Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27620 and RR42_RS27625 are separated by 123 nucleotides RR42_RS27625 and RR42_RS27630 are separated by 268 nucleotides RR42_RS27630 and RR42_RS27635 are separated by 454 nucleotides
RR42_RS27620: RR42_RS27620 - AP endonuclease, at 1,560,766 to 1,561,821
_RS27620
RR42_RS27625: RR42_RS27625 - TetR family transcriptional regulator, at 1,561,945 to 1,562,544
_RS27625
RR42_RS27630: RR42_RS27630 - lipase, at 1,562,813 to 1,563,853
_RS27630
RR42_RS27635: RR42_RS27635 - hypothetical protein, at 1,564,308 to 1,564,529
_RS27635
Position (kb)
1562
1563
1564 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1561.905 kb on + strand at 1562.081 kb on - strand, within RR42_RS27625 at 1562.131 kb on + strand, within RR42_RS27625 at 1562.132 kb on - strand, within RR42_RS27625 at 1562.590 kb on - strand at 1562.617 kb on + strand at 1562.617 kb on + strand at 1562.620 kb on - strand at 1563.090 kb on - strand, within RR42_RS27630 at 1563.106 kb on - strand, within RR42_RS27630 at 1563.141 kb on + strand, within RR42_RS27630 at 1563.141 kb on + strand, within RR42_RS27630 at 1563.141 kb on + strand, within RR42_RS27630 at 1563.142 kb on - strand, within RR42_RS27630 at 1563.165 kb on - strand, within RR42_RS27630 at 1563.181 kb on + strand, within RR42_RS27630 at 1563.270 kb on - strand, within RR42_RS27630 at 1563.298 kb on + strand, within RR42_RS27630 at 1563.602 kb on + strand, within RR42_RS27630 at 1563.602 kb on + strand, within RR42_RS27630 at 1563.728 kb on + strand, within RR42_RS27630 at 1563.755 kb on + strand at 1563.755 kb on + strand at 1563.755 kb on + strand at 1563.756 kb on - strand at 1563.756 kb on - strand at 1563.756 kb on - strand at 1563.756 kb on - strand at 1563.756 kb on - strand at 1563.867 kb on + strand at 1563.868 kb on - strand at 1564.262 kb on + strand at 1564.263 kb on - strand at 1564.423 kb on + strand, within RR42_RS27635 at 1564.718 kb on + strand at 1564.718 kb on + strand at 1564.718 kb on + strand at 1564.719 kb on - strand at 1564.780 kb on + strand at 1564.780 kb on + strand at 1564.781 kb on - strand at 1564.781 kb on - strand at 1564.815 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,561,905 + -0.3 1,562,081 - RR42_RS27625 0.23 +1.3 1,562,131 + RR42_RS27625 0.31 -0.6 1,562,132 - RR42_RS27625 0.31 -1.6 1,562,590 - -0.5 1,562,617 + -0.7 1,562,617 + +1.0 1,562,620 - +0.1 1,563,090 - RR42_RS27630 0.27 -1.3 1,563,106 - RR42_RS27630 0.28 -0.8 1,563,141 + RR42_RS27630 0.32 -0.2 1,563,141 + RR42_RS27630 0.32 +1.2 1,563,141 + RR42_RS27630 0.32 -2.2 1,563,142 - RR42_RS27630 0.32 -0.4 1,563,165 - RR42_RS27630 0.34 +0.3 1,563,181 + RR42_RS27630 0.35 +2.3 1,563,270 - RR42_RS27630 0.44 +0.1 1,563,298 + RR42_RS27630 0.47 +0.3 1,563,602 + RR42_RS27630 0.76 +0.7 1,563,602 + RR42_RS27630 0.76 +0.4 1,563,728 + RR42_RS27630 0.88 -1.6 1,563,755 + +0.4 1,563,755 + +0.7 1,563,755 + +1.1 1,563,756 - +0.1 1,563,756 - -1.1 1,563,756 - +0.7 1,563,756 - -0.3 1,563,756 - -1.3 1,563,867 + -0.2 1,563,868 - +0.6 1,564,262 + +0.4 1,564,263 - -0.9 1,564,423 + RR42_RS27635 0.52 +0.1 1,564,718 + -0.1 1,564,718 + +0.9 1,564,718 + -0.8 1,564,719 - -2.2 1,564,780 + -1.5 1,564,780 + +0.1 1,564,781 - +0.2 1,564,781 - +1.3 1,564,815 + -0.5
Or see this region's nucleotide sequence