb on + strand, within RR42_RS31110 at 2359.934 kb on + strand, within RR42_RS31110 at 2359.934 kb on + strand, within RR42_RS31110 at 2359.934 kb on + strand, within RR42_RS31110 at 2359.935 kb on - strand, within RR42_RS31110 at 2359.935 kb on - strand, within RR42_RS31110 at 2360.150 kb on + strand, within RR42_RS31110 at 2360.150 kb on + strand, within RR42_RS31110 at 2360.150 kb on + strand, within RR42_RS31110 at 2360.399 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,357,368 + +0.3 2,357,368 + +1.3 2,357,369 - -1.0 2,357,416 + -0.5 2,357,416 + +2.2 2,357,416 + -1.1 2,357,417 - +0.0 2,357,647 + RR42_RS31100 0.37 +1.0 2,357,648 - RR42_RS31100 0.37 +0.0 2,357,648 - RR42_RS31100 0.37 -0.2 2,357,760 - RR42_RS31100 0.50 +0.9 2,357,933 + RR42_RS31100 0.70 -0.7 2,357,933 + RR42_RS31100 0.70 +0.0 2,358,124 + -0.2 2,358,163 - +2.3 2,358,225 - -0.3 2,358,335 - -1.7 2,358,335 - +0.3 2,358,341 + +1.3 2,358,342 - -0.1 2,358,415 + +0.1 2,358,415 + -1.0 2,358,415 + +0.5 2,358,416 - -0.7 2,358,416 - -0.4 2,358,416 - -1.2 2,358,416 - -1.4 2,358,430 + +0.1 2,358,430 + +0.0 2,358,430 + +0.1 2,358,430 + -1.1 2,358,446 - -1.1 2,358,458 - RR42_RS31105 0.11 +0.4 2,358,458 - RR42_RS31105 0.11 -0.5 2,358,523 + RR42_RS31105 0.16 -0.3 2,358,523 + RR42_RS31105 0.16 +0.1 2,358,533 + RR42_RS31105 0.17 +0.1 2,358,569 - RR42_RS31105 0.20 -0.0 2,358,596 + RR42_RS31105 0.23 +0.3 2,358,597 - RR42_RS31105 0.23 +7.1 2,358,597 - RR42_RS31105 0.23 +0.4 2,358,695 - RR42_RS31105 0.31 -0.7 2,358,784 + RR42_RS31105 0.39 -0.8 2,358,784 + RR42_RS31105 0.39 +0.0 2,358,784 + RR42_RS31105 0.39 +3.1 2,358,784 + RR42_RS31105 0.39 +0.5 2,358,785 - RR42_RS31105 0.39 -2.2 2,358,846 - RR42_RS31105 0.44 -0.6 2,358,928 + RR42_RS31105 0.51 -0.7 2,358,929 - RR42_RS31105 0.51 -1.4 2,358,929 - RR42_RS31105 0.51 +0.1 2,358,929 - RR42_RS31105 0.51 +0.2 2,359,232 + RR42_RS31105 0.77 -0.3 2,359,232 + RR42_RS31105 0.77 -0.6 2,359,233 - RR42_RS31105 0.77 +1.0 2,359,233 - RR42_RS31105 0.77 -0.2 2,359,233 - RR42_RS31105 0.77 -1.1 2,359,233 - RR42_RS31105 0.77 -0.7 2,359,233 - RR42_RS31105 0.77 +0.5 2,359,233 - RR42_RS31105 0.77 +0.1 2,359,233 - RR42_RS31105 0.77 +1.2 2,359,327 + RR42_RS31105 0.85 -1.4 2,359,328 - RR42_RS31105 0.85 -2.1 2,359,328 - RR42_RS31105 0.85 -0.7 2,359,424 - -0.3 2,359,446 + +1.4 2,359,446 + -0.7 2,359,446 + +0.6 2,359,446 + -0.3 2,359,447 - -1.1 2,359,506 + +0.9 2,359,506 + +0.3 2,359,506 + +1.1 2,359,741 - RR42_RS31110 0.24 -0.1 2,359,741 - RR42_RS31110 0.24 +0.6 2,359,934 + RR42_RS31110 0.43 -1.0 2,359,934 + RR42_RS31110 0.43 +0.5 2,359,934 + RR42_RS31110 0.43 +1.3 2,359,934 + RR42_RS31110 0.43 +0.0 2,359,935 - RR42_RS31110 0.44 -0.7 2,359,935 - RR42_RS31110 0.44 +0.5 2,360,150 + RR42_RS31110 0.66 +0.7 2,360,150 + RR42_RS31110 0.66 -1.7 2,360,150 + RR42_RS31110 0.66 +0.6 2,360,399 + +1.6
Or see this region's nucleotide sequence