Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28785 and RR42_RS28790 overlap by 4 nucleotides RR42_RS28790 and RR42_RS28795 are separated by 346 nucleotides RR42_RS28795 and RR42_RS28800 are separated by 286 nucleotides
RR42_RS28785: RR42_RS28785 - molecular chaperone DnaK, at 1,808,855 to 1,810,702
_RS28785
RR42_RS28790: RR42_RS28790 - hypothetical protein, at 1,810,699 to 1,811,301
_RS28790
RR42_RS28795: RR42_RS28795 - hypothetical protein, at 1,811,648 to 1,811,866
_RS28795
RR42_RS28800: RR42_RS28800 - isocitrate dehydrogenase, at 1,812,153 to 1,814,390
_RS28800
Position (kb)
1811
1812 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1811.355 kb on + strand at 1811.355 kb on + strand at 1811.356 kb on - strand at 1811.574 kb on - strand at 1811.591 kb on - strand at 1811.597 kb on + strand at 1811.597 kb on + strand at 1811.597 kb on + strand at 1811.597 kb on + strand at 1811.598 kb on - strand at 1811.598 kb on - strand at 1811.598 kb on - strand at 1811.601 kb on - strand at 1811.601 kb on - strand at 1811.611 kb on - strand at 1811.701 kb on - strand, within RR42_RS28795 at 1811.701 kb on - strand, within RR42_RS28795 at 1811.724 kb on + strand, within RR42_RS28795 at 1811.725 kb on - strand, within RR42_RS28795 at 1811.735 kb on - strand, within RR42_RS28795 at 1811.735 kb on - strand, within RR42_RS28795 at 1811.765 kb on + strand, within RR42_RS28795 at 1811.766 kb on - strand, within RR42_RS28795 at 1811.766 kb on - strand, within RR42_RS28795 at 1811.766 kb on - strand, within RR42_RS28795 at 1811.779 kb on - strand, within RR42_RS28795 at 1811.779 kb on - strand, within RR42_RS28795 at 1811.779 kb on - strand, within RR42_RS28795 at 1811.864 kb on + strand at 1811.865 kb on - strand at 1811.907 kb on + strand at 1812.129 kb on + strand at 1812.129 kb on + strand at 1812.130 kb on - strand at 1812.148 kb on - strand at 1812.180 kb on + strand at 1812.181 kb on - strand at 1812.300 kb on - strand at 1812.300 kb on - strand at 1812.300 kb on - strand at 1812.300 kb on - strand at 1812.471 kb on + strand, within RR42_RS28800 at 1812.534 kb on + strand, within RR42_RS28800 at 1812.618 kb on + strand, within RR42_RS28800 at 1812.619 kb on - strand, within RR42_RS28800 at 1812.702 kb on + strand, within RR42_RS28800 at 1812.703 kb on - strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.865 kb on - strand, within RR42_RS28800
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,811,355 + -0.0 1,811,355 + +4.0 1,811,356 - +0.5 1,811,574 - +0.7 1,811,591 - -1.1 1,811,597 + -0.7 1,811,597 + -0.1 1,811,597 + -0.8 1,811,597 + +0.7 1,811,598 - +0.4 1,811,598 - -0.3 1,811,598 - -1.3 1,811,601 - +0.2 1,811,601 - +0.0 1,811,611 - +0.4 1,811,701 - RR42_RS28795 0.24 +0.3 1,811,701 - RR42_RS28795 0.24 +0.7 1,811,724 + RR42_RS28795 0.35 +0.1 1,811,725 - RR42_RS28795 0.35 -2.7 1,811,735 - RR42_RS28795 0.40 +0.7 1,811,735 - RR42_RS28795 0.40 +0.8 1,811,765 + RR42_RS28795 0.53 +0.5 1,811,766 - RR42_RS28795 0.54 +0.3 1,811,766 - RR42_RS28795 0.54 +0.3 1,811,766 - RR42_RS28795 0.54 +0.5 1,811,779 - RR42_RS28795 0.60 -0.1 1,811,779 - RR42_RS28795 0.60 +0.8 1,811,779 - RR42_RS28795 0.60 -2.5 1,811,864 + -0.9 1,811,865 - -1.3 1,811,907 + +0.1 1,812,129 + -0.6 1,812,129 + -1.3 1,812,130 - -0.8 1,812,148 - -1.2 1,812,180 + -1.3 1,812,181 - -0.3 1,812,300 - -0.0 1,812,300 - +1.0 1,812,300 - +0.0 1,812,300 - -0.6 1,812,471 + RR42_RS28800 0.14 -0.1 1,812,534 + RR42_RS28800 0.17 -0.7 1,812,618 + RR42_RS28800 0.21 -0.9 1,812,619 - RR42_RS28800 0.21 +0.7 1,812,702 + RR42_RS28800 0.25 -0.1 1,812,703 - RR42_RS28800 0.25 -0.5 1,812,864 + RR42_RS28800 0.32 +0.6 1,812,864 + RR42_RS28800 0.32 -1.1 1,812,864 + RR42_RS28800 0.32 +0.2 1,812,864 + RR42_RS28800 0.32 +0.0 1,812,864 + RR42_RS28800 0.32 -1.8 1,812,864 + RR42_RS28800 0.32 -1.9 1,812,865 - RR42_RS28800 0.32 -1.3
Or see this region's nucleotide sequence