Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25605 and RR42_RS25610 are separated by 148 nucleotides RR42_RS25610 and RR42_RS25615 are separated by 133 nucleotides RR42_RS25615 and RR42_RS25620 are separated by 6 nucleotides
RR42_RS25605: RR42_RS25605 - N-ethylmaleimide reductase, at 1,088,586 to 1,089,680
_RS25605
RR42_RS25610: RR42_RS25610 - alpha/beta hydrolase, at 1,089,829 to 1,090,668
_RS25610
RR42_RS25615: RR42_RS25615 - TetR family transcriptional regulator, at 1,090,802 to 1,091,383
_RS25615
RR42_RS25620: RR42_RS25620 - hypothetical protein, at 1,091,390 to 1,091,926
_RS25620
Position (kb)
1089
1090
1091 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1088.899 kb on + strand, within RR42_RS25605 at 1088.935 kb on + strand, within RR42_RS25605 at 1089.457 kb on + strand, within RR42_RS25605 at 1089.457 kb on + strand, within RR42_RS25605 at 1089.547 kb on + strand, within RR42_RS25605 at 1089.550 kb on + strand, within RR42_RS25605 at 1089.550 kb on + strand, within RR42_RS25605 at 1089.551 kb on - strand, within RR42_RS25605 at 1089.630 kb on + strand at 1089.652 kb on + strand at 1089.827 kb on + strand at 1089.827 kb on + strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.828 kb on - strand at 1089.830 kb on + strand at 1089.831 kb on - strand at 1089.875 kb on + strand at 1089.882 kb on - strand at 1089.909 kb on - strand at 1089.909 kb on - strand at 1089.947 kb on + strand, within RR42_RS25610 at 1089.947 kb on + strand, within RR42_RS25610 at 1089.998 kb on - strand, within RR42_RS25610 at 1090.025 kb on + strand, within RR42_RS25610 at 1090.031 kb on + strand, within RR42_RS25610 at 1090.031 kb on + strand, within RR42_RS25610 at 1090.031 kb on + strand, within RR42_RS25610 at 1090.061 kb on + strand, within RR42_RS25610 at 1090.197 kb on - strand, within RR42_RS25610 at 1090.197 kb on - strand, within RR42_RS25610 at 1090.197 kb on - strand, within RR42_RS25610 at 1090.197 kb on - strand, within RR42_RS25610 at 1090.294 kb on + strand, within RR42_RS25610 at 1090.294 kb on + strand, within RR42_RS25610 at 1090.294 kb on + strand, within RR42_RS25610 at 1090.450 kb on - strand, within RR42_RS25610 at 1090.673 kb on + strand at 1090.673 kb on + strand at 1090.673 kb on + strand at 1090.674 kb on - strand at 1090.674 kb on - strand at 1090.757 kb on - strand at 1090.758 kb on + strand at 1090.758 kb on + strand at 1090.758 kb on + strand at 1090.769 kb on + strand at 1090.781 kb on + strand at 1090.782 kb on - strand at 1090.866 kb on + strand, within RR42_RS25615 at 1090.866 kb on + strand, within RR42_RS25615 at 1090.866 kb on + strand, within RR42_RS25615 at 1090.867 kb on - strand, within RR42_RS25615 at 1090.944 kb on - strand, within RR42_RS25615 at 1091.055 kb on - strand, within RR42_RS25615 at 1091.100 kb on + strand, within RR42_RS25615 at 1091.212 kb on + strand, within RR42_RS25615 at 1091.381 kb on + strand at 1091.382 kb on - strand at 1091.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,088,899 + RR42_RS25605 0.29 -0.3 1,088,935 + RR42_RS25605 0.32 -0.8 1,089,457 + RR42_RS25605 0.80 -0.3 1,089,457 + RR42_RS25605 0.80 -0.3 1,089,547 + RR42_RS25605 0.88 -0.1 1,089,550 + RR42_RS25605 0.88 -0.3 1,089,550 + RR42_RS25605 0.88 -1.0 1,089,551 - RR42_RS25605 0.88 +0.5 1,089,630 + +0.2 1,089,652 + +0.2 1,089,827 + -0.9 1,089,827 + -0.1 1,089,828 - +0.3 1,089,828 - -1.3 1,089,828 - +0.4 1,089,828 - +1.3 1,089,828 - -0.7 1,089,828 - +0.5 1,089,828 - -1.1 1,089,828 - +0.5 1,089,830 + +1.3 1,089,831 - -0.7 1,089,875 + -0.2 1,089,882 - -1.3 1,089,909 - -1.3 1,089,909 - +0.6 1,089,947 + RR42_RS25610 0.14 +0.5 1,089,947 + RR42_RS25610 0.14 +0.6 1,089,998 - RR42_RS25610 0.20 +0.2 1,090,025 + RR42_RS25610 0.23 +0.1 1,090,031 + RR42_RS25610 0.24 -0.9 1,090,031 + RR42_RS25610 0.24 +0.5 1,090,031 + RR42_RS25610 0.24 +0.5 1,090,061 + RR42_RS25610 0.28 +1.3 1,090,197 - RR42_RS25610 0.44 -0.2 1,090,197 - RR42_RS25610 0.44 +0.5 1,090,197 - RR42_RS25610 0.44 -2.9 1,090,197 - RR42_RS25610 0.44 -1.6 1,090,294 + RR42_RS25610 0.55 -0.4 1,090,294 + RR42_RS25610 0.55 +1.2 1,090,294 + RR42_RS25610 0.55 -1.5 1,090,450 - RR42_RS25610 0.74 +0.6 1,090,673 + -2.5 1,090,673 + -1.3 1,090,673 + -0.2 1,090,674 - -1.2 1,090,674 - +1.0 1,090,757 - -2.4 1,090,758 + +0.3 1,090,758 + -0.9 1,090,758 + -1.7 1,090,769 + +2.9 1,090,781 + -0.6 1,090,782 - -2.1 1,090,866 + RR42_RS25615 0.11 -0.1 1,090,866 + RR42_RS25615 0.11 +0.7 1,090,866 + RR42_RS25615 0.11 +0.8 1,090,867 - RR42_RS25615 0.11 -0.1 1,090,944 - RR42_RS25615 0.24 +0.7 1,091,055 - RR42_RS25615 0.43 +0.5 1,091,100 + RR42_RS25615 0.51 +0.1 1,091,212 + RR42_RS25615 0.70 +0.4 1,091,381 + +1.3 1,091,382 - +1.3 1,091,382 - +0.8
Or see this region's nucleotide sequence