Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25235 and RR42_RS25240 are separated by 49 nucleotides RR42_RS25240 and RR42_RS25245 are separated by 164 nucleotides RR42_RS25245 and RR42_RS25250 are separated by 27 nucleotides
RR42_RS25235: RR42_RS25235 - DSBA oxidoreductase, at 1,002,729 to 1,003,331
_RS25235
RR42_RS25240: RR42_RS25240 - nitronate monooxygenase, at 1,003,381 to 1,004,388
_RS25240
RR42_RS25245: RR42_RS25245 - Asp/Glu/hydantoin racemase, at 1,004,553 to 1,005,209
_RS25245
RR42_RS25250: RR42_RS25250 - ABC transporter substrate-binding protein, at 1,005,237 to 1,006,223
_RS25250
Position (kb)
1003
1004
1005 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1002.513 kb on + strand at 1002.514 kb on - strand at 1002.514 kb on - strand at 1002.517 kb on + strand at 1002.517 kb on + strand at 1002.517 kb on + strand at 1002.552 kb on - strand at 1002.552 kb on - strand at 1002.653 kb on + strand at 1002.654 kb on - strand at 1002.791 kb on + strand, within RR42_RS25235 at 1002.791 kb on + strand, within RR42_RS25235 at 1002.791 kb on + strand, within RR42_RS25235 at 1002.791 kb on + strand, within RR42_RS25235 at 1002.792 kb on - strand, within RR42_RS25235 at 1002.792 kb on - strand, within RR42_RS25235 at 1002.792 kb on - strand, within RR42_RS25235 at 1003.006 kb on + strand, within RR42_RS25235 at 1003.039 kb on - strand, within RR42_RS25235 at 1003.099 kb on + strand, within RR42_RS25235 at 1003.148 kb on - strand, within RR42_RS25235 at 1003.190 kb on + strand, within RR42_RS25235 at 1003.225 kb on + strand, within RR42_RS25235 at 1003.226 kb on - strand, within RR42_RS25235 at 1003.367 kb on + strand at 1003.368 kb on - strand at 1003.368 kb on - strand at 1003.368 kb on - strand at 1003.368 kb on - strand at 1004.022 kb on + strand, within RR42_RS25240 at 1004.022 kb on + strand, within RR42_RS25240 at 1004.023 kb on - strand, within RR42_RS25240 at 1004.023 kb on - strand, within RR42_RS25240 at 1004.070 kb on - strand, within RR42_RS25240 at 1004.070 kb on - strand, within RR42_RS25240 at 1004.135 kb on + strand, within RR42_RS25240 at 1004.135 kb on + strand, within RR42_RS25240 at 1004.136 kb on - strand, within RR42_RS25240 at 1004.402 kb on - strand at 1004.463 kb on - strand at 1004.463 kb on - strand at 1004.496 kb on - strand at 1004.635 kb on + strand, within RR42_RS25245 at 1004.635 kb on + strand, within RR42_RS25245 at 1004.635 kb on + strand, within RR42_RS25245 at 1004.636 kb on - strand, within RR42_RS25245 at 1004.636 kb on - strand, within RR42_RS25245 at 1004.758 kb on - strand, within RR42_RS25245 at 1004.999 kb on + strand, within RR42_RS25245 at 1004.999 kb on + strand, within RR42_RS25245 at 1005.000 kb on - strand, within RR42_RS25245 at 1005.043 kb on + strand, within RR42_RS25245 at 1005.043 kb on + strand, within RR42_RS25245 at 1005.184 kb on + strand at 1005.254 kb on + strand at 1005.255 kb on - strand at 1005.255 kb on - strand at 1005.255 kb on - strand at 1005.291 kb on + strand at 1005.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,002,513 + -1.2 1,002,514 - +0.6 1,002,514 - +0.9 1,002,517 + +0.2 1,002,517 + +0.8 1,002,517 + -0.3 1,002,552 - -0.3 1,002,552 - -0.8 1,002,653 + +1.0 1,002,654 - +0.3 1,002,791 + RR42_RS25235 0.10 -0.5 1,002,791 + RR42_RS25235 0.10 +1.8 1,002,791 + RR42_RS25235 0.10 +1.5 1,002,791 + RR42_RS25235 0.10 -1.5 1,002,792 - RR42_RS25235 0.10 +0.6 1,002,792 - RR42_RS25235 0.10 -0.2 1,002,792 - RR42_RS25235 0.10 +0.0 1,003,006 + RR42_RS25235 0.46 +0.8 1,003,039 - RR42_RS25235 0.51 +0.4 1,003,099 + RR42_RS25235 0.61 -0.4 1,003,148 - RR42_RS25235 0.69 -0.4 1,003,190 + RR42_RS25235 0.76 -1.0 1,003,225 + RR42_RS25235 0.82 -0.2 1,003,226 - RR42_RS25235 0.82 +0.5 1,003,367 + +0.1 1,003,368 - +0.4 1,003,368 - -0.9 1,003,368 - +1.3 1,003,368 - -0.1 1,004,022 + RR42_RS25240 0.64 -0.2 1,004,022 + RR42_RS25240 0.64 -1.3 1,004,023 - RR42_RS25240 0.64 -0.1 1,004,023 - RR42_RS25240 0.64 -0.3 1,004,070 - RR42_RS25240 0.68 -0.1 1,004,070 - RR42_RS25240 0.68 -0.1 1,004,135 + RR42_RS25240 0.75 +2.8 1,004,135 + RR42_RS25240 0.75 -0.1 1,004,136 - RR42_RS25240 0.75 +0.2 1,004,402 - +1.1 1,004,463 - -0.3 1,004,463 - +0.7 1,004,496 - +0.4 1,004,635 + RR42_RS25245 0.12 -2.0 1,004,635 + RR42_RS25245 0.12 +0.4 1,004,635 + RR42_RS25245 0.12 +0.2 1,004,636 - RR42_RS25245 0.13 +0.7 1,004,636 - RR42_RS25245 0.13 +1.3 1,004,758 - RR42_RS25245 0.31 -0.9 1,004,999 + RR42_RS25245 0.68 -0.3 1,004,999 + RR42_RS25245 0.68 -0.7 1,005,000 - RR42_RS25245 0.68 -0.0 1,005,043 + RR42_RS25245 0.75 +1.1 1,005,043 + RR42_RS25245 0.75 -0.5 1,005,184 + -0.8 1,005,254 + -0.7 1,005,255 - -0.7 1,005,255 - -0.4 1,005,255 - +0.1 1,005,291 + -0.3 1,005,291 + +0.1
Or see this region's nucleotide sequence