Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26240 and RR42_RS26245 are separated by 106 nucleotides RR42_RS26245 and RR42_RS26250 are separated by 26 nucleotides
RR42_RS26240: RR42_RS26240 - 4-aminobutyrate aminotransferase, at 1,244,565 to 1,245,827
_RS26240
RR42_RS26245: RR42_RS26245 - thiamine pyrophosphate-binding protein, at 1,245,934 to 1,247,661
_RS26245
RR42_RS26250: RR42_RS26250 - allantoate amidohydrolase, at 1,247,688 to 1,248,935
_RS26250
Position (kb) 1245
1246
1247
1248 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1245.024 kb on + strand, within RR42_RS26240 at 1245.024 kb on + strand, within RR42_RS26240 at 1245.025 kb on - strand, within RR42_RS26240 at 1245.025 kb on - strand, within RR42_RS26240 at 1245.078 kb on + strand, within RR42_RS26240 at 1245.330 kb on + strand, within RR42_RS26240 at 1245.331 kb on - strand, within RR42_RS26240 at 1245.331 kb on - strand, within RR42_RS26240 at 1245.732 kb on + strand at 1245.733 kb on - strand at 1245.733 kb on - strand at 1246.054 kb on + strand at 1246.054 kb on + strand at 1246.054 kb on + strand at 1246.055 kb on - strand at 1246.055 kb on - strand at 1246.055 kb on - strand at 1246.136 kb on - strand, within RR42_RS26245 at 1246.136 kb on - strand, within RR42_RS26245 at 1246.136 kb on - strand, within RR42_RS26245 at 1246.292 kb on - strand, within RR42_RS26245 at 1246.760 kb on + strand, within RR42_RS26245 at 1246.822 kb on + strand, within RR42_RS26245 at 1246.822 kb on + strand, within RR42_RS26245 at 1246.823 kb on - strand, within RR42_RS26245 at 1246.823 kb on - strand, within RR42_RS26245 at 1246.870 kb on + strand, within RR42_RS26245 at 1247.116 kb on - strand, within RR42_RS26245 at 1247.134 kb on + strand, within RR42_RS26245 at 1247.134 kb on + strand, within RR42_RS26245 at 1247.135 kb on - strand, within RR42_RS26245 at 1247.135 kb on - strand, within RR42_RS26245 at 1247.135 kb on - strand, within RR42_RS26245 at 1247.135 kb on - strand, within RR42_RS26245 at 1247.135 kb on - strand, within RR42_RS26245 at 1247.135 kb on - strand, within RR42_RS26245 at 1247.143 kb on + strand, within RR42_RS26245 at 1247.144 kb on - strand, within RR42_RS26245 at 1247.144 kb on - strand, within RR42_RS26245 at 1247.144 kb on - strand, within RR42_RS26245 at 1247.144 kb on - strand, within RR42_RS26245 at 1247.156 kb on - strand, within RR42_RS26245 at 1247.156 kb on - strand, within RR42_RS26245 at 1247.159 kb on - strand, within RR42_RS26245 at 1247.272 kb on + strand, within RR42_RS26245 at 1247.330 kb on - strand, within RR42_RS26245 at 1247.578 kb on + strand at 1247.578 kb on + strand at 1247.579 kb on - strand at 1247.659 kb on + strand at 1247.660 kb on - strand at 1247.660 kb on - strand at 1247.877 kb on - strand, within RR42_RS26250 at 1247.877 kb on - strand, within RR42_RS26250 at 1247.976 kb on + strand, within RR42_RS26250 at 1247.977 kb on - strand, within RR42_RS26250 at 1247.977 kb on - strand, within RR42_RS26250 at 1248.240 kb on + strand, within RR42_RS26250 at 1248.254 kb on + strand, within RR42_RS26250 at 1248.402 kb on + strand, within RR42_RS26250 at 1248.403 kb on - strand, within RR42_RS26250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,245,024 + RR42_RS26240 0.36 +0.6 1,245,024 + RR42_RS26240 0.36 -0.7 1,245,025 - RR42_RS26240 0.36 -2.4 1,245,025 - RR42_RS26240 0.36 +0.1 1,245,078 + RR42_RS26240 0.41 -0.3 1,245,330 + RR42_RS26240 0.61 +0.0 1,245,331 - RR42_RS26240 0.61 -0.6 1,245,331 - RR42_RS26240 0.61 -0.5 1,245,732 + -0.1 1,245,733 - -0.8 1,245,733 - +0.3 1,246,054 + -0.2 1,246,054 + -0.2 1,246,054 + +0.5 1,246,055 - +0.6 1,246,055 - -0.4 1,246,055 - +2.3 1,246,136 - RR42_RS26245 0.12 -0.4 1,246,136 - RR42_RS26245 0.12 +0.7 1,246,136 - RR42_RS26245 0.12 +0.1 1,246,292 - RR42_RS26245 0.21 -0.0 1,246,760 + RR42_RS26245 0.48 +0.4 1,246,822 + RR42_RS26245 0.51 -0.5 1,246,822 + RR42_RS26245 0.51 -1.9 1,246,823 - RR42_RS26245 0.51 +1.1 1,246,823 - RR42_RS26245 0.51 +0.1 1,246,870 + RR42_RS26245 0.54 +0.0 1,247,116 - RR42_RS26245 0.68 +0.5 1,247,134 + RR42_RS26245 0.69 -0.1 1,247,134 + RR42_RS26245 0.69 +0.1 1,247,135 - RR42_RS26245 0.70 +0.3 1,247,135 - RR42_RS26245 0.70 -0.2 1,247,135 - RR42_RS26245 0.70 +1.1 1,247,135 - RR42_RS26245 0.70 +0.7 1,247,135 - RR42_RS26245 0.70 -0.4 1,247,135 - RR42_RS26245 0.70 +1.9 1,247,143 + RR42_RS26245 0.70 +0.6 1,247,144 - RR42_RS26245 0.70 -0.5 1,247,144 - RR42_RS26245 0.70 +0.6 1,247,144 - RR42_RS26245 0.70 +1.3 1,247,144 - RR42_RS26245 0.70 -0.2 1,247,156 - RR42_RS26245 0.71 -0.6 1,247,156 - RR42_RS26245 0.71 +0.6 1,247,159 - RR42_RS26245 0.71 +1.6 1,247,272 + RR42_RS26245 0.77 -1.2 1,247,330 - RR42_RS26245 0.81 -1.0 1,247,578 + -0.3 1,247,578 + +0.9 1,247,579 - -1.0 1,247,659 + +0.4 1,247,660 - +1.0 1,247,660 - -1.0 1,247,877 - RR42_RS26250 0.15 +0.5 1,247,877 - RR42_RS26250 0.15 -0.7 1,247,976 + RR42_RS26250 0.23 -0.5 1,247,977 - RR42_RS26250 0.23 +0.0 1,247,977 - RR42_RS26250 0.23 -1.7 1,248,240 + RR42_RS26250 0.44 -0.2 1,248,254 + RR42_RS26250 0.45 +1.0 1,248,402 + RR42_RS26250 0.57 +0.6 1,248,403 - RR42_RS26250 0.57 -0.8
Or see this region's nucleotide sequence