Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27055 and RR42_RS27060 overlap by 8 nucleotides RR42_RS27060 and RR42_RS27065 are separated by 393 nucleotides RR42_RS27065 and RR42_RS27070 are separated by 27 nucleotides
RR42_RS27055: RR42_RS27055 - membrane protein, at 1,420,971 to 1,424,111
_RS27055
RR42_RS27060: RR42_RS27060 - cation transporter, at 1,424,104 to 1,425,243
_RS27060
RR42_RS27065: RR42_RS27065 - hypothetical protein, at 1,425,637 to 1,425,993
_RS27065
RR42_RS27070: RR42_RS27070 - membrane protein, at 1,426,021 to 1,426,725
_RS27070
Position (kb) 1424
1425
1426 Strain fitness (log2 ratio) -2
-1
0
1 at 1423.439 kb on - strand, within RR42_RS27055 at 1423.439 kb on - strand, within RR42_RS27055 at 1423.694 kb on - strand, within RR42_RS27055 at 1423.700 kb on - strand, within RR42_RS27055 at 1423.977 kb on - strand at 1424.026 kb on + strand at 1424.114 kb on - strand at 1424.114 kb on - strand at 1424.736 kb on - strand, within RR42_RS27060 at 1425.123 kb on + strand, within RR42_RS27060 at 1425.124 kb on - strand, within RR42_RS27060 at 1425.189 kb on + strand at 1425.881 kb on + strand, within RR42_RS27065 at 1425.993 kb on + strand at 1426.150 kb on + strand, within RR42_RS27070 at 1426.150 kb on + strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,423,439 - RR42_RS27055 0.79 +0.5 1,423,439 - RR42_RS27055 0.79 -0.4 1,423,694 - RR42_RS27055 0.87 +0.7 1,423,700 - RR42_RS27055 0.87 -0.5 1,423,977 - +0.4 1,424,026 + +0.1 1,424,114 - -0.2 1,424,114 - +0.3 1,424,736 - RR42_RS27060 0.55 -0.7 1,425,123 + RR42_RS27060 0.89 +0.8 1,425,124 - RR42_RS27060 0.89 -0.7 1,425,189 + -2.7 1,425,881 + RR42_RS27065 0.68 -2.0 1,425,993 + -0.1 1,426,150 + RR42_RS27070 0.18 +0.3 1,426,150 + RR42_RS27070 0.18 +0.4 1,426,151 - RR42_RS27070 0.18 +0.6 1,426,151 - RR42_RS27070 0.18 -1.1 1,426,151 - RR42_RS27070 0.18 +0.4
Or see this region's nucleotide sequence