Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28595 and RR42_RS28600 are separated by 41 nucleotides RR42_RS28600 and RR42_RS28605 are separated by 290 nucleotides RR42_RS28605 and RR42_RS28610 are separated by 7 nucleotides
RR42_RS28595: RR42_RS28595 - squalene--hopene cyclase, at 1,770,808 to 1,772,718
_RS28595
RR42_RS28600: RR42_RS28600 - hypothetical protein, at 1,772,760 to 1,773,221
_RS28600
RR42_RS28605: RR42_RS28605 - hypothetical protein, at 1,773,512 to 1,774,969
_RS28605
RR42_RS28610: RR42_RS28610 - acyl-CoA dehydrogenase, at 1,774,977 to 1,776,248
_RS28610
Position (kb)
1773
1774
1775 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1772.526 kb on + strand, within RR42_RS28595 at 1772.554 kb on + strand at 1772.554 kb on + strand at 1772.554 kb on + strand at 1772.555 kb on - strand at 1772.629 kb on + strand at 1772.629 kb on + strand at 1772.629 kb on + strand at 1772.630 kb on - strand at 1772.630 kb on - strand at 1772.630 kb on - strand at 1772.630 kb on - strand at 1772.630 kb on - strand at 1772.633 kb on - strand at 1772.633 kb on - strand at 1772.659 kb on + strand at 1772.660 kb on - strand at 1772.660 kb on - strand at 1772.719 kb on + strand at 1772.719 kb on + strand at 1772.748 kb on + strand at 1772.748 kb on + strand at 1772.799 kb on + strand at 1772.830 kb on + strand, within RR42_RS28600 at 1772.831 kb on - strand, within RR42_RS28600 at 1772.893 kb on + strand, within RR42_RS28600 at 1772.894 kb on - strand, within RR42_RS28600 at 1772.986 kb on + strand, within RR42_RS28600 at 1772.987 kb on - strand, within RR42_RS28600 at 1772.987 kb on - strand, within RR42_RS28600 at 1773.036 kb on - strand, within RR42_RS28600 at 1773.179 kb on + strand at 1773.180 kb on - strand at 1773.390 kb on + strand at 1773.390 kb on + strand at 1773.390 kb on + strand at 1773.390 kb on + strand at 1773.390 kb on + strand at 1773.390 kb on + strand at 1773.390 kb on + strand at 1773.391 kb on - strand at 1773.391 kb on - strand at 1773.391 kb on - strand at 1773.391 kb on - strand at 1773.391 kb on - strand at 1773.391 kb on - strand at 1773.393 kb on - strand at 1773.393 kb on - strand at 1773.393 kb on - strand at 1773.393 kb on - strand at 1773.393 kb on - strand at 1773.393 kb on - strand at 1773.477 kb on + strand at 1774.098 kb on + strand, within RR42_RS28605 at 1774.099 kb on - strand, within RR42_RS28605 at 1774.099 kb on - strand, within RR42_RS28605 at 1774.099 kb on - strand, within RR42_RS28605 at 1774.299 kb on + strand, within RR42_RS28605 at 1774.300 kb on - strand, within RR42_RS28605 at 1774.431 kb on + strand, within RR42_RS28605 at 1774.432 kb on - strand, within RR42_RS28605 at 1774.568 kb on + strand, within RR42_RS28605 at 1774.568 kb on + strand, within RR42_RS28605 at 1774.569 kb on - strand, within