Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS29485

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS29480 and RR42_RS29485 are separated by 38 nucleotidesRR42_RS29485 and RR42_RS29490 are separated by 264 nucleotidesRR42_RS29490 and RR42_RS29495 are separated by 2 nucleotides RR42_RS29480: RR42_RS29480 - AraC family transcriptional regulator, at 1,971,159 to 1,971,929 _RS29480 RR42_RS29485: RR42_RS29485 - fatty-acid--CoA ligase, at 1,971,968 to 1,973,593 _RS29485 RR42_RS29490: RR42_RS29490 - toxin HipA, at 1,973,858 to 1,974,109 _RS29490 RR42_RS29495: RR42_RS29495 - toxin HipA, at 1,974,112 to 1,975,452 _RS29495 Position (kb) 1971 1972 1973 1974Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1970.994 kb on + strandat 1970.995 kb on - strandat 1971.053 kb on - strandat 1971.222 kb on + strandat 1971.223 kb on - strandat 1971.276 kb on + strand, within RR42_RS29480at 1971.276 kb on + strand, within RR42_RS29480at 1971.276 kb on + strand, within RR42_RS29480at 1971.276 kb on + strand, within RR42_RS29480at 1971.276 kb on + strand, within RR42_RS29480at 1971.277 kb on - strand, within RR42_RS29480at 1971.296 kb on + strand, within RR42_RS29480at 1971.296 kb on + strand, within RR42_RS29480at 1971.357 kb on + strand, within RR42_RS29480at 1971.357 kb on + strand, within RR42_RS29480at 1971.357 kb on + strand, within RR42_RS29480at 1971.357 kb on + strand, within RR42_RS29480at 1971.357 kb on + strand, within RR42_RS29480at 1971.358 kb on - strand, within RR42_RS29480at 1971.358 kb on - strand, within RR42_RS29480at 1971.451 kb on + strand, within RR42_RS29480at 1971.516 kb on + strand, within RR42_RS29480at 1971.517 kb on - strand, within RR42_RS29480at 1971.517 kb on - strand, within RR42_RS29480at 1971.789 kb on + strand, within RR42_RS29480at 1971.789 kb on + strand, within RR42_RS29480at 1971.790 kb on - strand, within RR42_RS29480at 1971.790 kb on - strand, within RR42_RS29480at 1971.790 kb on - strand, within RR42_RS29480at 1971.790 kb on - strand, within RR42_RS29480at 1971.790 kb on - strand, within RR42_RS29480at 1971.790 kb on - strand, within RR42_RS29480at 1971.900 kb on - strandat 1972.021 kb on + strandat 1972.086 kb on + strandat 1972.086 kb on + strandat 1972.086 kb on + strandat 1972.161 kb on + strand, within RR42_RS29485at 1972.161 kb on + strand, within RR42_RS29485at 1972.161 kb on + strand, within RR42_RS29485at 1972.161 kb on + strand, within RR42_RS29485at 1972.161 kb on + strand, within RR42_RS29485at 1972.162 kb on - strand, within RR42_RS29485at 1972.162 kb on - strand, within RR42_RS29485at 1972.162 kb on - strand, within RR42_RS29485at 1972.162 kb on - strand, within RR42_RS29485at 1972.162 kb on - strand, within RR42_RS29485at 1972.162 kb on - strand, within RR42_RS29485at 1972.162 kb on - strand, within RR42_RS29485at 1972.162 kb on - strand, within RR42_RS29485at 1972.164 kb on + strand, within RR42_RS29485at 1972.165 kb on - strandat 1972.286 kb on - strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.287 kb on + strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.288 kb on - strand, within RR42_RS29485at 