Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25475 and RR42_RS25480 are separated by 11 nucleotides RR42_RS25480 and RR42_RS25485 are separated by 16 nucleotides RR42_RS25485 and RR42_RS25490 are separated by 30 nucleotides
RR42_RS25475: RR42_RS25475 - peptidase S15, at 1,062,110 to 1,063,024
_RS25475
RR42_RS25480: RR42_RS25480 - enoyl-CoA hydratase, at 1,063,036 to 1,063,845
_RS25480
RR42_RS25485: RR42_RS25485 - hypothetical protein, at 1,063,862 to 1,064,335
_RS25485
RR42_RS25490: RR42_RS25490 - ABC transporter substrate-binding protein, at 1,064,366 to 1,065,352
_RS25490
Position (kb)
1063
1064
1065 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1062.894 kb on + strand, within RR42_RS25475 at 1062.894 kb on + strand, within RR42_RS25475 at 1063.005 kb on + strand at 1063.005 kb on + strand at 1063.005 kb on + strand at 1063.006 kb on - strand at 1063.006 kb on - strand at 1063.402 kb on + strand, within RR42_RS25480 at 1063.444 kb on - strand, within RR42_RS25480 at 1063.515 kb on - strand, within RR42_RS25480 at 1063.739 kb on + strand, within RR42_RS25480 at 1063.740 kb on - strand, within RR42_RS25480 at 1063.894 kb on + strand at 1064.066 kb on + strand, within RR42_RS25485 at 1064.067 kb on - strand, within RR42_RS25485 at 1064.074 kb on + strand, within RR42_RS25485 at 1064.075 kb on - strand, within RR42_RS25485 at 1064.082 kb on + strand, within RR42_RS25485 at 1064.196 kb on + strand, within RR42_RS25485 at 1064.196 kb on + strand, within RR42_RS25485 at 1064.259 kb on + strand, within RR42_RS25485 at 1064.259 kb on + strand, within RR42_RS25485 at 1064.259 kb on + strand, within RR42_RS25485 at 1064.259 kb on + strand, within RR42_RS25485 at 1064.260 kb on - strand, within RR42_RS25485 at 1064.260 kb on - strand, within RR42_RS25485 at 1064.260 kb on - strand, within RR42_RS25485 at 1064.278 kb on + strand, within RR42_RS25485 at 1064.394 kb on - strand at 1064.428 kb on - strand at 1064.458 kb on - strand at 1064.911 kb on - strand, within RR42_RS25490 at 1064.911 kb on - strand, within RR42_RS25490 at 1065.018 kb on + strand, within RR42_RS25490 at 1065.019 kb on - strand, within RR42_RS25490 at 1065.033 kb on + strand, within RR42_RS25490 at 1065.033 kb on + strand, within RR42_RS25490 at 1065.084 kb on + strand, within RR42_RS25490 at 1065.267 kb on + strand at 1065.271 kb on + strand at 1065.271 kb on + strand at 1065.271 kb on + strand at 1065.272 kb on - strand at 1065.272 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,062,894 + RR42_RS25475 0.86 -0.7 1,062,894 + RR42_RS25475 0.86 -1.1 1,063,005 + +0.8 1,063,005 + +0.7 1,063,005 + +1.0 1,063,006 - +0.4 1,063,006 - +1.3 1,063,402 + RR42_RS25480 0.45 -0.9 1,063,444 - RR42_RS25480 0.50 +0.2 1,063,515 - RR42_RS25480 0.59 -0.2 1,063,739 + RR42_RS25480 0.87 +0.6 1,063,740 - RR42_RS25480 0.87 -0.0 1,063,894 + +0.3 1,064,066 + RR42_RS25485 0.43 +2.6 1,064,067 - RR42_RS25485 0.43 +0.5 1,064,074 + RR42_RS25485 0.45 +0.3 1,064,075 - RR42_RS25485 0.45 +1.3 1,064,082 + RR42_RS25485 0.46 +0.1 1,064,196 + RR42_RS25485 0.70 -1.2 1,064,196 + RR42_RS25485 0.70 -0.7 1,064,259 + RR42_RS25485 0.84 -0.4 1,064,259 + RR42_RS25485 0.84 -0.3 1,064,259 + RR42_RS25485 0.84 -1.0 1,064,259 + RR42_RS25485 0.84 -0.3 1,064,260 - RR42_RS25485 0.84 -1.5 1,064,260 - RR42_RS25485 0.84 +0.3 1,064,260 - RR42_RS25485 0.84 -0.3 1,064,278 + RR42_RS25485 0.88 -1.2 1,064,394 - +0.7 1,064,428 - +0.1 1,064,458 - -2.1 1,064,911 - RR42_RS25490 0.55 -0.9 1,064,911 - RR42_RS25490 0.55 -0.3 1,065,018 + RR42_RS25490 0.66 +1.4 1,065,019 - RR42_RS25490 0.66 +0.1 1,065,033 + RR42_RS25490 0.68 -0.1 1,065,033 + RR42_RS25490 0.68 -1.2 1,065,084 + RR42_RS25490 0.73 -0.9 1,065,267 + -0.3 1,065,271 + +0.0 1,065,271 + -0.5 1,065,271 + -0.8 1,065,272 - +0.7 1,065,272 - +0.2
Or see this region's nucleotide sequence