Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24560 and RR42_RS24565 are separated by 208 nucleotides RR42_RS24565 and RR42_RS24570 are separated by 13 nucleotides
RR42_RS24560: RR42_RS24560 - alcohol dehydrogenase, at 842,443 to 843,612
_RS24560
RR42_RS24565: RR42_RS24565 - monooxygenase, at 843,821 to 845,275
_RS24565
RR42_RS24570: RR42_RS24570 - alpha/beta hydrolase, at 845,289 to 846,266
_RS24570
Position (kb) 843
844
845
846 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 842.902 kb on - strand, within RR42_RS24560 at 842.902 kb on - strand, within RR42_RS24560 at 842.960 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.125 kb on + strand, within RR42_RS24560 at 843.141 kb on - strand, within RR42_RS24560 at 843.257 kb on - strand, within RR42_RS24560 at 843.427 kb on - strand, within RR42_RS24560 at 843.546 kb on - strand at 843.607 kb on + strand at 843.746 kb on + strand at 843.746 kb on + strand at 843.747 kb on - strand at 843.747 kb on - strand at 843.763 kb on - strand at 843.840 kb on - strand at 843.840 kb on - strand at 843.972 kb on - strand, within RR42_RS24565 at 844.007 kb on + strand, within RR42_RS24565 at 844.008 kb on - strand, within RR42_RS24565 at 844.049 kb on + strand, within RR42_RS24565 at 844.076 kb on + strand, within RR42_RS24565 at 844.292 kb on + strand, within RR42_RS24565 at 844.293 kb on - strand, within RR42_RS24565 at 844.293 kb on - strand, within RR42_RS24565 at 844.379 kb on + strand, within RR42_RS24565 at 844.380 kb on - strand, within RR42_RS24565 at 844.670 kb on + strand, within RR42_RS24565 at 844.670 kb on + strand, within RR42_RS24565 at 844.671 kb on - strand, within RR42_RS24565 at 844.710 kb on - strand, within RR42_RS24565 at 844.710 kb on - strand, within RR42_RS24565 at 844.925 kb on + strand, within RR42_RS24565 at 844.970 kb on + strand, within RR42_RS24565 at 844.970 kb on + strand, within RR42_RS24565 at 844.971 kb on - strand, within RR42_RS24565 at 845.033 kb on + strand, within RR42_RS24565 at 845.034 kb on - strand, within RR42_RS24565 at 845.034 kb on - strand, within RR42_RS24565 at 845.034 kb on - strand, within RR42_RS24565 at 845.111 kb on + strand, within RR42_RS24565 at 845.111 kb on + strand, within RR42_RS24565 at 845.112 kb on - strand, within RR42_RS24565 at 845.112 kb on - strand, within RR42_RS24565 at 845.112 kb on - strand, within RR42_RS24565 at 845.112 kb on - strand, within RR42_RS24565 at 845.174 kb on + strand at 845.174 kb on + strand at 845.175 kb on - strand at 845.318 kb on - strand at 845.382 kb on + strand at 845.674 kb on - strand, within RR42_RS24570 at 845.744 kb on - strand, within RR42_RS24570 at 845.813 kb on + strand, within RR42_RS24570 at 845.853 kb on + strand, within RR42_RS24570 at 845.854 kb on - strand, within RR42_RS24570 at 845.864 kb on - strand, within RR42_RS24570 at 845.864 kb on - strand, within RR42_RS24570 at 845.864 kb on - strand, within RR42_RS24570 at 845.898 kb on - strand, within RR42_RS24570 at 845.898 kb on - strand, within RR42_RS24570 at 845.980 kb on - strand, within RR42_RS24570 at 846.028 kb on + strand, within RR42_RS24570 at 846.062 kb on + strand, within RR42_RS24570 at 846.108 kb on + strand, within RR42_RS24570 at 846.220 kb on + strand at 846.235 kb on + strand at 846.236 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 842,902 - RR42_RS24560 0.39 +0.5 842,902 - RR42_RS24560 0.39 +1.3 842,960 - RR42_RS24560 0.44 -2.5 843,062 - RR42_RS24560 0.53 +0.3 843,062 - RR42_RS24560 0.53 -0.7 843,062 - RR42_RS24560 0.53 +0.8 843,125 + RR42_RS24560 0.58 -0.4 843,141 - RR42_RS24560 0.60 -1.4 843,257 - RR42_RS24560 0.70 +0.2 843,427 - RR42_RS24560 0.84 +0.1 843,546 - +0.5 843,607 + -0.1 843,746 + +0.1 843,746 + -0.3 843,747 - -1.0 843,747 - +0.2 843,763 - +0.1 843,840 - -0.0 843,840 - -0.7 843,972 - RR42_RS24565 0.10 -1.0 844,007 + RR42_RS24565 0.13 +0.7 844,008 - RR42_RS24565 0.13 -0.7 844,049 + RR42_RS24565 0.16 -0.2 844,076 + RR42_RS24565 0.18 -3.6 844,292 + RR42_RS24565 0.32 -1.3 844,293 - RR42_RS24565 0.32 -0.6 844,293 - RR42_RS24565 0.32 +0.2 844,379 + RR42_RS24565 0.38 -2.0 844,380 - RR42_RS24565 0.38 -0.4 844,670 + RR42_RS24565 0.58 -1.4 844,670 + RR42_RS24565 0.58 +0.9 844,671 - RR42_RS24565 0.58 +0.0 844,710 - RR42_RS24565 0.61 -1.9 844,710 - RR42_RS24565 0.61 -0.9 844,925 + RR42_RS24565 0.76 +0.4 844,970 + RR42_RS24565 0.79 -1.0 844,970 + RR42_RS24565 0.79 +0.1 844,971 - RR42_RS24565 0.79 -0.6 845,033 + RR42_RS24565 0.83 -0.6 845,034 - RR42_RS24565 0.83 +0.6 845,034 - RR42_RS24565 0.83 +1.0 845,034 - RR42_RS24565 0.83 +1.3 845,111 + RR42_RS24565 0.89 -1.4 845,111 + RR42_RS24565 0.89 +0.4 845,112 - RR42_RS24565 0.89 -0.8 845,112 - RR42_RS24565 0.89 +0.3 845,112 - RR42_RS24565 0.89 -0.8 845,112 - RR42_RS24565 0.89 -0.5 845,174 + -0.1 845,174 + -1.3 845,175 - -0.5 845,318 - -0.4 845,382 + +0.1 845,674 - RR42_RS24570 0.39 -1.5 845,744 - RR42_RS24570 0.47 +1.7 845,813 + RR42_RS24570 0.54 +0.1 845,853 + RR42_RS24570 0.58 -0.2 845,854 - RR42_RS24570 0.58 +1.3 845,864 - RR42_RS24570 0.59 -0.1 845,864 - RR42_RS24570 0.59 -0.8 845,864 - RR42_RS24570 0.59 +0.3 845,898 - RR42_RS24570 0.62 -0.1 845,898 - RR42_RS24570 0.62 -1.7 845,980 - RR42_RS24570 0.71 -0.0 846,028 + RR42_RS24570 0.76 -1.4 846,062 + RR42_RS24570 0.79 -0.6 846,108 + RR42_RS24570 0.84 -0.1 846,220 + +0.6 846,235 + +0.2 846,236 - +2.0
Or see this region's nucleotide sequence