1972.291 kb on + strand, within RR42_RS29485at 1972.291 kb on + strand, within RR42_RS29485at 1972.292 kb on - strand, within RR42_RS29485at 1972.292 kb on - strand, within RR42_RS29485at 1972.292 kb on - strand, within RR42_RS29485at 1972.292 kb on - strand, within RR42_RS29485at 1972.292 kb on - strand, within RR42_RS29485at 1972.318 kb on + strand, within RR42_RS29485at 1972.320 kb on + strand, within RR42_RS29485at 1972.320 kb on + strand, within RR42_RS29485at 1972.320 kb on + strand, within RR42_RS29485at 1972.321 kb on - strand, within RR42_RS29485at 1972.321 kb on - strand, within RR42_RS29485at 1972.596 kb on + strand, within RR42_RS29485at 1973.019 kb on + strand, within RR42_RS29485at 1973.020 kb on - strand, within RR42_RS29485at 1973.020 kb on - strand, within RR42_RS29485at 1973.020 kb on - strand, within RR42_RS29485at 1973.296 kb on + strand, within RR42_RS29485at 1973.296 kb on + strand, within RR42_RS29485at 1973.296 kb on + strand, within RR42_RS29485at 1973.296 kb on + strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.297 kb on - strand, within RR42_RS29485at 1973.515 kb on - strandat 1973.664 kb on + strandat 1973.664 kb on + strandat 1973.665 kb on - strandat 1973.672 kb on + strandat 1973.672 kb on + strandat 1973.672 kb on + strandat 1973.673 kb on - strandat 1973.763 kb on - strandat 1973.763 kb on - strandat 1973.805 kb on + strandat 1973.984 kb on - strand, within RR42_RS29490at 1974.068 kb on - strand, within RR42_RS29490at 1974.107 kb on + strandat 1974.108 kb on - strandat 1974.146 kb on + strandat 1974.168 kb on - strandat 1974.212 kb on - strandat 1974.279 kb on + strand, within RR42_RS29495at 1974.279 kb on + strand, within RR42_RS29495at 1974.280 kb on - strand, within RR42_RS29495at 1974.313 kb on + strand, within RR42_RS29495

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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1,970,994 + +0.8
1,970,995 - +0.8
1,971,053 - -0.5
1,971,222 + -0.4
1,971,223 - +1.6
1,971,276 + RR42_RS29480 0.15 +1.3
1,971,276 + RR42_RS29480 0.15 -0.7
1,971,276 + RR42_RS29480 0.15 +0.3
1,971,276 + RR42_RS29480 0.15 +0.1
1,971,276 + RR42_RS29480 0.15 +0.7
1,971,277 - RR42_RS29480 0.15 +0.0
1,971,296 + RR42_RS29480 0.18 -0.2
1,971,296 + RR42_RS29480 0.18 -0.4
1,971,357 + RR42_RS29480 0.26 -0.2
1,971,357 + RR42_RS29480 0.26 +0.9
1,971,357 + RR42_RS29480 0.26 -0.3
1,971,357 + RR42_RS29480 0.26 -0.1
1,971,357 + RR42_RS29480 0.26 +0.3
1,971,358 - RR42_RS29480 0.26 -0.3
1,971,358 - RR42_RS29480 0.26 +0.8
1,971,451 + RR42_RS29480 0.38 -0.1
1,971,516 + RR42_RS29480 0.46 -0.0
1,971,517 - RR42_RS29480 0.46 +1.7
1,971,517 - RR42_RS29480 0.46 +0.7
1,971,789 + RR42_RS29480 0.82 -1.9
1,971,789 + RR42_RS29480 0.82 +0.4
1,971,790 - RR42_RS29480 0.82 +0.8
1,971,790 - RR42_RS29480 0.82 -1.2
1,971,790 - RR42_RS29480 0.82 +0.3
1,971,790 - RR42_RS29480 0.82 +0.2
1,971,790 - RR42_RS29480 0.82 -0.3
1,971,790 - RR42_RS29480 0.82 +1.3
1,971,900 - +0.1
1,972,021 + -0.1
1,972,086 + -0.5
1,972,086 + +0.1
1,972,086 + +0.1
1,972,161 + RR42_RS29485 0.12 -0.4
1,972,161 + RR42_RS29485 0.12 -2.2
