Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31105

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31100 and RR42_RS31105 are separated by 137 nucleotidesRR42_RS31105 and RR42_RS31110 are separated by 7 nucleotides RR42_RS31100: RR42_RS31100 - oxidoreductase, at 2,357,332 to 2,358,192 _RS31100 RR42_RS31105: RR42_RS31105 - acyl-CoA dehydrogenase, at 2,358,330 to 2,359,502 _RS31105 RR42_RS31110: RR42_RS31110 - ABC transporter substrate-binding protein, at 2,359,510 to 2,360,484 _RS31110 Position (kb) 2358 2359 2360Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7at 2357.368 kb on + strandat 2357.368 kb on + strandat 2357.369 kb on - strandat 2357.416 kb on + strandat 2357.416 kb on + strandat 2357.416 kb on + strandat 2357.417 kb on - strandat 2357.647 kb on + strand, within RR42_RS31100at 2357.648 kb on - strand, within RR42_RS31100at 2357.648 kb on - strand, within RR42_RS31100at 2357.760 kb on - strand, within RR42_RS31100at 2357.933 kb on + strand, within RR42_RS31100at 2357.933 kb on + strand, within RR42_RS31100at 2358.124 kb on + strandat 2358.163 kb on - strandat 2358.225 kb on - strandat 2358.335 kb on - strandat 2358.335 kb on - strandat 2358.341 kb on + strandat 2358.342 kb on - strandat 2358.415 kb on + strandat 2358.415 kb on + strandat 2358.415 kb on + strandat 2358.416 kb on - strandat 2358.416 kb on - strandat 2358.416 kb on - strandat 2358.416 kb on - strandat 2358.430 kb on + strandat 2358.430 kb on + strandat 2358.430 kb on + strandat 2358.430 kb on + strandat 2358.446 kb on - strandat 2358.458 kb on - strand, within RR42_RS31105at 2358.458 kb on - strand, within RR42_RS31105at 2358.523 kb on + strand, within RR42_RS31105at 2358.523 kb on + strand, within RR42_RS31105at 2358.533 kb on + strand, within RR42_RS31105at 2358.569 kb on - strand, within RR42_RS31105at 2358.596 kb on + strand, within RR42_RS31105at 2358.597 kb on - strand, within RR42_RS31105at 2358.597 kb on - strand, within RR42_RS31105at 2358.695 kb on - strand, within RR42_RS31105at 2358.784 kb on + strand, within RR42_RS31105at 2358.784 kb on + strand, within RR42_RS31105at 2358.784 kb on + strand, within RR42_RS31105at 2358.784 kb on + strand, within RR42_RS31105at 2358.785 kb on - strand, within RR42_RS31105at 2358.846 kb on - strand, within RR42_RS31105at 2358.928 kb on + strand, within RR42_RS31105at 2358.929 kb on - strand, within RR42_RS31105at 2358.929 kb on - strand, within RR42_RS31105at 2358.929 kb on - strand, within RR42_RS31105at 2359.232 kb on + strand, within RR42_RS31105at 2359.232 kb on + strand, within RR42_RS31105at 2359.233 kb on - strand, within RR42_RS31105at 2359.233 kb on - strand, within RR42_RS31105at 2359.233 kb on - strand, within RR42_RS31105at 2359.233 kb on - strand, within RR42_RS31105at 2359.233 kb on - strand, within RR42_RS31105at 2359.233 kb on - strand, within RR42_RS31105at 2359.233 kb on - strand, within RR42_RS31105at 2359.327 kb on + strand, within RR42_RS31105at 2359.328 kb on - strand, within RR42_RS31105at 2359.328 kb on - strand, within RR42_RS31105at 2359.424 kb on - strandat 2359.446 kb on + strandat 2359.446 kb on + strandat 2359.446 kb