Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30210 and RR42_RS30215 are separated by 478 nucleotides RR42_RS30215 and RR42_RS30220 are separated by 144 nucleotides
RR42_RS30210: RR42_RS30210 - LysR family transcriptional regulator, at 2,152,767 to 2,153,651
_RS30210
RR42_RS30215: RR42_RS30215 - citrate:proton symporter, at 2,154,130 to 2,155,428
_RS30215
RR42_RS30220: RR42_RS30220 - porin, at 2,155,573 to 2,156,727
_RS30220
Position (kb)
2154
2155
2156 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2153.254 kb on - strand, within RR42_RS30210 at 2153.254 kb on - strand, within RR42_RS30210 at 2153.314 kb on + strand, within RR42_RS30210 at 2153.314 kb on + strand, within RR42_RS30210 at 2153.354 kb on + strand, within RR42_RS30210 at 2153.354 kb on + strand, within RR42_RS30210 at 2153.476 kb on + strand, within RR42_RS30210 at 2153.729 kb on + strand at 2153.730 kb on - strand at 2153.893 kb on + strand at 2153.893 kb on + strand at 2153.893 kb on + strand at 2153.897 kb on + strand at 2153.897 kb on + strand at 2153.897 kb on + strand at 2153.897 kb on + strand at 2153.898 kb on - strand at 2153.898 kb on - strand at 2153.900 kb on + strand at 2153.935 kb on + strand at 2153.936 kb on - strand at 2153.936 kb on - strand at 2154.070 kb on - strand at 2154.092 kb on + strand at 2154.093 kb on - strand at 2154.132 kb on - strand at 2154.217 kb on + strand at 2154.217 kb on + strand at 2154.232 kb on + strand at 2154.232 kb on + strand at 2154.250 kb on + strand at 2154.328 kb on + strand, within RR42_RS30215 at 2154.328 kb on + strand, within RR42_RS30215 at 2154.329 kb on - strand, within RR42_RS30215 at 2154.357 kb on + strand, within RR42_RS30215 at 2154.367 kb on + strand, within RR42_RS30215 at 2154.368 kb on - strand, within RR42_RS30215 at 2154.495 kb on - strand, within RR42_RS30215 at 2154.622 kb on - strand, within RR42_RS30215 at 2154.630 kb on + strand, within RR42_RS30215 at 2154.631 kb on - strand, within RR42_RS30215 at 2154.664 kb on - strand, within RR42_RS30215 at 2154.670 kb on + strand, within RR42_RS30215 at 2154.671 kb on - strand, within RR42_RS30215 at 2154.671 kb on - strand, within RR42_RS30215 at 2154.671 kb on - strand, within RR42_RS30215 at 2154.671 kb on - strand, within RR42_RS30215 at 2154.671 kb on - strand, within RR42_RS30215 at 2154.671 kb on - strand, within RR42_RS30215 at 2154.672 kb on + strand, within RR42_RS30215 at 2154.672 kb on + strand, within RR42_RS30215 at 2154.672 kb on + strand, within RR42_RS30215 at 2154.673 kb on - strand, within RR42_RS30215 at 2154.940 kb on + strand, within RR42_RS30215 at 2154.940 kb on + strand, within RR42_RS30215 at 2154.940 kb on + strand, within RR42_RS30215 at 2155.227 kb on - strand, within RR42_RS30215 at 2155.267 kb on + strand, within RR42_RS30215 at 2155.267 kb on + strand, within RR42_RS30215 at 2155.267 kb on + strand, within RR42_RS30215 at 2155.269 kb on + strand, within RR42_RS30215 at 2155.327 kb on + strand at 2155.328 kb on - strand at 2155.360 kb on + strand at 2155.361 kb on - strand at 2155.361 kb on - strand at 2155.427 kb on - strand at 2155.435 kb on + strand at 2155.436 kb on - strand at 2155.436 kb on - strand at 2155.436 kb on - strand at 2155.622 