1,972,161 + RR42_RS29485 0.12 +0.2
1,972,161 + RR42_RS29485 0.12 -0.6
1,972,161 + RR42_RS29485 0.12 +0.7
1,972,162 - RR42_RS29485 0.12 -2.3
1,972,162 - RR42_RS29485 0.12 +0.3
1,972,162 - RR42_RS29485 0.12 +0.3
1,972,162 - RR42_RS29485 0.12 +0.7
1,972,162 - RR42_RS29485 0.12 -1.2
1,972,162 - RR42_RS29485 0.12 -1.0
1,972,162 - RR42_RS29485 0.12 -0.2
1,972,162 - RR42_RS29485 0.12 -0.1
1,972,164 + RR42_RS29485 0.12 -0.3
1,972,165 - -1.5
1,972,286 - RR42_RS29485 0.20 +0.9
1,972,287 + RR42_RS29485 0.20 -0.5
1,972,287 + RR42_RS29485 0.20 -0.0
1,972,287 + RR42_RS29485 0.20 +0.2
1,972,287 + RR42_RS29485 0.20 -0.3
1,972,287 + RR42_RS29485 0.20 +2.0
1,972,287 + RR42_RS29485 0.20 -0.4
1,972,287 + RR42_RS29485 0.20 -0.2
1,972,287 + RR42_RS29485 0.20 -1.2
1,972,287 + RR42_RS29485 0.20 -1.5
1,972,287 + RR42_RS29485 0.20 -0.5
1,972,287 + RR42_RS29485 0.20 -0.5
1,972,287 + RR42_RS29485 0.20 -1.5
1,972,287 + RR42_RS29485 0.20 +0.5
1,972,287 + RR42_RS29485 0.20 -1.0
1,972,287 + RR42_RS29485 0.20 -0.0
1,972,287 + RR42_RS29485 0.20 -0.3
1,972,288 - RR42_RS29485 0.20 -0.7
1,972,288 - RR42_RS29485 0.20 -0.7
1,972,288 - RR42_RS29485 0.20 -0.3
1,972,288 - RR42_RS29485 0.20 +0.4
1,972,288 - RR42_RS29485 0.20 -0.5
1,972,288 - RR42_RS29485 0.20 -0.5
1,972,288 - RR42_RS29485 0.20 -0.6
1,972,288 - RR42_RS29485 0.20 +0.4
1,972,288 - RR42_RS29485 0.20 +0.8
1,972,288 - RR42_RS29485 0.20 +1.3
1,972,288 - RR42_RS29485 0.20 -1.0
1,972,288 - RR42_RS29485 0.20 +0.1
1,972,288 - RR42_RS29485 0.20 -0.5
1,972,288 - RR42_RS29485 0.20 -0.8
1,972,291 + RR42_RS29485 0.20 -1.1
1,972,291 + RR42_RS29485 0.20 -0.7
1,972,292 - RR42_RS29485 0.20 +0.2
1,972,292 - RR42_RS29485 0.20 -1.2
1,972,292 - RR42_RS29485 0.20 -0.5
1,972,292 - RR42_RS29485 0.20 -0.1
1,972,292 - RR42_RS29485 0.20 +0.7
1,972,318 + RR42_RS29485 0.22 +1.0
1,972,320 + RR42_RS29485 0.22 +2.6
1,972,320 + RR42_RS29485 0.22 +1.3
1,972,320 + RR42_RS29485 0.22 +0.4
1,972,321 - RR42_RS29485 0.22 +0.5
1,972,321 - RR42_RS29485 0.22 +0.1
1,972,596 + RR42_RS29485 0.39 -1.5
1,973,019 + RR42_RS29485 0.65 +0.4
1,973,020 - RR42_RS29485 0.65 -0.7
1,973,020 - RR42_RS29485 0.65 -0.7
1,973,020 - RR42_RS29485 0.65 +1.3
1,973,296 + RR42_RS29485 0.82 -0.7
1,973,296 + RR42_RS29485 0.82 -0.8
1,973,296 + RR42_RS29485 0.82 -0.9
1,973,296 + RR42_RS29485 0.82 +1.0
1,973,297 - RR42_RS29485 0.82 +0.3
1,973,297 - RR42_RS29485 0.82 -0.5
1,973,297 - RR42_RS29485 0.82 +1.3
1,973,297 - RR42_RS29485 0.82 -1.9
1,973,297 - RR42_RS29485 0.82 -0.5
1,973,297 - RR42_RS29485 0.82 +0.0
1,973,297 - RR42_RS29485 0.82 +3.3
1,973,297 - RR42_RS29485 0.82 +0.3
1,973,297 - RR42_RS29485 0.82 -1.0
1,973,515 - +1.3
1,973,664 + -1.0
1,973,664 + +0.7
1,973,665 - +1.0
1,973,672 + +0.5
1,973,672 + +0.1
1,973,672 + +0.1
1,973,673 - +1.4
1,973,763 - -0.5
1,973,763 - +0.1
1,973,805 + +1.9
1,973,984 - RR42_RS29490 0.50 +0.5
1,974,068 - RR42_RS29490 0.83 -1.3
1,974,107 + +2.0
1,974,108 - -0.3
1,974,146 + +1.6
1,974,168 - +1.3
1,974,212 - -0.5
1,974,279 + RR42_RS29495 0.12 -0.8
1,974,279 + RR42_RS29495 0.12 +0.1
1,974,280 - RR42_RS29495 0.13 -0.7
1,974,313 + RR42_RS29495 0.15 -2.7

Or see this region's nucleotide sequence