on + strandat 2359.446 kb on + strandat 2359.447 kb on - strandat 2359.506 kb on + strandat 2359.506 kb on + strandat 2359.506 kb on + strandat 2359.741 kb on - strand, within RR42_RS31110at 2359.741 kb on - strand, within RR42_RS31110at 2359.934 kb on + strand, within RR42_RS31110at 2359.934 kb on + strand, within RR42_RS31110at 2359.934 kb on + strand, within RR42_RS31110at 2359.934 kb on + strand, within RR42_RS31110at 2359.935 kb on - strand, within RR42_RS31110at 2359.935 kb on - strand, within RR42_RS31110at 2360.150 kb on + strand, within RR42_RS31110at 2360.150 kb on + strand, within RR42_RS31110at 2360.150 kb on + strand, within RR42_RS31110at 2360.399 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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2,357,368 + +0.3
2,357,368 + +1.3
2,357,369 - -1.0
2,357,416 + -0.5
2,357,416 + +2.2
2,357,416 + -1.1
2,357,417 - +0.0
2,357,647 + RR42_RS31100 0.37 +1.0
2,357,648 - RR42_RS31100 0.37 +0.0
2,357,648 - RR42_RS31100 0.37 -0.2
2,357,760 - RR42_RS31100 0.50 +0.9
2,357,933 + RR42_RS31100 0.70 -0.7
2,357,933 + RR42_RS31100 0.70 +0.0
2,358,124 + -0.2
2,358,163 - +2.3
2,358,225 - -0.3
2,358,335 - -1.7
2,358,335 - +0.3
2,358,341 + +1.3
2,358,342 - -0.1
2,358,415 + +0.1
2,358,415 + -1.0
2,358,415 + +0.5
2,358,416 - -0.7
2,358,416 - -0.4
2,358,416 - -1.2
2,358,416 - -1.4
2,358,430 + +0.1
2,358,430 + +0.0
2,358,430 + +0.1
2,358,430 + -1.1
2,358,446 - -1.1
2,358,458 - RR42_RS31105 0.11 +0.4
2,358,458 - RR42_RS31105 0.11 -0.5
2,358,523 + RR42_RS31105 0.16 -0.3
2,358,523 + RR42_RS31105 0.16 +0.1
2,358,533 + RR42_RS31105 0.17 +0.1
2,358,569 - RR42_RS31105 0.20 -0.0
2,358,596 + RR42_RS31105 0.23 +0.3
2,358,597 - RR42_RS31105 0.23 +7.1
2,358,597 - RR42_RS31105 0.23 +0.4
2,358,695 - RR42_RS31105 0.31 -0.7
2,358,784 + RR42_RS31105 0.39 -0.8
2,358,784 + RR42_RS31105 0.39 +0.0
2,358,784 + RR42_RS31105 0.39 +3.1
2,358,784 + RR42_RS31105 0.39 +0.5
2,358,785 - RR42_RS31105 0.39 -2.2
2,358,846 - RR42_RS31105 0.44 -0.6
2,358,928 + RR42_RS31105 0.51 -0.7
2,358,929 - RR42_RS31105 0.51 -1.4
2,358,929 - RR42_RS31105 0.51 +0.1
2,358,929 - RR42_RS31105 0.51 +0.2
2,359,232 + RR42_RS31105 0.77 -0.3
2,359,232 + RR42_RS31105 0.77 -0.6
2,359,233 - RR42_RS31105 0.77 +1.0
2,359,233 - RR42_RS31105 0.77 -0.2
2,359,233 - RR42_RS31105 0.77 -1.1
2,359,233 - RR42_RS31105 0.77 -0.7
2,359,233 - RR42_RS31105 0.77 +0.5
2,359,233 - RR42_RS31105 0.77 +0.1
2,359,233 - RR42_RS31105 0.77 +1.2
2,359,327 + RR42_RS31105 0.85 -1.4
2,359,328 - RR42_RS31105 0.85 -2.1
2,359,328 - RR42_RS31105 0.85 -0.7
2,359,424 - -0.3
2,359,446 + +1.4
2,359,446 + -0.7
2,359,446 + +0.6
2,359,446 + -0.3
2,359,447 - -1.1
2,359,506 + +0.9
2,359,506 + +0.3
2,359,506 + +1.1
2,359,741 - RR42_RS31110 0.24 -0.1
2,359,741 - RR42_RS31110 0.24 +0.6
2,359,934 + RR42_RS31110 0.43 -1.0
2,359,934 + RR42_RS31110 0.43 +0.5
2,359,934 + RR42_RS31110 0.43 +1.3
2,359,934 + RR42_RS31110 0.43 +0.0
2,359,935 - RR42_RS31110 0.44 -0.7
2,359,935 - RR42_RS31110 0.44 +0.5
2,360,150 + RR42_RS31110 0.66 +0.7
2,360,150 + RR42_RS31110 0.66 -1.7
2,360,150 + RR42_RS31110 0.66 +0.6
2,360,399 + +1.6

Or see this region's nucleotide sequence