Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28405 and RR42_RS28410 are separated by 31 nucleotides RR42_RS28410 and RR42_RS28415 are separated by 5 nucleotides RR42_RS28415 and RR42_RS28420 are separated by 2 nucleotides
RR42_RS28405: RR42_RS28405 - protocatechuate 3,4-dioxygenase, at 1,733,477 to 1,734,472
_RS28405
RR42_RS28410: RR42_RS28410 - dimethylmenaquinone methyltransferase, at 1,734,504 to 1,735,166
_RS28410
RR42_RS28415: RR42_RS28415 - diguanylate cyclase, at 1,735,172 to 1,735,834
_RS28415
RR42_RS28420: RR42_RS28420 - 4-hydroxy-4-methyl-2-oxoglutarate aldolase, at 1,735,837 to 1,736,499
_RS28420
Position (kb)
1734
1735
1736 Strain fitness (log2 ratio)
-2
-1
0
1 at 1733.634 kb on + strand, within RR42_RS28405 at 1733.635 kb on - strand, within RR42_RS28405 at 1733.687 kb on + strand, within RR42_RS28405 at 1733.819 kb on + strand, within RR42_RS28405 at 1733.820 kb on - strand, within RR42_RS28405 at 1734.401 kb on + strand at 1734.402 kb on - strand at 1734.494 kb on - strand at 1734.526 kb on + strand at 1734.527 kb on - strand at 1734.527 kb on - strand at 1734.581 kb on - strand, within RR42_RS28410 at 1734.890 kb on + strand, within RR42_RS28410 at 1734.911 kb on + strand, within RR42_RS28410 at 1734.990 kb on + strand, within RR42_RS28410 at 1735.143 kb on + strand at 1735.164 kb on + strand at 1735.165 kb on - strand at 1735.320 kb on - strand, within RR42_RS28415 at 1735.331 kb on + strand, within RR42_RS28415 at 1735.332 kb on - strand, within RR42_RS28415 at 1735.370 kb on + strand, within RR42_RS28415 at 1735.371 kb on - strand, within RR42_RS28415 at 1735.411 kb on + strand, within RR42_RS28415 at 1735.412 kb on - strand, within RR42_RS28415 at 1735.533 kb on + strand, within RR42_RS28415 at 1735.533 kb on + strand, within RR42_RS28415 at 1735.608 kb on + strand, within RR42_RS28415 at 1735.609 kb on - strand, within RR42_RS28415 at 1736.048 kb on + strand, within RR42_RS28420 at 1736.048 kb on + strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,733,634 + RR42_RS28405 0.16 +0.1 1,733,635 - RR42_RS28405 0.16 +0.6 1,733,687 + RR42_RS28405 0.21 -0.2 1,733,819 + RR42_RS28405 0.34 +0.6 1,733,820 - RR42_RS28405 0.34 -0.1 1,734,401 + -0.3 1,734,402 - -0.7 1,734,494 - +0.8 1,734,526 + -0.3 1,734,527 - -1.7 1,734,527 - +0.0 1,734,581 - RR42_RS28410 0.12 -0.6 1,734,890 + RR42_RS28410 0.58 -1.7 1,734,911 + RR42_RS28410 0.61 -2.0 1,734,990 + RR42_RS28410 0.73 -0.7 1,735,143 + -0.6 1,735,164 + +0.7 1,735,165 - +0.1 1,735,320 - RR42_RS28415 0.22 +0.0 1,735,331 + RR42_RS28415 0.24 -2.2 1,735,332 - RR42_RS28415 0.24 +0.6 1,735,370 + RR42_RS28415 0.30 -0.1 1,735,371 - RR42_RS28415 0.30 -0.1 1,735,411 + RR42_RS28415 0.36 +0.2 1,735,412 - RR42_RS28415 0.36 +0.5 1,735,533 + RR42_RS28415 0.54 -0.1 1,735,533 + RR42_RS28415 0.54 +0.2 1,735,608 + RR42_RS28415 0.66 -1.7 1,735,609 - RR42_RS28415 0.66 -1.1 1,736,048 + RR42_RS28420 0.32 -0.0 1,736,048 + RR42_RS28420 0.32 -2.3 1,736,049 - RR42_RS28420 0.32 +0.3 1,736,049 - RR42_RS28420 0.32 -1.0 1,736,049 - RR42_RS28420 0.32 -0.7
Or see this region's nucleotide sequence