RR42_RS28605 at 1774.569 kb on - strand, within RR42_RS28605 at 1774.569 kb on - strand, within RR42_RS28605 at 1774.654 kb on - strand, within RR42_RS28605 at 1774.721 kb on + strand, within RR42_RS28605 at 1774.722 kb on - strand, within RR42_RS28605 at 1774.758 kb on + strand, within RR42_RS28605 at 1774.791 kb on + strand, within RR42_RS28605 at 1774.815 kb on + strand, within RR42_RS28605 at 1775.116 kb on + strand, within RR42_RS28610 at 1775.435 kb on - strand, within RR42_RS28610 at 1775.435 kb on - strand, within RR42_RS28610 at 1775.719 kb on + strand, within RR42_RS28610 at 1775.720 kb on - strand, within RR42_RS28610 at 1775.720 kb on - strand, within RR42_RS28610 at 1775.949 kb on - strand, within RR42_RS28610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,772,526 + RR42_RS28595 0.90 -3.3 1,772,554 + -1.1 1,772,554 + -0.4 1,772,554 + -1.3 1,772,555 - -1.3 1,772,629 + +0.1 1,772,629 + -2.1 1,772,629 + -0.3 1,772,630 - -2.0 1,772,630 - +1.2 1,772,630 - +1.2 1,772,630 - -1.1 1,772,630 - -0.3 1,772,633 - -1.6 1,772,633 - -1.2 1,772,659 + -1.5 1,772,660 - +2.8 1,772,660 - +0.1 1,772,719 + -0.1 1,772,719 + +0.2 1,772,748 + -2.2 1,772,748 + -1.1 1,772,799 + -0.3 1,772,830 + RR42_RS28600 0.15 -0.3 1,772,831 - RR42_RS28600 0.15 -0.1 1,772,893 + RR42_RS28600 0.29 -2.2 1,772,894 - RR42_RS28600 0.29 +0.7 1,772,986 + RR42_RS28600 0.49 -1.3 1,772,987 - RR42_RS28600 0.49 -0.2 1,772,987 - RR42_RS28600 0.49 +0.7 1,773,036 - RR42_RS28600 0.60 -0.5 1,773,179 + -0.7 1,773,180 - -0.2 1,773,390 + -0.3 1,773,390 + +0.3 1,773,390 + -1.6 1,773,390 + +0.7 1,773,390 + -0.9 1,773,390 + -0.9 1,773,390 + -0.3 1,773,391 - +3.4 1,773,391 - -0.1 1,773,391 - +0.6 1,773,391 - -0.8 1,773,391 - -0.7 1,773,391 - -0.6 1,773,393 - -0.2 1,773,393 - +0.4 1,773,393 - -0.8 1,773,393 - +1.1 1,773,393 - -2.2 1,773,393 - -1.1 1,773,477 + +0.9 1,774,098 + RR42_RS28605 0.40 +0.2 1,774,099 - RR42_RS28605 0.40 -0.1 1,774,099 - RR42_RS28605 0.40 -1.1 1,774,099 - RR42_RS28605 0.40 +0.9 1,774,299 + RR42_RS28605 0.54 -1.3 1,774,300 - RR42_RS28605 0.54 +0.5 1,774,431 + RR42_RS28605 0.63 -1.1 1,774,432 - RR42_RS28605 0.63 +0.1 1,774,568 + RR42_RS28605 0.72 +0.1 1,774,568 + RR42_RS28605 0.72 -2.1 1,774,569 - RR42_RS28605 0.72 -0.6 1,774,569 - RR42_RS28605 0.72 +0.3 1,774,569 - RR42_RS28605 0.72 +0.9 1,774,654 - RR42_RS28605 0.78 +0.4 1,774,721 + RR42_RS28605 0.83 +0.5 1,774,722 - RR42_RS28605 0.83 +1.2 1,774,758 + RR42_RS28605 0.85 -1.8 1,774,791 + RR42_RS28605 0.88 -1.1 1,774,815 + RR42_RS28605 0.89 -0.7 1,775,116 + RR42_RS28610 0.11 +0.2 1,775,435 - RR42_RS28610 0.36 -0.5 1,775,435 - RR42_RS28610 0.36 -0.6 1,775,719 + RR42_RS28610 0.58 +0.1 1,775,720 - RR42_RS28610 0.58 +0.2 1,775,720 - RR42_RS28610 0.58 +0.6 1,775,949 - RR42_RS28610 0.76 +0.1
Or see this region's nucleotide sequence