kb on - strand at 2155.663 kb on + strand at 2155.719 kb on + strand, within RR42_RS30220 at 2155.720 kb on - strand, within RR42_RS30220 at 2156.080 kb on + strand, within RR42_RS30220 at 2156.080 kb on + strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.081 kb on - strand, within RR42_RS30220 at 2156.182 kb on + strand, within RR42_RS30220 at 2156.183 kb on - strand, within RR42_RS30220 at 2156.288 kb on - strand, within RR42_RS30220 at 2156.289 kb on + strand, within RR42_RS30220 at 2156.311 kb on + strand, within RR42_RS30220 at 2156.395 kb on + strand, within RR42_RS30220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,153,254 - RR42_RS30210 0.55 +0.3 2,153,254 - RR42_RS30210 0.55 -0.3 2,153,314 + RR42_RS30210 0.62 +0.2 2,153,314 + RR42_RS30210 0.62 +0.9 2,153,354 + RR42_RS30210 0.66 +1.3 2,153,354 + RR42_RS30210 0.66 -2.6 2,153,476 + RR42_RS30210 0.80 +1.3 2,153,729 + +1.0 2,153,730 - -0.1 2,153,893 + +1.9 2,153,893 + -0.3 2,153,893 + +0.3 2,153,897 + +0.0 2,153,897 + +0.4 2,153,897 + +1.2 2,153,897 + -0.8 2,153,898 - +0.0 2,153,898 - -0.5 2,153,900 + -0.1 2,153,935 + -1.3 2,153,936 - -1.5 2,153,936 - -1.1 2,154,070 - +0.8 2,154,092 + +0.7 2,154,093 - +0.7 2,154,132 - +0.5 2,154,217 + +0.5 2,154,217 + +0.3 2,154,232 + +0.3 2,154,232 + -0.3 2,154,250 + -0.3 2,154,328 + RR42_RS30215 0.15 -0.1 2,154,328 + RR42_RS30215 0.15 -0.6 2,154,329 - RR42_RS30215 0.15 -0.2 2,154,357 + RR42_RS30215 0.17 -0.6 2,154,367 + RR42_RS30215 0.18 +1.3 2,154,368 - RR42_RS30215 0.18 -0.0 2,154,495 - RR42_RS30215 0.28 +0.6 2,154,622 - RR42_RS30215 0.38 +1.3 2,154,630 + RR42_RS30215 0.38 +0.9 2,154,631 - RR42_RS30215 0.39 -0.5 2,154,664 - RR42_RS30215 0.41 -0.3 2,154,670 + RR42_RS30215 0.42 -0.6 2,154,671 - RR42_RS30215 0.42 +1.4 2,154,671 - RR42_RS30215 0.42 -1.2 2,154,671 - RR42_RS30215 0.42 +0.5 2,154,671 - RR42_RS30215 0.42 +0.3 2,154,671 - RR42_RS30215 0.42 -0.8 2,154,671 - RR42_RS30215 0.42 +0.5 2,154,672 + RR42_RS30215 0.42 +1.3 2,154,672 + RR42_RS30215 0.42 -0.3 2,154,672 + RR42_RS30215 0.42 -0.7 2,154,673 - RR42_RS30215 0.42 -0.5 2,154,940 + RR42_RS30215 0.62 -2.3 2,154,940 + RR42_RS30215 0.62 -1.0 2,154,940 + RR42_RS30215 0.62 +0.5 2,155,227 - RR42_RS30215 0.84 +0.7 2,155,267 + RR42_RS30215 0.88 +0.3 2,155,267 + RR42_RS30215 0.88 -0.8 2,155,267 + RR42_RS30215 0.88 +0.5 2,155,269 + RR42_RS30215 0.88 +0.1 2,155,327 + +0.4 2,155,328 - +0.2 2,155,360 + +0.3 2,155,361 - -1.9 2,155,361 - -0.1 2,155,427 - -0.1 2,155,435 + +0.3 2,155,436 - -0.8 2,155,436 - -0.8 2,155,436 - +0.8 2,155,622 - +0.1 2,155,663 + +0.7 2,155,719 + RR42_RS30220 0.13 +0.7 2,155,720 - RR42_RS30220 0.13 -0.9 2,156,080 + RR42_RS30220 0.44 +0.4 2,156,080 + RR42_RS30220 0.44 +0.7 2,156,081 - RR42_RS30220 0.44 -0.2 2,156,081 - RR42_RS30220 0.44 +0.6 2,156,081 - RR42_RS30220 0.44 +1.3 2,156,081 - RR42_RS30220 0.44 -1.9 2,156,081 - RR42_RS30220 0.44 +0.2 2,156,081 - RR42_RS30220 0.44 -0.8 2,156,081 - RR42_RS30220 0.44 -1.1 2,156,081 - RR42_RS30220 0.44 -0.5 2,156,182 + RR42_RS30220 0.53 -1.9 2,156,183 - RR42_RS30220 0.53 -0.3 2,156,288 - RR42_RS30220 0.62 -0.1 2,156,289 + RR42_RS30220 0.62 -1.5 2,156,311 + RR42_RS30220 0.64 -0.7 2,156,395 + RR42_RS30220 0.71 +0.2
Or see this region's nucleotide sequence