Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27615 and RR42_RS27620 are separated by 47 nucleotides RR42_RS27620 and RR42_RS27625 are separated by 123 nucleotides RR42_RS27625 and RR42_RS27630 are separated by 268 nucleotides
RR42_RS27615: RR42_RS27615 - alpha/beta hydrolase, at 1,559,831 to 1,560,718
_RS27615
RR42_RS27620: RR42_RS27620 - AP endonuclease, at 1,560,766 to 1,561,821
_RS27620
RR42_RS27625: RR42_RS27625 - TetR family transcriptional regulator, at 1,561,945 to 1,562,544
_RS27625
RR42_RS27630: RR42_RS27630 - lipase, at 1,562,813 to 1,563,853
_RS27630
Position (kb)
1560
1561
1562 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1560.096 kb on + strand, within RR42_RS27615 at 1560.097 kb on - strand, within RR42_RS27615 at 1560.097 kb on - strand, within RR42_RS27615 at 1560.097 kb on - strand, within RR42_RS27615 at 1560.147 kb on + strand, within RR42_RS27615 at 1560.147 kb on + strand, within RR42_RS27615 at 1560.147 kb on + strand, within RR42_RS27615 at 1560.148 kb on - strand, within RR42_RS27615 at 1560.393 kb on + strand, within RR42_RS27615 at 1560.496 kb on - strand, within RR42_RS27615 at 1560.687 kb on + strand at 1560.688 kb on - strand at 1560.944 kb on + strand, within RR42_RS27620 at 1560.950 kb on + strand, within RR42_RS27620 at 1560.950 kb on + strand, within RR42_RS27620 at 1560.950 kb on + strand, within RR42_RS27620 at 1561.050 kb on - strand, within RR42_RS27620 at 1561.050 kb on - strand, within RR42_RS27620 at 1561.130 kb on + strand, within RR42_RS27620 at 1561.130 kb on + strand, within RR42_RS27620 at 1561.140 kb on - strand, within RR42_RS27620 at 1561.140 kb on - strand, within RR42_RS27620 at 1561.386 kb on - strand, within RR42_RS27620 at 1561.644 kb on - strand, within RR42_RS27620 at 1561.644 kb on - strand, within RR42_RS27620 at 1561.905 kb on + strand at 1562.081 kb on - strand, within RR42_RS27625 at 1562.131 kb on + strand, within RR42_RS27625 at 1562.132 kb on - strand, within RR42_RS27625 at 1562.590 kb on - strand at 1562.617 kb on + strand at 1562.617 kb on + strand at 1562.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,560,096 + RR42_RS27615 0.30 +0.3 1,560,097 - RR42_RS27615 0.30 -0.1 1,560,097 - RR42_RS27615 0.30 -1.1 1,560,097 - RR42_RS27615 0.30 +0.2 1,560,147 + RR42_RS27615 0.36 -0.7 1,560,147 + RR42_RS27615 0.36 -0.3 1,560,147 + RR42_RS27615 0.36 +0.9 1,560,148 - RR42_RS27615 0.36 -1.0 1,560,393 + RR42_RS27615 0.63 -0.0 1,560,496 - RR42_RS27615 0.75 -0.1 1,560,687 + -1.4 1,560,688 - +0.0 1,560,944 + RR42_RS27620 0.17 +0.3 1,560,950 + RR42_RS27620 0.17 +1.8 1,560,950 + RR42_RS27620 0.17 +0.1 1,560,950 + RR42_RS27620 0.17 +0.0 1,561,050 - RR42_RS27620 0.27 -0.4 1,561,050 - RR42_RS27620 0.27 -0.2 1,561,130 + RR42_RS27620 0.34 +0.3 1,561,130 + RR42_RS27620 0.34 -0.4 1,561,140 - RR42_RS27620 0.35 -0.7 1,561,140 - RR42_RS27620 0.35 -0.8 1,561,386 - RR42_RS27620 0.59 +0.6 1,561,644 - RR42_RS27620 0.83 -0.4 1,561,644 - RR42_RS27620 0.83 -1.9 1,561,905 + -0.3 1,562,081 - RR42_RS27625 0.23 +1.3 1,562,131 + RR42_RS27625 0.31 -0.6 1,562,132 - RR42_RS27625 0.31 -1.6 1,562,590 - -0.5 1,562,617 + -0.7 1,562,617 + +1.0 1,562,620 - +0.1
Or see this region's nucleotide sequence