Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30090 and RR42_RS30095 are separated by 183 nucleotides RR42_RS30095 and RR42_RS30100 are separated by 73 nucleotides
RR42_RS30090: RR42_RS30090 - MFS transporter, at 2,122,565 to 2,123,836
_RS30090
RR42_RS30095: RR42_RS30095 - alcohol dehydrogenase, at 2,124,020 to 2,124,991
_RS30095
RR42_RS30100: RR42_RS30100 - transposase, at 2,125,065 to 2,126,123
_RS30100
Position (kb)
2124
2125 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2123.062 kb on - strand, within RR42_RS30090 at 2123.233 kb on - strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.489 kb on + strand, within RR42_RS30090 at 2123.489 kb on + strand, within RR42_RS30090 at 2123.499 kb on + strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.525 kb on - strand, within RR42_RS30090 at 2123.531 kb on + strand, within RR42_RS30090 at 2123.900 kb on + strand at 2123.900 kb on + strand at 2123.901 kb on - strand at 2124.044 kb on + strand at 2124.190 kb on + strand, within RR42_RS30095 at 2124.191 kb on - strand, within RR42_RS30095 at 2124.192 kb on + strand, within RR42_RS30095 at 2124.192 kb on + strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.559 kb on - strand, within RR42_RS30095 at 2124.606 kb on + strand, within RR42_RS30095 at 2124.606 kb on + strand, within RR42_RS30095 at 2124.735 kb on + strand, within RR42_RS30095 at 2124.838 kb on - strand, within RR42_RS30095 at 2124.855 kb on + strand, within RR42_RS30095 at 2124.856 kb on - strand, within RR42_RS30095 at 2124.856 kb on - strand, within RR42_RS30095 at 2124.859 kb on + strand, within RR42_RS30095 at 2124.882 kb on + strand, within RR42_RS30095 at 2124.882 kb on + strand, within RR42_RS30095 at 2124.882 kb on + strand, within RR42_RS30095 at 2124.883 kb on - strand, within RR42_RS30095 at 2124.924 kb on - strand at 2125.007 kb on + strand at 2125.007 kb on + strand at 2125.007 kb on + strand at 2125.008 kb on - strand at 2125.008 kb on - strand at 2125.008 kb on - strand at 2125.426 kb on + strand, within RR42_RS30100 at 2125.489 kb on + strand, within RR42_RS30100 at 2125.489 kb on + strand, within RR42_RS30100 at 2125.489 kb on + strand, within RR42_RS30100 at 2125.490 kb on - strand, within RR42_RS30100 at 2125.541 kb on - strand, within RR42_RS30100 at 2125.633 kb on + strand, within RR42_RS30100 at 2125.633 kb on + strand, within RR42_RS30100 at 2125.633 kb on + strand, within RR42_RS30100 at 2125.685 kb on - strand, within RR42_RS30100 at 2125.972 kb on + strand, within RR42_RS30100 at 2125.972 kb on + strand, within RR42_RS30100 at 2125.972 kb on + strand, within RR42_RS30100 at 2125.973 kb on - strand, within RR42_RS30100
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,123,062 - RR42_RS30090 0.39 -0.4 2,123,233 - RR42_RS30090 0.53 +0.5 2,123,384 + RR42_RS30090 0.64 -0.0 2,123,384 + RR42_RS30090 0.64 -1.3 2,123,384 + RR42_RS30090 0.64 -0.8 2,123,384 + RR42_RS30090 0.64 +1.3 2,123,385 - RR42_RS30090 0.64 -1.5 2,123,385 - RR42_RS30090 0.64 -1.5 2,123,385 - RR42_RS30090 0.64 +0.5 2,123,385 - RR42_RS30090 0.64 +1.0 2,123,489 + RR42_RS30090 0.73 +1.3 2,123,489 + RR42_RS30090 0.73 -0.9 2,123,499 + RR42_RS30090 0.73 +0.2 2,123,500 - RR42_RS30090 0.74 -0.2 2,123,500 - RR42_RS30090 0.74 -1.8 2,123,500 - RR42_RS30090 0.74 -0.7 2,123,525 - RR42_RS30090 0.75 -2.0 2,123,531 + RR42_RS30090 0.76 -0.7 2,123,900 + -1.4 2,123,900 + -1.5 2,123,901 - -0.2 2,124,044 + -0.1 2,124,190 + RR42_RS30095 0.17 -0.0 2,124,191 - RR42_RS30095 0.18 +0.7 2,124,192 + RR42_RS30095 0.18 +0.8 2,124,192 + RR42_RS30095 0.18 -0.3 2,124,193 - RR42_RS30095 0.18 +1.2 2,124,193 - RR42_RS30095 0.18 -0.3 2,124,193 - RR42_RS30095 0.18 +0.0 2,124,193 - RR42_RS30095 0.18 +0.1 2,124,559 - RR42_RS30095 0.55 -0.8 2,124,606 + RR42_RS30095 0.60 +2.3 2,124,606 + RR42_RS30095 0.60 +1.4 2,124,735 + RR42_RS30095 0.74 -0.7 2,124,838 - RR42_RS30095 0.84 -1.0 2,124,855 + RR42_RS30095 0.86 +0.9 2,124,856 - RR42_RS30095 0.86 -0.6 2,124,856 - RR42_RS30095 0.86 -0.7 2,124,859 + RR42_RS30095 0.86 -0.0 2,124,882 + RR42_RS30095 0.89 -0.2 2,124,882 + RR42_RS30095 0.89 -0.1 2,124,882 + RR42_RS30095 0.89 +1.0 2,124,883 - RR42_RS30095 0.89 +0.3 2,124,924 - +0.2 2,125,007 + -0.0 2,125,007 + -0.3 2,125,007 + -1.3 2,125,008 - -0.1 2,125,008 - -2.6 2,125,008 - -0.7 2,125,426 + RR42_RS30100 0.34 -0.1 2,125,489 + RR42_RS30100 0.40 -0.6 2,125,489 + RR42_RS30100 0.40 -0.5 2,125,489 + RR42_RS30100 0.40 -0.9 2,125,490 - RR42_RS30100 0.40 -0.0 2,125,541 - RR42_RS30100 0.45 -0.2 2,125,633 + RR42_RS30100 0.54 -0.5 2,125,633 + RR42_RS30100 0.54 -0.4 2,125,633 + RR42_RS30100 0.54 -1.4 2,125,685 - RR42_RS30100 0.59 +1.6 2,125,972 + RR42_RS30100 0.86 -0.8 2,125,972 + RR42_RS30100 0.86 +0.8 2,125,972 + RR42_RS30100 0.86 +0.1 2,125,973 - RR42_RS30100 0.86 -1.3
Or see this region's nucleotide sequence