Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30080 and RR42_RS30085 are separated by 16 nucleotides RR42_RS30085 and RR42_RS30090 are separated by 475 nucleotides RR42_RS30090 and RR42_RS30095 are separated by 183 nucleotides
RR42_RS30080: RR42_RS30080 - MFS transporter, at 2,120,319 to 2,121,707
_RS30080
RR42_RS30085: RR42_RS30085 - hypothetical protein, at 2,121,724 to 2,122,089
_RS30085
RR42_RS30090: RR42_RS30090 - MFS transporter, at 2,122,565 to 2,123,836
_RS30090
RR42_RS30095: RR42_RS30095 - alcohol dehydrogenase, at 2,124,020 to 2,124,991
_RS30095
Position (kb)
2122
2123
2124 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2121.620 kb on + strand at 2121.651 kb on + strand at 2121.775 kb on + strand, within RR42_RS30085 at 2121.776 kb on - strand, within RR42_RS30085 at 2121.940 kb on + strand, within RR42_RS30085 at 2121.941 kb on - strand, within RR42_RS30085 at 2121.941 kb on - strand, within RR42_RS30085 at 2122.083 kb on + strand at 2122.084 kb on - strand at 2122.084 kb on - strand at 2122.084 kb on - strand at 2122.109 kb on - strand at 2122.119 kb on + strand at 2122.119 kb on + strand at 2122.120 kb on - strand at 2122.123 kb on + strand at 2122.162 kb on + strand at 2122.239 kb on - strand at 2122.239 kb on - strand at 2122.374 kb on - strand at 2122.527 kb on - strand at 2122.535 kb on - strand at 2122.861 kb on + strand, within RR42_RS30090 at 2123.062 kb on - strand, within RR42_RS30090 at 2123.233 kb on - strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.489 kb on + strand, within RR42_RS30090 at 2123.489 kb on + strand, within RR42_RS30090 at 2123.499 kb on + strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.525 kb on - strand, within RR42_RS30090 at 2123.531 kb on + strand, within RR42_RS30090 at 2123.900 kb on + strand at 2123.900 kb on + strand at 2123.901 kb on - strand at 2124.044 kb on + strand at 2124.190 kb on + strand, within RR42_RS30095 at 2124.191 kb on - strand, within RR42_RS30095 at 2124.192 kb on + strand, within RR42_RS30095 at 2124.192 kb on + strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.559 kb on - strand, within RR42_RS30095 at 2124.606 kb on + strand, within RR42_RS30095 at 2124.606 kb on + strand, within RR42_RS30095 at 2124.735 kb on + strand, within RR42_RS30095
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,121,620 + -0.7 2,121,651 + +0.7 2,121,775 + RR42_RS30085 0.14 +0.7 2,121,776 - RR42_RS30085 0.14 -0.3 2,121,940 + RR42_RS30085 0.59 -0.4 2,121,941 - RR42_RS30085 0.59 -0.8 2,121,941 - RR42_RS30085 0.59 -0.3 2,122,083 + -1.4 2,122,084 - -0.1 2,122,084 - +0.1 2,122,084 - +0.5 2,122,109 - +0.5 2,122,119 + -1.7 2,122,119 + -0.6 2,122,120 - +0.4 2,122,123 + -0.3 2,122,162 + -1.1 2,122,239 - -1.2 2,122,239 - -2.1 2,122,374 - +0.9 2,122,527 - +2.3 2,122,535 - -0.5 2,122,861 + RR42_RS30090 0.23 +0.1 2,123,062 - RR42_RS30090 0.39 -0.4 2,123,233 - RR42_RS30090 0.53 +0.5 2,123,384 + RR42_RS30090 0.64 -0.0 2,123,384 + RR42_RS30090 0.64 -1.3 2,123,384 + RR42_RS30090 0.64 -0.8 2,123,384 + RR42_RS30090 0.64 +1.3 2,123,385 - RR42_RS30090 0.64 -1.5 2,123,385 - RR42_RS30090 0.64 -1.5 2,123,385 - RR42_RS30090 0.64 +0.5 2,123,385 - RR42_RS30090 0.64 +1.0 2,123,489 + RR42_RS30090 0.73 +1.3 2,123,489 + RR42_RS30090 0.73 -0.9 2,123,499 + RR42_RS30090 0.73 +0.2 2,123,500 - RR42_RS30090 0.74 -0.2 2,123,500 - RR42_RS30090 0.74 -1.8 2,123,500 - RR42_RS30090 0.74 -0.7 2,123,525 - RR42_RS30090 0.75 -2.0 2,123,531 + RR42_RS30090 0.76 -0.7 2,123,900 + -1.4 2,123,900 + -1.5 2,123,901 - -0.2 2,124,044 + -0.1 2,124,190 + RR42_RS30095 0.17 -0.0 2,124,191 - RR42_RS30095 0.18 +0.7 2,124,192 + RR42_RS30095 0.18 +0.8 2,124,192 + RR42_RS30095 0.18 -0.3 2,124,193 - RR42_RS30095 0.18 +1.2 2,124,193 - RR42_RS30095 0.18 -0.3 2,124,193 - RR42_RS30095 0.18 +0.0 2,124,193 - RR42_RS30095 0.18 +0.1 2,124,559 - RR42_RS30095 0.55 -0.8 2,124,606 + RR42_RS30095 0.60 +2.3 2,124,606 + RR42_RS30095 0.60 +1.4 2,124,735 + RR42_RS30095 0.74 -0.7
Or see this region's nucleotide sequence